82 research outputs found
Fatigue Index is Affected by Repetitive Transcranial Magnetic Stimulation Target but not Protocol
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Identification of a Novel Class of Farnesylation Targets by Structure-Based Modeling of Binding Specificity
Farnesylation is an important post-translational modification catalyzed by farnesyltransferase (FTase). Until recently it was believed that a C-terminal CaaX motif is required for farnesylation, but recent experiments have revealed larger substrate diversity. In this study, we propose a general structural modeling scheme to account for peptide binding specificity and recapitulate the experimentally derived selectivity profile of FTase in vitro. In addition to highly accurate recovery of known FTase targets, we also identify a range of novel potential targets in the human genome, including a new substrate class with an acidic C-terminal residue (CxxD/E). In vitro experiments verified farnesylation of 26/29 tested peptides, including both novel human targets, as well as peptides predicted to tightly bind FTase. This study extends the putative range of biological farnesylation substrates. Moreover, it suggests that the ability of a peptide to bind FTase is a main determinant for the farnesylation reaction. Finally, simple adaptation of our approach can contribute to more accurate and complete elucidation of peptide-mediated interactions and modifications in the cell
Do Less-Violent Technologies Result in Less Violence? A Theoretical Investigation Applied to the Use of Tasers by Law Enforcement
International prevalence of adverse drug events in hospitals: an analysis of routine data from England, Germany, and the USA
Maternal smoking during pregnancy and birth defects in children: a systematic review with meta-analysis
Inter- and Intraspecific Variations in Wild Rice Species Detected Through Isozyme Markers
Effects of Repetitive Transcranial Magnetic Stimulation on Mood When Targeting Motor-Related Brain Regions
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Corticospinal Excitability and the Size of Motor Cortex Representation Reflect Distinct Aspects of Corticomotor Function
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Little Variability in Active Motor Thresholds of Trunk and Leg Muscles Across Days
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