358 research outputs found
Occurrence of Salmonella spp. in flies and foodstuff from pork butcheries in Kampala, Uganda
Food-borne diseases such as salmonellosis are a major cause of human gastroenteritis worldwide, especially in the developing world due to poor sanitary conditions. Flies feed on food and breed in feces and other organic material. As such they are known vectors of Salmonella spp. Given that pork consumption in Uganda is rapidly increasing while good food safety practices remain absent, this study aims to assess the occurrence of Salmonella spp. in pork butcheries as a contribution to improve hygiene.
Seventy-seven pork butcheries out of 179 mapped in a previous survey in Kampala were randomly selected. From June–October 2014, samples of house flies, foodstuff and equipment were collected from all butcheries. Cultural isolation of Salmonella spp. was performed according to ISO 6579:2002.
Among 693 samples, 64 (9%) tested positive for Salmonella enteritidis. Among the positives, 32% were samples of raw pork (25), 25% flies’ midguts (19), less than 9% water (7), tomatoes (6), cabbage (4), onions (2) and one case on roasted pork1, respectively. Positive flies coincided with contaminated foodstuff in 29% of the butcheries. All 154 samples from either butchers’ hands or their equipment were negative for Salmonella spp.
The prevalence of S. enteritidis, especially on raw pork and in flies, illustrates the need for improving food safety in pork butcheries. Further research is required clarifying the gaps; especially the role of flies as microbiological carriers. In this context investigations are ongoing to identify Salmonella serotypes and their antimicrobial drug-resistance situation. However, these findings merit increased attention and can be used to improve knowledge, attitudes and practices amongst butchers.
The research was carried out with the financial support of the Federal Ministry for Economic Cooperation and Development (BMZ), Germany, and the CGIAR Research Program on Agriculture for Nutrition and Health, led by the International Food Policy Research Institute, through the Safe Food, Fair Food project at ILRI. Martin Heilmann got a scholarship from the German Academic Exchange Service (DAAD)
Antimicrobial resistance of Salmonella enterica in pork and vegetable servings at pork joints in Kampala, Uganda
The aim of this study was to investigate the prevalence and antimicrobial resistance
of Salmonella enterica subsp. enterica serovars including the presence of shared
plasmids in pork and related fresh vegetables served in pork joints in Kampala,
Uganda. Pork butcheries in three of the five administrative divisions of Kampala were
included for the survey. Samples included raw pork, roasted pork, water, onions,
tomatoes, cabbage, butcher’s hands (swabs), utensils (swabs) and fly midgut
extracts. A total of 693 samples were collected from 77 pork butcheries from June-
October 2014.
Overall 53.2% pork joints had samples positive for Salmonella enterica subsp.
enterica serovars. Isolation rates ranged from 31.2% (24/77) for raw pork, 1.3%
(1/77) for roasted pork, 7.8% (6/77) for tomatoes, 2.6% (2/77) for onions, 5.2% (4/77)
for cabbage, 9.1% (7/77) in water, and 22.1% (17/77) from fly midguts. Swab
samples taken from utensil and butcher’s hands swabs were found negative
(Heilmann et al., 2015).
In the isolates obtained, resistance towards 22 antibiotics was tested. Resistances
were found towards 11 out of the 22 antibiotics tested. High resistances were found
to Cephazolin (97%), Cefotixime (93%), Gentamicin (88%), and Ceftazidime (86%).
Intermediate resistance was found to Ciprofloxacin (59%) and Amoxicillin-Clavulanic
acid (57.6%). Most isolates (85%) were susceptible to Levofloxacin, Ofloxacin,
Sulfamethoxazole and Trimethoprim. Identification of plasmids by PCR-based
replicon typing was performed recognizing FIA,FIB,FIC,HI1,HI2,I1-
1ᵞ,L/M,N,P,W,T,A/C,K,B/O,X,Y,F and FIIA. Six incompatibility groups were identified:
FIA, W, FIC, FIB, P, Y with more than one incompatibility group existing among
different isolates.
A high resistance rate among Salmonella strains was found while the total number of
incompatibility groups detected was with approximation 2.4. Thus, even though the
total number of plasmids per strain is low, resistance rates detected remain high. The
high resistance rates are probably resulting from intensified food animal production
driving a greater use of antibiotics, which is a crucial aspect of public health concern
Direct Measurement of the Top Quark Mass at D0
We determine the top quark mass m_t using t-tbar pairs produced in the D0
detector by \sqrt{s} = 1.8 TeV p-pbar collisions in a 125 pb^-1 exposure at the
Fermilab Tevatron. We make a two constraint fit to m_t in t-tbar -> b W^+bbar
W^- final states with one W boson decaying to q-qbar and the other to e-nu or
mu-nu. Likelihood fits to the data yield m_t(l+jets) = 173.3 +- 5.6 (stat) +-
5.5 (syst) GeV/c^2. When this result is combined with an analysis of events in
which both W bosons decay into leptons, we obtain m_t = 172.1 +- 5.2 (stat) +-
4.9 (syst) GeV/c^2. An alternate analysis, using three constraint fits to fixed
top quark masses, gives m_t(l+jets) = 176.0 +- 7.9 (stat) +- 4.8 (syst)
GeV/C^2, consistent with the above result. Studies of kinematic distributions
of the top quark candidates are also presented.Comment: 43 pages, 53 figures, 33 tables. RevTeX. Submitted to Phys. Rev.
Measurement of the Boson Mass
A measurement of the mass of the boson is presented based on a sample of
5982 decays observed in collisions at
= 1.8~TeV with the D\O\ detector during the 1992--1993 run. From a
fit to the transverse mass spectrum, combined with measurements of the
boson mass, the boson mass is measured to be .Comment: 12 pages, LaTex, style Revtex, including 3 postscript figures
(submitted to PRL
Search for a Fourth Generation Charge -1/3 Quark via Flavor Changing Neutral Current Decay
We report on a search for pair production of a fourth generation charge -1/3
quark (b') in pbar p collisions at sqrt(s) = 1.8 TeV at the Fermilab Tevatron
using an integrated luminosity of 93 pb^-1. Both quarks are assumed to decay
via flavor changing neutral currents (FCNC). The search uses the signatures
gamma + 3 jets + mu-tag and 2 gamma + 2 jets. We see no significant excess of
events over the expected background. We place an upper limit on the production
cross section times branching fraction that is well below theoretical
expectations for a b' quark decaying exclusively via FCNC for b' quark masses
up to m(Z) + m(b).Comment: Eleven pages, two postscript figures, submitted to Physical Review
Letter
Search for First Generation Scalar Leptoquark Pairs in ppbar Collisions at sqrt(s)=1.8 TeV
We have searched for first generation scalar leptoquark (LQ) pairs in the
enu+jets channel using ppbar collider data (integrated luminosity= 115 pb^-1)
collected by the DZero experiment at the Fermilab Tevatron during 1992-96. The
analysis yields no candidate events. We combine the results with those from the
ee+jets and nunu+jets channels to obtain 95% confidence level (CL) upper limits
on the LQ pair production cross section as a function of mass and of beta, the
branching fraction to a charged lepton. Comparing with the next-to-leading
order theory, we set 95% CL lower limits on the LQ mass of 225, 204, and 79
GeV/c^2 for beta=1, 1/2, and 0, respectively.Comment: 14 pages, 4 figures, submitted to Physical Review Letters Replaced to
correct visitor addresse
Search for Top Squark Pair Production in the Dielectron Channel
This report describes the first search for top squark pair production in the
channel stop_1 stopbar_1 -> b bbar chargino_1 chargino_1 -> ee+jets+MEt using
74.9 +- 8.9 pb^-1 of data collected using the D0 detector. A 95% confidence
level upper limit on sigma*B is presented. The limit is above the theoretical
expectation for sigma*B for this process, but does show the sensitivity of the
current D0 data set to a particular topology for new physics.Comment: Five pages, including three figures, submitted to PRD Brief Report
Limits on Anomalous WWgamma and WWZ Couplings
Limits on the anomalous WWgamma and WWZ couplings are presented from a
simultaneous fit to the data samples of three gauge boson pair final states in
pbar-p collisions at sqrt(s)=1.8 TeV: Wgamma production with the W boson
decaying to enu or munu, W boson pair production with both of the W bosons
decaying to enu or munu, and WW or WZ production with one W boson decaying to
enu and the other W boson or the Z boson decaying to two jets. Assuming
identical WWgamma and WWZ couplings, 95 % C.L. limits on the anomalous
couplings of -0.30<Delta kappa<0.43 (lambda = 0) and -0.20<lambda<0.20 (Delta
kappa = 0) are obtained using a form factor scale Lambda = 2.0 TeV. Limits
found under other assumptions on the relationship between the WWgamma and WWZ
couplings are also presented.Comment: 13 pages, 3 figures, submitted to Physical Review
Second Generation Leptoquark Search in p\bar{p} Collisions at = 1.8 TeV
We report on a search for second generation leptoquarks with the D\O\
detector at the Fermilab Tevatron collider at = 1.8 TeV.
This search is based on 12.7 pb of data. Second generation leptoquarks
are assumed to be produced in pairs and to decay into a muon and quark with
branching ratio or to neutrino and quark with branching ratio
. We obtain cross section times branching ratio limits as a function
of leptoquark mass and set a lower limit on the leptoquark mass of 111
GeV/c for and 89 GeV/c for at the 95%\
confidence level.Comment: 18 pages, FERMILAB-PUB-95/185-
Search for Production via Trilepton Final States in collisions at TeV
We have searched for associated production of the lightest chargino,
, and next-to-lightest neutralino, , of the
Minimal Supersymmetric Standard Model in collisions at
\mbox{ = 1.8 TeV} using the \D0 detector at the Fermilab Tevatron
collider. Data corresponding to an integrated luminosity of 12.5 \ipb
were examined for events containing three isolated leptons. No evidence for
pair production was found. Limits on
BrBr are
presented.Comment: 17 pages (13 + 1 page table + 3 pages figures). 3 PostScript figures
will follow in a UUEncoded, gzip'd, tar file. Text in LaTex format. Submitted
to Physical Review Letters. Replace comments - Had to resumbmit version with
EPSF directive
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