17,636 research outputs found
The Porphyromonas gingivalis hemagglutinins HagB and HagC are major mediators of adhesion and biofilm formation
Porphyromonas gingivalis is a bacterium associated with chronic periodontitis that possesses a family of genes encoding hemagglutinins required for heme acquisition. In this study we generated ΔhagB and ΔhagC mutants in strain W83 and demonstrate that both hagB and hagC are required for adherence to oral epithelial cells. Unexpectedly, a double ΔhagB/ΔhagC mutant had less severe adherence defects than either of the single mutants, but was found to exhibit increased expression of the gingipain-encoding genes rgpA and kgp, suggesting that a ΔhagB/ΔhagC mutant is only viable in populations of cells that exhibit increased expression of genes involved in heme acquisition. Disruption of hagB in the fimbriated strain ATCC33277 demonstrated that HagB is also required for stable attachment of fimbriated bacteria to oral epithelial cells. Mutants of hagC were also found to form defective single and multi-species biofilms that had reduced biomass relative to biofilms formed by the wild-type strain. This study highlights the hitherto unappreciated importance of these genes in oral colonization and biofilm formation
Jets or high velocity flows revealed in high-cadence spectrometer and imager co-observations?
We report on active region EUV dynamic events observed simultaneously at
high-cadence with SUMER/SoHO and TRACE. Although the features appear in the
TRACE Fe ix/x 171A images as jets seen in projection on the solar disk, the
SUMER spectral line profiles suggest that the plasma has been driven along a
curved large scale magnetic structure, a pre-existing loop. The SUMER
observations were carried out in spectral lines covering a large temperature
range from 10^4 K to 10^6 K. The spectral analysis revealed that a sudden
heating from an energy deposition is followed by a high velocity plasma flow.
The Doppler velocities were found to be in the range from 90 to 160 km/s. The
heating process has a duration which is below the SUMER exposure time of 25 s
while the lifetime of the events is from 5 to 15 min. The additional check on
soft X-ray Yohkoh images shows that the features most probably reach 3 MK
(X-ray) temperatures. The spectroscopic analysis showed no existence of cold
material during the events
Nucleation at the DNA supercoiling transition
Twisting DNA under a constant applied force reveals a thermally activated
transition into a state with a supercoiled structure known as a plectoneme.
Using transition state theory, we predict the rate of this plectoneme
nucleation to be of order 10^4 Hz. We reconcile this with experiments that have
measured hopping rates of order 10 Hz by noting that the viscosity of the bead
used to manipulate the DNA limits the measured rate. We find that the intrinsic
bending caused by disorder in the base-pair sequence is important for
understanding the free energy barrier that governs the transition. Both
analytic and numerical methods are used in the calculations. We provide
extensive details on the numerical methods for simulating the elastic rod model
with and without disorder.Comment: 18 pages, 15 figure
Vibrational Stability of NLC Linac accelerating structure
The vibration of components of the NLC linac, such as accelerating structures
and girders, is being studied both experimentally and analytically. Various
effects are being considered including structural resonances and vibration
caused by cooling water in the accelerating structure. This paper reports the
status of ongoing work.Comment: 3 pages 8 figures Presented at EPAC 2002 Paris Franc
L\'evy walks and scaling in quenched disordered media
We study L\'evy walks in quenched disordered one-dimensional media, with
scatterers spaced according to a long-tailed distribution. By analyzing the
scaling relations for the random-walk probability and for the resistivity in
the equivalent electric problem, we obtain the asymptotic behavior of the mean
square displacement as a function of the exponent characterizing the scatterers
distribution. We demonstrate that in quenched media different average
procedures can display different asymptotic behavior. In particular, we
estimate the moments of the displacement averaged over processes starting from
scattering sites, in analogy with recent experiments. Our results are compared
with numerical simulations, with excellent agreement.Comment: Phys. Rev. E 81, 060101(R) (2010
Realization of Coherent Optically Dense Media via Buffer-Gas Cooling
We demonstrate that buffer-gas cooling combined with laser ablation can be
used to create coherent optical media with high optical depth and low Doppler
broadening that offers metastable states with low collisional and motional
decoherence. Demonstration of this generic technique opens pathways to coherent
optics with a large variety of atoms and molecules. We use helium buffer gas to
cool 87Rb atoms to below 7 K and slow atom diffusion to the walls.
Electromagnetically induced transparency (EIT) in this medium allows for 50%
transmission in a medium with initial OD >70 and for slow pulse propagation
with large delay-bandwidth products. In the high-OD regime, we observe
high-contrast spectrum oscillations due to efficient four-wave mixing.Comment: 4 pages, 4 figures. V2: modified title, abstract, introduction,
conclusion; added references; improved theoretical fit in figure 3(b);
shortened slow light theory description; clarified simplicity of apparatus.
Final version as published in Phys. Rev.
Large spin relaxation rates in trapped submerged-shell atoms
Spin relaxation due to atom-atom collisions is measured for magnetically
trapped erbium and thulium atoms at a temperature near 500 mK. The rate
constants for Er-Er and Tm-Tm collisions are 3.0 times 10^-10 cm^3 s^-1 and 1.1
times 10^-10 cm^3 s^-1, respectively, 2-3 orders of magnitude larger than those
observed for highly magnetic S-state atoms. This is strong evidence for an
additional, dominant, spin relaxation mechanism, electrostatic anisotropy, in
collisions between these "submerged-shell" L > 0 atoms. These large spin
relaxation rates imply that evaporative cooling of these atoms in a magnetic
trap will be highly inefficient.Comment: 10 pages, 3 figure
Hydrogel microparticles from lithographic processes: Novel materials for fundamental and applied colloid science
In recent years, there has been a surge in methods to synthesize geometrically and chemically complex microparticles. Analogous to atoms, the concept of a “periodic table” of particles has emerged and continues to be expanded upon. Complementing the natural intellectual curiosity that drives the creation of increasingly intricate particles is the pull from applications that take advantage of such high-value materials. Complex particles are now being used in fields ranging from diagnostics and catalysis, to self-assembly and rheology, where material composition and microstructure are closely linked with particle function. This is especially true of polymer hydrogels, which offer an attractive and broad class of base materials for synthesis. Lithography affords the ability to engineer particle properties a priori and leads to the production of homogenous ensembles of particles. This review summarizes recent advances in synthesizing hydrogel microparticles using lithographic processes and highlights a number of emerging applications. We discuss advantages and limitations of current strategies, and conclude with an outlook on future trends in the field.National Science Foundation (U.S.) (Grant DMR-1006147)Novartis-MIT Center for Continuous ManufacturingNational Institute for Biomedical Imaging and Bioengineering (U.S.) (Grant R21EB008814
Macroinvertebrate community responses to a dewatering disturbance gradient in a restored stream
Dewatering disturbances are common in aquatic systems and represent a relatively untapped field of disturbance ecology, yet studying dewatering events along gradients in non-dichotomous (i.e. wet/dry) terms is often difficult. Because many stream restorations can essentially be perceived as planned hydrologic manipulations, such systems can make ideal test-cases for understanding processes of hydrological disturbance. In this study we used an experimental drawdown in a 440 ha stream/wetland restoration site to assess aquatic macroinvertebrate community responses to dewatering and subsequent rewetting. The geomorphic nature of the site and the design of the restoration allowed dewatering to occur predictably along a gradient and decoupled the hydrologic response from any geomorphic (i.e. habitat heterogeneity) effects. In the absence of such heterogeneous habitat refugia, reach-scale wetted perimeter and depth conditions exerted a strong control on community structure. The community exhibited an incremental response to dewatering severity over the course of this disturbance, which was made manifest not as a change in community means but as an increase in community variability, or dispersion, at each site. The dewatering also affected inter-species abundance and distributional patterns, as dewatering and rewetting promoted alternate species groups with divergent habitat tolerances. Finally, our results indicate that rapid rewetting – analogous to a hurricane breaking a summer drought – may represent a recovery process rather than an additional disturbance and that such processes, even in newly restored systems, may be rapid
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