Low scale Seesaw model and Lepton Flavor Violating Rare B Decays

We study lepton flavor number violating rare B decays, $b \to s l_h^{\pm} l_l^{\mp}$, in a seesaw model with low scale singlet Majorana neutrinos motivated by the resonant leptogenesis scenario. The branching ratios of inclusive decays $b \to s l_h^{\pm} \bar{l_l}^{\mp}$ with two almost degenerate singlet neutrinos at TeV scale are investigated in detail. We find that there exists a class of seesaw model in which the branching fractions of $b \to s \tau \mu$ and $\tau \to \mu \gamma$ can be as large as $10^{-10}$ and $10^{-9}$ within the reach of Super B factories, respectively, without being in conflict with neutrino mixings and mass squared difference of neutrinos from neutrino data, invisible decay width of $Z$ and the present limit of $Br(\mu \to e \gamma)$.Comment: 19 pages, 6 figure

Cosmological Family Asymmetry and CP violation

We discuss how the cosmological baryon asymmetry can be achieved by the lepton family asymmetries of heavy Majorana neutrino decays and they are related to CP violation in neutrino oscillation, in the minimal seesaw model with two heavy Majorana neutrinos. We derive the most general formula for CP violation in neutrino oscillation in terms of the heavy Majorana masses and Yukawa mass term. It is shown that the formula is very useful to classify several models in which $e-$, $\mu-$ and $\tau-$leptogenesis can be separately realized and to see how they are connected with low energy CP violaton. To make the models predictive, we take texture with two zeros in the Dirac neutrino Yukawa matrix. In particular, we find some interesting cases in which CP violation in neutrino oscillation can happen while lepton family asymmetries do not exist at all. On the contrary, we can find $e-$, $\mu-$ and $\tau-$leptogenesis scenarios in which the cosmological CP violation and low energy CP violation measurable via neutrino oscillations are very closely related to each other. By determining the allowed ranges of the parameters in the models, we predict the sizes of CP violation in neutrino oscillation and $|V_{e3}^{MNS}|$. Finally, the leptonic unitarity triangles are reconstructed.Comment: 22 pages, 9 figures A figure caption correcte

Production of manganese peroxidase by white rot fungi from potato-processing wastewater: Role of amino acids on biosynthesis

The production of manganese peroxidase (MnP) by white rot fungus strain L-25 was carried out using potato-processing wastewater and the effects of amino acids in the potato-processing wastewater was investigated. The MnP was efficiently produced from the wastewater by the addition of glucose and the maximum MnP activity linearly increased with an increase in the glucose concentration. The initial pH affected the cell growth and also the production rate of the MnP. The maximum activity and theproduction rate of the MnP using the potato-processing wastewater-based medium were higher (ca. 2.5- fold) than that of the basal medium. Moreover, amino acids in the wastewater had significant effects onthe MnP production. L-Glutamic acid, L-aspartic acid and L-serine induced the MnP secretion, on the other hand, L-phenylalanine, L-tyrosine, L-leucine and L-lysine inhibited it. The addition of L-leucine and L-lysine caused growth inhibition, while, L-phenylalanine and L-tyrosine blocked the MnP biosynthetic pathway. Ammonium ion released from the L-phenylalanine by the L-phenylalanine ammonia-lyase participated in the repression of the MnP biosynthetic pathway of the strain L-25

QRDR mutations, efflux system & antimicrobial resistance genes in enterotoxigenic Escherichia coli isolated from an outbreak of diarrhoea in Ahmedabad, India

Background & objectives: Diverse mechanisms have been identified in enteric bacteria for their adaptation and survival against multiple classes of antimicrobial agents. Resistance of bacteria to the most effective fluoroquinolones have increasingly been reported in many countries. We have identified that most of the enterotoxigenic Escherichia coli (ETEC) were resistant to several antimicrobials in a diarrhoea outbreak at Ahmedabad during 2000. The present study was done to identify several genes responsible for antimicrobial resistance and mobile genetic elements in the ETEC strains. Methods: Seventeen ETEC strains isolated from diarrhoeal patients were included in this study. The antimicrobial resistance was confirmed by conventional disc diffusion method. PCR and DNA sequencing were performed for the identification of mutation in the quinolone resistance-determining regions (QRDRs). Efflux pump was tested by inhibiting the proton-motive force. DNA hybridization assay was made for the detection of integrase genes and the resistance gene cassettes were identified by direct sequencing of the PCR amplicons. Results: Majority of the ETEC had GyrA mutations at codons 83 and 87 and in ParC at codon 80. Six strains had an additional mutation in ParC at codon 108 and two had at position 84. Plasmid-borne qnr gene alleles that encode quinolone resistance were not detected but the newly described aac(6')-Ib-cr gene encoding a fluoroquinolne-modifying enzyme was detected in 64.7 per cent of the ETEC. Class 1 (intI1) and class 2 (intI2) integrons were detected in six (35.3%) and three (17.6%) strains, respectively. Four strains (23.5%) had both the classes of integrons. Sequence analysis revealed presence of dfrA17, aadA1, aadA5 in class 1, and dfrA1, sat1, aadA1 in class 2 integrons. In addition, the other resistance genes such as tet gene alleles (94.1%), catAI (70.6%), strA (58.8%), bla TEM-1 (35.2%), and aphA1-Ia (29.4%) were detected in most of the strains. Interpretation & Conclusions: Innate gene mutations and acquisition of multidrug resistance genes through mobile genetic elements might have contributed to the emergence of multidrug resistance (MDR) in ETEC. This study reinforces the necessity of utilizing molecular techniques in the epidemiological studies to understand the nature of resistance responsible for antimicrobial resistance in different species of pathogenic bacteria

Functional analysis of recombinant B beta 15C and B beta 15A fibrinogens demonstrates that B beta 15G residue plays important roles in FPB release and in lateral aggregation of protofibrils

The definitive version is available at www.blackwell-synergy.com.ArticleJOURNAL OF THROMBOSIS AND HAEMOSTASIS. 3(5): 983-990 (2005)journal articl

In vitro transcription of compound heterozygous hypofibrinogenemia Matsumoto IX; first identification of FGB IVS6 deletion of 4 nucleotides and FGG IVS3-2A > G causing abnormal RNA splicing

ArticleClinica Chimica Acta. 411(17-18):1325-1329 (2010)journal articl

Impact of sitagliptin on endometrial mesenchymal stem-like progenitor cells : a randomised, double-blind placebo-controlled feasibility trial

Background: Recurrent pregnancy loss (RPL) is associated with the loss of endometrial mesenchymal stem-like progenitor cells (eMSC). DPP4 inhibitors may increase homing and engraftment of bone marrow-derived cells to sites of tissue injury. Here, we evaluated the effect of the DPP4 inhibitor sitagliptin on eMSC in women with RPL, determined the impact on endometrial decidualization, and assessed the feasibility of a full-scale clinical trial. Methods: A double-blind, randomised, placebo-controlled feasibility trial on women aged 18 to 42 years with a history of 3 or more miscarriages, regular menstrual cycles, and no contraindications to sitagliptin. Thirty-eight subjects were randomised to either 100 mg sitagliptin daily for 3 consecutive cycles or identical placebo capsules. Computer generated, permuted block randomisation was used to allocate treatment packs. Colony forming unit (CFU) assays were used to quantify eMSC in midluteal endometrial biopsies. The primary outcome measure was CFU counts. Secondary outcome measures were endometrial thickness, study acceptability, and first pregnancy outcome within 12 months following the study. Tissue samples were subjected to explorative investigations. Findings: CFU counts following sitagliptin were higher compared to placebo only when adjusted for baseline CFU counts and age (RR: 1.52, 95% CI: 1.32–1.75, P<0.01). The change in CFU count was 1.68 in the sitagliptin group and 1.08 in the placebo group. Trial recruitment, acceptability, and drug compliance were high. There were no serious adverse events. Explorative investigations showed that sitagliptin inhibits the expression of DIO2, a marker gene of senescent decidual cells. Interpretation: Sitagliptin increases eMSCs and decreases decidual senescence. A large-scale clinical trial evaluating the impact of preconception sitagliptin treatment on pregnancy outcome in RPL is feasible and warranted. Funding: Tommy's Baby Charity. Clinical trial registration: EU Clinical Trials Register no. 2016-001120-54

Development of Time- and Energy-Resolved Synchrotron-Radiation-Based Mössbauer Spectroscopy

14th International Conference on Synchrotron Radiation Instrumentation (SRI 2021) 28.03.2022 - 01.04.2022 OnlineSynchrotron-radiation based Mössbauer spectroscopy has become a useful technique capable for investigating various Mössbauer isotopes. For a typical experimental setup, the information associated with the pulse height (that is, energy) in an avalanche photodiode (APD) detector has not been used effectively. By using a system for simultaneous measurement system of time and energy associated with the APD signal, a system for the time- and energy-resolved Mössbauer spectroscopy has been developed. In this system, the pulse height information was converted to the time information through an amplitude-to-time converter applied to one of the divided signals from the APD. The corresponding time information was processed separately from another one of the divided signals. Both signals are recorded by a multi-channel scaler in an event-by-event data acquisition process. The velocity information from the Mössbauer transducer was also recorded as a tag for each signal event. Thus, the Mössbauer spectra with any time- and energy-window can be reconstructed after the data collection process. This system can be used for many purposes in time- and energy-resolved Mössbauer spectroscopy, and shows significant promise for use with other fast detectors and for various types of experiments

Do restoration techniques and types of weed control influence the composition of edaphic entomofauna?

Agroforestry systems (AFS) are generally referred to as systems where perennial plants are associated with annual or perennial crops, spanning from relatively simple agroforestry systems characterized by only two associated species to very complex ones, close to natural systems. However, there are few studies on edaphic entomofauna in newly implanted restoration systems. We investigated the composition of edaphic entomofauna in areas managed under different restoration techniques (agroforestry system or mixed-planting) and types of weed control (chemical or mowing). In each treatment, we performed collections with pitfall traps in July and December 2017. A total of 11,727 specimens distributed in 11 orders and 45 families were collected. Most of the individuals collected were Collembola (53.86%) and Hymenoptera: Formicidae (31.50%). No significant interaction was observed between restoration techniques and types of weed control combined. However, for abundance, both restoration techniques and types of weed control were individually significant, with chemical control showing a higher abundance in relation to mowing, and agroforestry concerning mixed-planting. There was no significant difference in richness in any of the variables studied. We conclude that the agroforestry system and the chemical control can be viable for edaphic entomofauna, although future research is necessary to evaluate the dynamic of edaphic entomofauna during the development of agroforestry systems

Flavoured soft leptogenesis and natural values of the B term

We revisit flavour effects in soft leptogenesis relaxing the assumption of universality for the soft supersymmetry breaking terms. We find that with respect to the case in which the heavy sneutrinos decay with equal rates and equal CP asymmetries for all lepton flavours, hierarchical flavour configurations can enhance the efficiency by more than two orders of magnitude. This translates in more than three order of magnitude with respect to the one-flavour approximation. We verify that lepton flavour equilibration effects related to off-diagonal soft slepton masses are ineffective for damping these large enhancements. We show that soft leptogenesis can be successful for unusual values of the relevant parameters, allowing for $B\sim {\cal O}({\rm TeV})$ and for values of the washout parameter up to $m_{\rm eff}/m_* \sim 5\times 10^{3}$.Comment: 23 pages, 5 figures postscript, Minor changes to match the published version in JHE