32 research outputs found

    Changes in Metabolic Stability of Structural and Enzyme Proteins in Experimental Hepatomegaly

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    The effect of mercury chloride and methyl mercury on brain microsomal Na<sup>+</sup>-K<sup>+</sup>-ATPase after partial delipidisation with Lubrol<sup>&reg;</sup>.

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    The microsomal Na+-K+-ATPase of rat brain was inhibited by mercury chloride and methyl mercury. The IC50 was 6.5 &times; 10-7 M for mercury chloride and 3.5 &times; 10-6 M for methyl mercury. The inhibition was of a non-competetive type with respect to ATP. The non-ionic detergent LubrolrGpotentiatedtheinhibitoryeffectofbothmercurials.ItisconcludedthatLubrolrG potentiated the inhibitory effect of both mercurials. It is concluded that LubrolrG removes the bulk lipids present outside the catalytic center of the enzyme. Consequently, the enzyme will become more sensitive to the inhibition by both mercurials
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