Time evolution of deformation in a human cartilage under cyclic loading

Recent imaging has revealed that in vivo contact deformations of human knee cartilage under physiological loadings are surprisingly large—typically on the order of 10%, but up to 20 or 30% of tibiofemora cartilage thickness depending on loading conditions. In this paper we develop a biphasic, large deformation, non-linear poroelastic model of cartilage that can accurately represent the time dependence and magnitude of cyclic cartilage deformations in vivo. The model takes into account cartilage tension–compression nonlinearity and a new constitutive relation in which the compressive stiffness and hydraulic permeability of the cartilage adjusts in response to the strain-dependent aggrecan concentration. The model predictions are validated using experimental test results on osteochondral plugs obtained from human cadavers. We find that model parameters can be optimised to give an excellent fit to the experimental data. Using typical hydraulic conductivity and stiffness parameters for healthy cartilage, we find that the experimentally observed transient and steady state tissue deformations under cyclic loading and unloading can be reproduced by the model. Steady state tissue deformations are shown to cycle between 10% (exudation strain) and 20% (total strain) in response to the cyclic test loads. At steady-state cyclic loading, the pore fluid exuded from the tissue is exactly equal to the pore fluid imbibed by the tissue during each load cycle

Osteoporotic bone fracture healing under the locking compression plate system

Abstract Osteoporosis is highly prevalent and a costly disease predicted to affect 1,555 million worldwide by 2050, and the total cost of osteoporotic fractures worldwide could reach US$131 billion by 2025. These statistics clearly affirm the significant economic burden of osteoporotic fractures to the community, and the need for the development of improved fracture treatments. Studies over the last decade reveal that, even though osteoporosis may not necessarily lead to non-union, it is associated with delayed fracture healing due to impaired mecho-regulation and angiogenesis in osteoporotic condition. Despite the advances in locking compression plate (LCP) technology, the operative treatment in osteoporotic fractures remains a challenge for an orthopaedic surgeon, often with unpredictable outcomes. Therefore, it becomes necessary to bridge the 'information gap' between osteoporosis and its effect on fracture healing, and so enables healing progression prediction under different fracture geometries and fixation configurations. By using a computational model of fracture healing, this paper demonstrates that fracture healing can be significantly delayed due to impaired mechano-regulation as a result of osteoporosis, and the impact of osteoporosis on fracture healing can be mitigated by adjusting the configuration of the LCP system to allow a certain degree of interfragmentary movement (IFM) without compromising overall fixation stability

Computational modelling of the mechanical environment of the early stage of fracture healing using structural engineering techniques

Bone healing is a complex biological process which is regulated by mechanical micro-environment caused by inter-fragmentary movement (IFM). IFM generated interstitial fluid flow within the fracture callus could potentially not only affect the mesenchymal stem cells migration and differentiation during the healing, but also enhance nutrient transport within the callus tissue. In this study, a three dimensional poroelastic finite element model of a human tibia was developed to study the mechanical behaviour of the fracture callus due to IFM at the early stage of fracture. The biophysical stimuli were characterised with three main parameters involved in the healing process: octahedral shear strain, interstitial fluid velocity and pressure. The proposed algorithm represents a first step towards to the development of a powerful simulation tool for fracture healing

The spatio-temporal mechanical environment of healthy and injured human cartilage during sustained activity and its role in cartilage damage

Recently we presented a computational model of articular cartilage calibrated for normal human tissue explants. This model was able to capture the transient deformation of cartilage experiencing a cyclic load. The model takes into account the tension-compression nonlinearity of the cartilage and incorporates the dependency of the compressive stiffness and fluid permeability of cartilage on the deformation-dependent aggrecan concentration in cartilage tissue. As such it represents a leading constitutive model of articular cartilage. Here we build on the previous study to develop an experimentally validated computational model to simulate mechanical consolidation response of intact and previously injured cartilage under sustained static loading, to develop our understanding of the implications for rates of tissue damage. We see that the type of prior injuries compromise the cartilage function in different ways. Relatively rapid consolidation is predicted for cartilage with a complete meniscectomy and that with a full thickness defect, indicating the inability of cartilage with such injuries to sustain interstitial fluid pressurisation for long periods of time, as does uninjured cartilage. By comparing the consolidation response of articular cartilage predicted by computational model against experimental measurements of the apparent friction coefficient following static loading, we find a strong linear positive correlation exists between cartilage degree of consolidation (DoC) and friction coefficient at the joint. As the DoC of articular cartilages can be estimated in vivo via medical imaging, the DoC can be used as an index to non-invasively evaluate the apparent friction coefficient between opposing cartilage surfaces, and so estimate the likelihood of frictional surface wear and/or cartilage damag

Investigation of role of cartilage surface polymer brush border in lubrication of biological joints

Although experimental evidence has suggested that the polymer brush border (PBB) on the cartilage surface is important in regulating fluid permeability in the contact gap, the current theoretical understanding of joint lubrication is still limited. To address this research gap, a multiscale cartilage contact model that includes PBB, in particular its effect on the fluid permeability of the contact gap, is developed in this study. Microscale modeling is employed to estimate the permeability of the contact gap. This permeability is classified into two categories: For a gap size > 1 µm, the flow resistance is assumed to be dominated by the cartilage roughness; for gap size < 1 µm, flow resistance is assumed to be dominated by the surface polymers extending beyond the collagen network of the articular cartilage. For gap sizes of less than 1 µm, the gap permeability decreases exponentially with increasing aggrecan concentration, whereas the aggrecan concentration varies inversely with the gap size. Subsequently, the gap permeability is employed in a macroscale cartilage contact model, in which both the contact gap space and articular cartilage are modeled as two interacting poroelastic systems. The fluid exchange between these two media is achieved by imposing pressure and normal flux continuity boundary conditions. The model results suggest that PBB can substantially enhance cartilage lubrication by increasing the gap fluid load support (e.g., by 26 times after a 20-min indentation compared with the test model without a PBB). Additionally, the fluid flow resistance of PBB sustains the cartilage interstitial fluid pressure for a relatively long period, and hence reduces the vertical deformation of the tissue. Furthermore, it can be inferred that a reduction in the PBB thickness impairs cartilage lubrication ability

Experimental investigation of the mechanical performance of novel rigid connections in prestressed circular composite precast concrete columns under cyclic lateral loading

The present study introduces an advanced design of Prestressed Circular Composite Precast Concrete Columns (PCCPCCs), which have been applied in real-world structures, equipped with two novel rigid connection types (i.e., the reinforcing-cage connection and the welded-plate connection) to address existing challenges in the design, construction, and quality control of traditional precast concrete (PC) columns. Five full-scale specimens were experimented individually to assess the mechanical performance of PCCPCCs subjected to cyclic lateral loading and constant axial loading. The results indicate that the specimens exhibited no signs of collapse or loss in axial load capacity during the tests. Furthermore, all specimens met or exceeded the performance criteria set by relevant standards, such as GB50011-2010 and ACI 374.1-05, regarding drift ratio, moment capacity, energy dissipation, and ductility. It demonstrates that the novel design of PCCPCCs proposed in this study can be integrated into practical construction projects designed as lateral-force-resisting systems, such as moment-resisting frames and bridges, to accommodate diverse construction project requirements

Computational Modelling for Managing Pathways to Cartilage Failure.

Over several decades the perception and therefore description of articular cartilage changed substantially. It has transitioned from being described as a relatively inert tissue with limited repair capacity, to a tissue undergoing continuous maintenance and even adaption, through a range of complex regulatory processes. Even from the narrower lens of biomechanics, the engagement with articular cartilage has changed from it being an interesting, slippery material found in the hostile mechanical environment between opposing long bones, to an intriguing example of mechanobiology in action. The progress revealing this complexity, where physics, chemistry, material science and biology are merging, has been described with increasingly sophisticated computational models. Here we describe how these computational models of cartilage as an integrated system can be combined with the approach of structural reliability analysis. That is, causal, deterministic models placed in the framework of the probabilistic approach of structural reliability analysis could be used to understand, predict, and mitigate the risk of cartilage failure or pathology. At the heart of this approach is seeing cartilage overuse and disease processes as a 'material failure', resulting in failure to perform its function, which is largely mechanical. One can then describe pathways to failure, for example, how homeostatic repair processes can be overwhelmed leading to a compromised tissue. To illustrate this 'pathways to failure' approach, we use the interplay between cartilage consolidation and lubrication to analyse the increase in expected wear rates associated with cartilage defects or meniscectomy

Computational study on synovial fluid flow behaviour in cartilage contact gap under osteoarthritic condition

This study numerically investigates the pathological changes of fluid flow in cartilage contact gap due to the changes in cartilage surface roughness and synovial fluid characteristics in osteoarthritic (OA) condition. First, cartilage surface topographies in both healthy and OA conditions are constructed using a numerical approach with consideration of both vertical and horizontal roughness. Then, constitutive equations for synovial fluid viscosity are obtained through calibration against previous experimental data. Finally, the roughness and synovial fluid information are input into the gap flow model to predict the gap permeability. The results show that the rougher surface of OA cartilage tends to decrease gap permeability by around 30%–60%. More importantly, with the reduction in gap size, the decrease in gap permeability becomes more significant, which could result in an early fluid ultrafiltration into the tissue. Moreover, it is demonstrated that the pathological synovial fluid has more deleterious effects on the gap permeability than the OA cartilage surface, as it could potentially increase the gap permeability by a few hundred times for pressure gradients less than 106 Pa/m, which could inhibit the fluid ultrafiltration into the tissue. The outcomes from this research indicate that the change in fluid flow behaviour in contact gap in OA condition could significantly affect the function of articular joints

Role of Dynamic Loading on Early Stage of Bone Fracture Healing

After fracture, mesenchymal stem cells (MSCs) and growth factors migrate into the fracture callus to exert their biological actions. Previous studies have indicated that dynamic loading induced tissue deformation and interstitial fluid flow could produce a biomechanical environment which significantly affects the healing outcomes. However, the fundamental relationship between the various loading regimes and different healing outcomes has not still been fully understood. In this study, we present an integrated computational model to investigate the effect of dynamic loading on early stage of bone fracture healing. The model takes into account cell and growth factor transport under dynamic loading, and mechanical stimuli mediated MSC differentiation and tissue production. The developed model was firstly validated by the available experimental data, and then implemented to identify the loading regimes that produce the optimal healing outcomes. Our results demonstrated that dynamic loading enhances MSC and growth factor transport in a spatially dependent manner. For example, compared to free diffusion, dynamic loading could significantly increase MSCs concentration in endosteal zone; and chondrogenic growth factors in both cortical and periosteal zones in callus. Furthermore, there could be an optimal dynamic loading regime (e.g. 10% strain at 1 Hz) which could potentially significant enhance endochondral ossification

A coupled contact model of cartilage lubrication in the mixed-mode regime under static compression

This study presents a coupled cartilage contact model, in which the contact gap and cartilage tissue are modelled as two poroelastic systems, linked by pressure and normal flux continuity boundary conditions. Using a tibial plug under indentation as a proof-of-concept model, the predictions support the weeping lubrication theory under static compression. Specifically, the interstitial fluid would exude from the underlying cartilage into the contact gap to extend the mixed-mode duration by > 20-fold compared to a no fluid exudation counterpart. Moreover, the traditional contact model, that does not consider the contact gap and cartilage fluid exchange, potentially overestimates the interstitial fluid pressure compared to the proposed coupled model. Parametric studies suggest that the increasing viscosity of synovial fluid prolongs the gap fluid pressurisation, while increasing the asperity stiffness reduces the gap fluid pressure but increases contact gap height