Haunted Heroines: An Examination of the Complication of the Gothic Heroine

This undergraduate research thesis is an examination of two of the most significant evolutions of the literary figure of the Gothic heroine, focusing on innovations made by Jane Austen in Northanger Abbey (1817) and Charlotte Brontë in Jane Eyre (1847). It discusses the origins of the Gothic heroine, set up by Horace Walpole in The Castle of Otranto (1764), and examines the ways that Austen and Brontë make their heroines more internally complex, bringing not only realism to the Gothic heroine but a psychological depth to the feminine Gothic

When the Law is Understood—L3C No

The November, 2009 issue of Community Dividend, included an article entitled “The L3C: A new business model for socially responsible investing.” The article spoke enthusiastically about “[t]he low-profit limited liability company, or L3C, …a newly developed form of business that blends attributes of nonprofit and for-profit organizations in order to promote investment in socially responsible objectives.” We understand the enthusiasm; proponents of the L3C have predicted dramatic benefits. However, after careful study of the relevant law, we have concluded that the enthusiasm is misplaced. The L3C concept is fundamentally flawed, potentially dangerous, and at best counterproductive. We also understand that our skepticism may make us seem like a pair of Grinches. We want, therefore, to briefly describe our experience in this realm of law and to outline the legal issues we have considered. We have each been involved in the law and practice of limited liability companies for more than 20 years. One of us (Bill) has a full-time practice that includes substantial amounts of work with low-income housing and community development financing transactions and extensive work with nonprofit organizations. The other of us (Daniel) is a professor of law, who was the Reporter for the Uniform Limited Partnership Act (from the National Conference of Commissioners on Uniform State Laws) and Co-Reporter for the Revised Uniform Limited Liability Company Act. Each of us has taught and written extensively about LLCs. In our assessment of the L3C concept, we have considered the arguments and claims of the L3C’s proponents (including statements made in state legislatures), and also the laws providing for limited liability companies, regulating charitable foundations, and governing the sale of securities. The promoters of state L3C legislation describe three principal benefits from the L3C form: (1) the L3C complies or “dovetails” with IRS program-related investment (“PRI”) rules, thereby enabling private foundation investment in qualifying business enterprises that operate according to for-profit metrics (but nonetheless for socially beneficial purposes); (2) the L3C permits “tranched investment” through which foundations can make high risk/low return investments to enable profit-seekers to make low risk/high return investments, thereby bringing market-rate capital into socially beneficial enterprises; and (3) the L3C creates a “brand” to enable easy comprehension and use of the PRI tool. Our research shows that none of these benefits exist

Characterization and expression analysis of the groESL operon of Bartonella bacilliformis

The groESL operon of Bartonella bacilliformis, a facultative intracellular, Gram-negative bacterium and etiologic agent of Oroya Fever, was characterized. Sequence analysis revealed an operon containing two genes of 294 (groES) and 1632 nucleotides (groEL) separated by a 55-nt intergenic spacer. The operon is preceded by a 72-nt ORF (ORF1) that encodes a hypothetical protein with homology to a portion of the HrcA repressor for groESL. A divergent fumarate hydratase C (fumC) gene lies further upstream. Deduced amino acid sequences for B. bacilliformis GroEL and GroES revealed a high degree of identity with homologues from other Bartonella and alpha-Protebacteria. A single transcriptional start site (TSS) was mapped 79 nucleotides upstream of the groES start codon, regardless of incubation temperature. The TSS was located immediately 5\u27 to a potential controlling inverted repeat of chaperonin expression (CIRCE) element and is preceded by a sigma70-like promoter. The operon is followed by a predicted rho-independent transcriptional terminator. Northern blot analysis indicated that groES and groEL are co-transcribed as a single mRNA of approximately 2.4 kb. A 6-h time course analysis by qRT-PCR showed that groEL expression increases 1.3-fold within 30 min of a temperature upshift from 30 to 37 degrees C, with maximum transcription reached after 60 min (approximately 4.3-fold), followed by a steady decrease to background (30 degrees C) transcription levels by 6 h. Western blot analysis revealed a 1.4- and 1.5-fold increase in GroEL synthesis following a temperature upshift or by inhibiting DNA supercoiling with coumermycin A1, respectively. Functional expression and complementation of temperature-sensitive Escherichia coli groES or groEL mutants with the cloned operon allowed them to grow at otherwise restrictive temperatures

An introduction to crowdsourcing for language and multimedia technology research

Language and multimedia technology research often relies on large manually constructed datasets for training or evaluation of algorithms and systems. Constructing these datasets is often expensive with significant challenges in terms of recruitment of personnel to carry out the work. Crowdsourcing methods using scalable pools of workers available on-demand offers a flexible means of rapid low-cost construction of many of these datasets to support existing research requirements and potentially promote new research initiatives that would otherwise not be possible

In situ synthesized low-PtCo@porous carbon catalyst for highly efficient hydrogen evolution

Electrochemical water splitting is a sustainable method for producing hydrogen - the ultimate clean energy carrier. However, high cost and poor stability of the Pt catalyst for hydrogen evolution reaction (HER) hinder its wide applications. Here, we report a facile approach to synthesize an ultra-low PtCo bimetallic catalyst embedded in porous carbon via direct annealing of Pt-doped ZIF-67. The resulting catalyst (CPt@ZIF-67) with only 5 wt% Pt loading exhibits better performance than commercial 20 wt% Pt/C, achieving a Tafel slope of 27.1 mV dec -1 with an overpotential of only 50 mV at current density of 10 mA cm -2 . Theoretical simulations show that carbon cages generated over the bimetal clusters during annealing dramatically reduce the free energy for HER. The free energy does not reduce proportionally with increasing Pt loading, implying the need to ensure appropriate Pt placement on surfaces, rather than simply raising Pt level, in order to enhance effectiveness of a Pt-based catalyst. The study provides a viable approach for developing cost-effective Pt-related catalysts for HER

Influence of synthesis conditions on the structure of nickel nanoparticles and their reactivity in selective asymmetric hydrogenation

Unsupported and SiO2-supported Ni nanoparticles (NPs), were synthesised via hot-injection colloidal route using oleylamine (OAm) and trioctylphosphine (TOP) as reducing and protective agents, respectively. By adopting a multi-length scale structural characterization, it was found that by changing equivalents of OAM and TOP not only the size of the nanoparticles is affected but also the Ni electronic structure. The synthetized NPs were modified with (R,R)-tartaric acid (TA) and investigated in the asymmetric hydrogenation of methyl acetoacetate to chiral methyl-3-hydroxy butyrate. The comparative analysis of structure and catalytic performance for the synthetized catalysts has enabled us to identify a Ni metallic active surface, whereby the activity increases with the size of the metallic domains. Conversely, at the high conversion obtained for the unsupported NPs no impact of particle size on the selectivity was observed. (R)-selectivity was very high only on catalysts containing positively charged Ni species such as over the SiO2-supported Ni oxide NPs. This work shows that the chiral modification of metallic Ni NPs with TA is insufficient to maintain high selectivity towards the (R)-enantiomer at long reaction time and provide guidance for the engineering of long-term stable enantioselective catalysts

Controlling the Production of Acid Catalyzed Products of Furfural Hydrogenation by Pd/TiO2

We demonstrate a modified sol-immobilization procedure using (MeOH)x/(H2O)1-x solvent mixtures to prepare Pd/TiO2 catalysts that are able to reduce the formation of acid catalyzed products, e. g. ethers, for the hydrogenation of furfural. Transmission electron microscopy found a significant increase in polyvinyl alcohol (PVA) deposition at the metal-support interface and temperature programmed reduction found a reduced uptake of hydrogen, compared to an established Pd/TiO2 preparation. We propose that the additional PVA hinders hydrogen spillover onto the TiO2 support and limits the formation of Brønsted acid sites, required to produce ethers. Elsewhere, the new preparation route was able to successfully anchor colloidal Pd to the TiO2 surface, without the need for acidification. This work demonstrates the potential for minimizing process steps as well as optimizing catalyst selectivity – both important objectives for sustainable chemistry

Quality Assessment in Crowdsourced Indigenous Language Transcription

The digital Bleek and Lloyd Collection is a rare collection that contains artwork, notebooks and dictionaries of the indigenous people of Southern Africa. The notebooks, in particular, contain stories that encode the language, culture and beliefs of these people, handwritten in now-extinct languages with a specialised notation system. Previous attempts have been made to convert the approximately 20000 pages of text to a machine-readable form using machine learning algorithms but, due to the complexity of the text, the recognition accuracy was low. In this paper, a crowdsourcing method is proposed to transcribe the manuscripts, where non-expert volunteers transcribe pages of the notebooks using an online tool. Experiments were conducted to determine the quality and consistency of transcriptions. The results show that volunteeers are able to produce reliable transcriptions of high quality. The inter-transcriber agreement is 80% for |Xam text and 95% for English text. When the |Xam text transcriptions produced by the volunteers are compared with a gold standard, the volunteers achieve an average accuracy of 64.75%, which exceeded that in previous work. Finally, the degree of transcription agreement correlates with the degree of transcription accuracy. This suggests that the quality of unseen data can be assessed based on the degree of agreement among transcribers

A single intranasal dose of chimpanzee adenovirus-vectored vaccine protects against SARS-CoV-2 infection in rhesus macaques

The deployment of a vaccine that limits transmission and disease likely will be required to end the coronavirus disease 2019 (COVID-19) pandemic. We recently described the protective activity of an intranasally administered chimpanzee adenovirus-vectored vaccine encoding a pre-fusion stabilized spike (S) protein (ChAd-SARS-CoV-2-S [chimpanzee adenovirus-severe acute respiratory syndrome-coronavirus-2-S]) in the upper and lower respiratory tracts of mice expressing the human angiotensin-converting enzyme 2 (ACE2) receptor. Here, we show the immunogenicity and protective efficacy of this vaccine in non-human primates. Rhesus macaques were immunized with ChAd-Control or ChAd-SARS-CoV-2-S and challenged 1 month later by combined intranasal and intrabronchial routes with SARS-CoV-2. A single intranasal dose of ChAd-SARS-CoV-2-S induces neutralizing antibodies and T cell responses and limits or prevents infection in the upper and lower respiratory tracts after SARS-CoV-2 challenge. As ChAd-SARS-CoV-2-S confers protection in non-human primates, it is a promising candidate for limiting SARS-CoV-2 infection and transmission in humans