Agrobacterium mediated transformation of Sri Sumrong 60, a Thai cotton variety

Techniques were developed for the induction of embryogenic callus, as well as the transformation and regeneration of Sri Sumrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants on MS based medium containing zeatin and NAA and subculturing on an hormone-free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Sumrong 60 with a synthetic crylA(b) gene was achieved by using Agrobacterium tumefaciens strain, with the addition of 100 mM acetosyringone to the medium during inoculation and co-cultivation steps. PCR amplification was used to verify the presence of ctylA(b) in regenerated plants, thus suggesting the successful transformation of the Thai cotton variety Sri Sumrong 60. (Résumé d'auteur

A new tospovirus species infecting Solanum esculentum and Capsicum annuum in Thailand

Based on distinct sequences, serology and host range, isolates of a new tospovirus species infecting field crops of tomato and pepper in Thailand are shown to be members of the provisionally named Tomato necrotic ringspot virus. © Australasian Plant thology ociety 2010