HuR biological function involves RRM3-mediated dimerization and RNA binding by all three RRMs

HuR/ELAVL1 is an RNA-binding protein involved in differentiation and stress response that acts primarily by stabilizing messenger RNA (mRNA) targets. HuR comprises three RNA recognition motifs (RRMs) where the structure and RNA binding of RRM3 and of full-length HuR remain poorly understood. Here, we report crystal structures of RRM3 free and bound to cognate RNAs. Our structural, NMR and biochemical data show that RRM3 mediates canonical RNA interactions and reveal molecular details of a dimerization interface localized on the α-helical face of RRM3. NMR and SAXS analyses indicate that the three RRMs in full-length HuR are flexibly connected in the absence of RNA, while they adopt a more compact arrangement when bound to RNA. Based on these data and crystal structures of tandem RRM1,2-RNA and our RRM3-RNA complexes, we present a structural model of RNA recognition involving all three RRM domains of full-length HuR. Mutational analysis demonstrates that RRM3 dimerization and RNA binding is required for functional activity of full-length HuR in vitro and to regulate target mRNAs levels in human cells, thus providing a fine-tuning for HuR activity in vivo.España, MINECO BFU2015-71017España, Junta de Andalucía CVI-BIO198; P11-CVI7216 to I.D.M

XPS Determination of Compositions of Alloy Surfaces and Surface Oxides on Mechanically Polished Iron-Chromium Alloys(Metallurgy)

Using the XPS technique, the compositions of the alloy surface and the surface oxide on mechanically polished iron-chromium alloys were studied. For quantitative analysis, the ratio of photo-ionization cross section of Fe 2p_ electrons to that of Cr 2p_ electrons was estimated as 1.35 for Al Kα_ radiation. Assuming a homogeneous oxide layer, the thickness of the oxide layer and compositions of the alloy surface and the surface oxide were simultaneously determined. The results showed no enrichment of chromium or iron either in the oxide layer or the alloy surface. Results on binding energies were also given

Optical to Near-IR Spectrum of a Massive Evolved Galaxy at z = 1.26

We present the optical to near-infrared (IR) spectrum of the galaxy TSPS J1329-0957, a red and bright member of the class of extremely red objects (EROs) at z = 1.26. This galaxy was found in the course of the Tokyo-Stromlo Photometry Survey (TSPS) which we are conducting in the southern sky. The spectroscopic observations were carried out with the Gemini Multi-Object Spectrograph (GMOS) and the Gemini Near Infra-Red Spectrograph (GNIRS) mounted on the Gemini-South telescope. The wide wavelength coverage of 0.6 - 2.3 um provides useful clues as to the nature of EROs while most published spectra are limited to a narrower spectral range which is dictated by the need for efficient redshift determination in a large survey. We compare our spectrum with several optical composite spectra obtained in recent large surveys, and with stellar population synthesis models. The effectiveness of using near-IR broad-band data, instead of the spectral data, in deriving the galaxy properties are also investigated. We find that TSPS J1329-0957 formed when the universe was 2 - 3 Gyr old, and subsequently evolved passively to become one of the most massive galaxies found in the z = 1 - 2 universe. Its early type and estimated stellar mass of M* = 10^{11.5} Msun clearly point to this galaxy being a direct ancestor of the brightest elliptical and spheroidal galaxies in the local universe.Comment: 18 pages, 4 figures. Accepted for publication in Ap

ESCA Study of the Passive Film on an Extremely Corrosion-Resistant Amorphous Iron Alloy

X-ray photoelectron spectroscopy was applied to study the composition of the passive film formed on an extremely corrosion resistant amorphous Fe-10at.%Cr-13at.%P-7at.%C alloy in 1 N HCl. The passive film consists mainly of hydrated chromium oxyhydroxide which is a common major constituent of passive films on crystalline stainless steels. The extremely high corrosion resistance of the amorphous alloy can only in part be attributed to the formation of a protective hydrated chromium oxyhydroxide film

Development of Thick-foil and Fine-pitch GEMs with a Laser Etching Technique

We have produced thick-foil and fine-pitch gas electron multipliers (GEMs) using a laser etching technique. To improve production yield we have employed a new material, Liquid Crystal Polymer, instead of polyimide as an insulator layer. The effective gain of the thick-foil GEM with a hole pitch of 140 um, a hole diameter of 70 um, and a thickness of 100 um reached a value of 10^4 at an applied voltage of 720 V. The measured effective gain of the thick-foil and fine-pitch GEM (80 um pitch, 40 um diameter, and 100 um thick) was similar to that of the thick-foil GEM. The gain stability was measured for the thick-foil and fine-pitch GEM, showing no significant increase or decrease as a function of elapsed time from applying the high voltage. The gain stability over 3 h of operation was about 0.5%. Gain mapping across the GEM showed a good uniformity with a standard deviation of about 4%. The distribution of hole diameters across the GEM was homogeneous with a standard deviation of about 3%. There was no clear correlation between the gain and hole diameter maps.Comment: 21 pages, 9 figure

A New Lead Chemical for Strigolactone Biosynthesis Inhibitors

Several triazole-containing chemicals have previously been shown to act as efficient inhibitors of cytochrome P450 monooxygenases. To discover a strigolactone biosynthesis inhibitor, we screened a chemical library of triazole derivatives to find chemicals that induce tiller bud outgrowth of rice seedlings. We discovered a triazole-type chemical, TIS13 [2,2-dimethyl-7-phenoxy-4-(1H-1,2,4-triazol-1-yl)heptan-3-ol], which induced outgrowth of second tiller buds of wild-type seedlings, as observed for non-treated strigolactone-deficient d10 mutant seedlings. TIS13 treatment reduced strigolactone levels in both roots and root exudates in a concentration-dependent manner. Co-application of GR24, a synthetic strigolactone, with TIS13 canceled the TIS13-induced tiller bud outgrowth. Taken together, these results indicate that TIS13 inhibits strigolactone biosynthesis in rice seedlings. We propose that TIS13 is a new lead compound for the development of specific strigolactone biosynthesis inhibitors