Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique

Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. One interesting option may also be to use HILIC in orthogonal and/or two-dimensional separations. Bioapplications of HILIC systems are also presented

Real Time Blood Image Processing Application for Malaria Diagnosis Using Mobile Phones

This paper describes a fast and reliable mobile phone Android application platform for blood image analysis and malaria diagnosis from Giemsa stained thin blood film images. The application is based on novel Annular Ring Ratio Method which is already implemented, tested and validated in MATLAB. The method detects the blood components such as the Red Blood Cells (RBCs), White Blood Cells (WBCs), and identifies the parasites in the infected RBCs. The application also recognizes the different life stages of the parasites and calculates the parasitemia which is a measure of the extent of infection

Adaptive Rate Signal Acquisition and Denoising For Efficient Mobile Systems

International audienc

Exact non local expression for the wall heat transfer coefficient in tubular catalytic reactors

International audienceA new exact and non local expression for the wall Nusselt number is derived to have a deep insight into the physical mechanisms that govern the wall heat transfer. 3D high-fidelity numerical simulations (RANS) are then carried out in tubular packed beds and a new set of criteria is defined to extend the Representative Elementary Volume concept to packed bed configurations which enables to unequivocally up-scale 3D simulation data to the observation scale. The mean flow deviation and the mechanical dispersion are shown to play a key role in the wall heat transfer. Finally, the usual correlations of the form View the MathML source Nu=αRepβ found in the literature for the Nusselt number are validated through fine 3D simulations and on the basis of physical investigations for 4000 ⩽ Rep ⩽ 30,000

Neutrophil proteinase 3 and dipeptidyl peptidase I (cathepsin C) as pharmacological targets in granulomatosis with polyangiitis (Wegener granulomatosis).

Neutrophils are among the first cells implicated in acute inflammation. Leaving the blood circulation, they quickly migrate through the interstitial space of tissues and liberate oxidants and other antimicrobial proteins together with serine proteinases. Neutrophil elastase, cathepsin G, proteinase 3 (PR3), and neutrophil serine protease 4 are four hematopoietic serine proteases activated by dipeptidyl peptidase I during neutrophil maturation and are mainly stored in cytoplasmic azurophilic granules. They regulate inflammatory and immune responses after their release from activated neutrophils at inflammatory sites. Membrane-bound PR3 (mbPR3) at the neutrophil surface is the prime antigenic target of antineutrophil cytoplasmic autoantibodies (ANCA) in granulomatosis with polyangiitis (GPA), a vasculitis of small blood vessels and granulomatous inflammation of the upper and/or lower respiratory tracts. The interaction of ANCA with mbPR3 results in excessive activation of neutrophils to produce reactive oxygen species and liberation of granular proteinases to the pericellular environment. In this review, we focus on PR3 and dipeptidyl peptidase I as attractive pharmacological targets whose inhibition is expected to attenuate autoimmune activation of neutrophils in GPA