Ml proteins from Mesorhizobium loti and MucR from Brucella abortus: an AT-rich core DNA-target site and oligomerization ability

Mesorhizobium loti contains ten genes coding for proteins sharing high amino acid sequence identity with members of the Ros/MucR transcription factor family. Five of these Ros/MucR family members from Mesorhizobium loti (Ml proteins) have been recently structurally and functionally characterized demonstrating that Ml proteins are DNA-binding proteins. However, the DNA-binding studies were performed using the Ros DNA-binding site with the Ml proteins. Currently, there is no evidence as to when the Ml proteins are expressed during the Mesorhizobium loti life cycle as well as no information concerning their natural DNA-binding site. In this study, we examine the ml genes expression profile in Mesorhizobium loti and show that ml1, ml2, ml3 and ml5 are expressed during planktonic growth and in biofilms. DNA-binding experiments show that the Ml proteins studied bind a conserved AT-rich site in the promoter region of the exoY gene from Mesorhizobium loti and that the proteins make important contacts with the minor groove of DNA. Moreover, we demonstrate that the Ml proteins studied form higher-order oligomers through their N-terminal region and that the same AT-rich site is recognized by MucR from Brucella abortus using a similar mechanism involving contacts with the minor groove of DNA and oligomerization

Professional Learning Through Everyday Work: How Finance Professionals Self-Regulate Their Learning

Professional learning is a critical component of ongoing improvement and innovation and the adoption of new practices in the workplace. Professional learning is often achieved through learning embedded in everyday work tasks. However, little is known about how professionals self-regulate their learning through regular work activities. This paper explores how professionals in the finance sector (n-30) self-regulate their learning through day-to-day work. Analysis focuses on three sub-processes of self-regulated learning that have been identified as significant predictors of good self-regulated learning at work. A key characteristic of good self-regulation is viewing learning as a form of long-term, personalised self-improvement. This study provides a foundation for future policy and planning in organisations aiming to encourage self-regulated learning

Tracing digital transformation in educational organizations

This chapter describes the quantitative approaches for assessing various aspects of digital transformation from the joint research and development project #ko.vernetzt. In an application-oriented case study approach, the operationalization of a maturity model of digital transformation for educational organizations (MMEO) and the design and implementation of a multi-perspective evaluation concept are outlined. While the MMEO provides a state perspective on the digital transformation of an educational organization and its employees, the evaluation concept aims at tracing developments of media-related professional competencies. MMEO has been implemented in a study with N = 222 participants, while N = 59 learners were subject to the evaluation polls. The results provide the necessary evidence for implementing and continuously improving a qualification program in the regarded research context. However, the methodology can also be transferred to other organizations in the education sector and beyond

Compendium of Structural Testing Data for 20-Psi Coal Mine Seals

This report presents nearly all structural data available from explosion tests of 20-psi mine ventilation seals and concrete-block ventilation stoppings that were conducted by the National Institute for Occupational Safety and Health during 1997-2008. Although the seals tested were designed to meet the former federal 20-psi pressure design standard, the structural information contained herein on these seal tests will facilitate the analysis and design of coal mine seals that meet the new explosion pressure design criteria of 50 and 120 psi as set forth in the Mine Safety and Health Administration (MSHA)\u27s final rule on Sealing of Abandoned Areas. The seal testing data are organized into six broad categories of seal structures based on the materials used and the construction method for those 20-psi seals: 1. Concretelike materials with steel reinforcement and reinforcement bar anchorage to rock; 2. Pumpable cementitious materials of varying compressive strengths with no steel reinforcement and no hitching; 3. Articulated structures such as solid-concrete-block seals and ventilation stoppings made of solid and hollow-core concrete blocks; 4. Polymer and aggregate materials without hitching; 5. Wood-crib-block seals with or without hitching; 6. Articulated structures such as lightweight blocks with or without hitching. This summary contains data on 52 different structures in the above categories - 44 seals and 8 ventilation stoppings. The structural data sets include the applied loading on the tested seal represented by a pressure-time curve and, when available, the measured seal response represented by a displacement-time curve. The structural data sets enable the calibration and verification of numerical models of seal behavior at the 20-psi level, which may then facilitate future structural analyses of seal designs for the new 50- and 120-psi explosion pressure design criteria

The role of human resource development in organizational change: Professional development strategies of employees, managers and HRD practitioners

The aim of this chapter is to present, and provide empirical evidence for, a theory that gives central stage to actors operating strategically in the context of professional development. The learning-network theory (based on the seminal work of Ferd van der Krogt) deals with the organization of HRD taking into account the various ways in which different actors employ their own professional development strategies. It also assumes that neither managers nor employees will have very explicit ideas about the organization of HRD (which HRD practitioners tend to forget about in this connection). The chapter first presents the main tenets of the learning-network theory. Its proceeds by describing a case study conducted in a healthcare setting, where managers and HRD practitioners attempted to introduce a new working method through a learning program. The case study also shows how employees (healthcare officers) worked on their own professional development. The chapter ends in a discussion of the different strategies employed by employees, managers, and HRD practitioners in organizing HRD

Passive Mine Blast Attenuators Constructed of Rock Rubble for Protecting Ventilation Seals

The design requirements for mine ventilation seals have undergone a radical transformation in recent years, and these revisions have greatly increased the cost of the seal designs and their construction. For example, in the past two years, new federal regulations have increased the minimum design requirement to withstand explosion pressures from 138 kPa (20 psig) to 345 kPa (50 psig) or 827 kPa (120 psig), depending on whether the sealed mine volume is monitored continuously or not. Moreover, there is still a possibility that under certain conditions (such as detonations) even higher pressure requirements may be necessary. The ability of a monolithic, stand-alone mine seal to reliably withstand the full range of current and future pressures is becoming increasingly difficult and expensive. In an effort to develop a practical alternative, the West Virginia Office of Miners\u27 Health Safety and Training (WVOMHST) and the National Institute for Occupational Safety and Health (NIOSH) have collaborated in a research effort to develop a practical, economic, and safe mine sealing technique that can enable mines seals to meet the full range of new explosion pressure design requirements. The basic idea is to use a barrier of common mine gob and rubble in combination with a conventional mine seal so that the pressure resulting from a gas explosion is reflected, absorbed, and attenuated, so the pressure on the ventilation seal is reduced. This paper discusses some concepts and preliminary test results for a Passive Mine Blast Attenuator (PMBA) that can provide a useful alternative to increasingly larger and stronger stand-alone mine seals. Numerical models and full-scale experiments conducted at the NIOSH Lake Lynn Laboratory Experimental Mine (LLEM) show that the use of a PMBA can signficantly reduce the blast pressure and impulse on conventional ventilation seals. DISCLAIMER: This information is distributed solely for the purpose of pre-dissemination peer review under applicable information quality guidelines. It has not been formally disseminated by the National Institute for Occupational Safety and Health (NIOSH). It does not represent and should not be construed to represent any agency determination or policy