Investigation of the preconcentration conditions of Cd(II), Cu(II) and Pb(II) ions with solid phase extraction by using fungus (Mucor pusillus)

Bu çalışmada, Mucor pusillus immobilize edilmiş Amberlit XAD-4 üzerinde Cd(II), Cu(II) ve Pb(II) iyonlarının katı faz özütleme yöntemi ile zenginleştirilme şartları araştırıldı. Metal iyonların tayinleri yüksek çözünürlüklü alevli atomik absorpsiyon spektroskopi cihazı ile yapıldı. Çalışılan metal iyonların geri kazanma verimine örnek çözeltisinin ortamının pH'sının, geri alma çözeltilerinin türü ve derişimlerinin, çözelti akış hızının ve çözelti hacminin etkisi incelendi. Belirlenen en uygun deneysel şartlarda Cd(II), Cu(II) ve Pb(II) iyonlarının geri kazanma verimi %95 güven seviyesinde sırasıyla, %99±3, %97±2 ve %96±2 olarak, bulundu. Yöntemin kesinliği, çalışılan iyonlar için gözlenebilme sınırı, bilinen miktarda analat eklenerek ve standart referans maddeler kullanılarak yöntemin doğruluğu belirlendi. Geliştirilen yöntem çeşme suyu, Kızılırmak suyu ve domates yaprağı örneklerinde, çalışılan metal iyonların tayinine uygulandı. Gerçek örnekler için bağıl standart sapma ve bağıl hata değerleri en fazla %8 olarak bulundu.In this study, preconcentration conditions of Cd(II), Cu(II) and Pb(II) ions for Mucor pusillus immobilized on Amberlite XAD-4 by solid phase extraction method were investigated. Mucor pusillus was studied as microorganism. The determination of the elements was carried out by flame atomic absorption spectrometry. The effect of pH, flow rate and volume of sample solution on the recovery of the studied ions was investigated. The recoveries Cd(II), Cu(II) and Pb(II) ions were found as 99± 3%, 97±2 % and 96±2 % respectively for Mucor pusilus immobilized on Amberlite XAD-4, under the optimum conditions determined. The accuracy of the method by using addition samples, precision of the method and limit of detection for the studied ions were determined. The proposed method was applied to tap water, Kızılırmak river water and tomato leaves for the determination of the analytes. Relative standard deviation and relative error were found as about 8 %, respectively for real samples

Investigation of the preconcentration conditions of Co(II), Cr(III) and Ni(II) ions with solid phase extraction by using fungus (Mucor Pusillus) immobilized on activated aluminum oxid

Bu çalışmada, Mucor pusillus immobilize edilmiş aktif alüminyum oksit üzerinde Co(II), Cr(III) ve Ni(II) iyonlarının katı faz özütleme yöntemi ile zenginleştirilme şartları araştırıldı. Metal iyonlarının tayinleri yüksek çözünürlüklü alevli atomik absorpsiyon spektroskopi cihazı ile yapıldı. Çalışılan metal iyonların geri kazanma verimine örnek çözeltisinin ortamının pH'sının, elüent çözeltilerinin türü ve derişimi, çözelti akış hızının ve çözelti hacminin etkisi incelendi. Belirlenen en uygun deneysel şartlarda Co(II), Cr(III) ve Ni(II) iyonlarının geri kazanma verimi %95 güven seviyesinde sırasıyla, %99±3, %97±2 ve %98±2 olarak bulundu. Yöntemin kesinliği, çalışılan iyonlar için gözlenebilme sınırı, bilinen miktarda metal iyonları eklenerek ve standart referans maddeler kullanılarak yöntemin doğruluğu belirlendi. Geliştirilen yöntem çeşme suyu, Kızılırmak suyu ve domates yaprağı örneklerinde, çalışılan metal iyonların tayinine uygulandı. Gerçek örnekler için bağıl standart sapma ve bağıl hata değerleri en fazla % 8 olarak bulundu.In this study, preconcentration conditions of Co(II), Cr(III) and Ni(II) ions for Mucor pusillus immobilized on activated Al2O3 by solid phase extraction method were investigated. Mucor pusillus was studied as microorganism. The determination of the trace metal ions was carried out by flame atomic absorption spectrometry. The effect of pH, flow rate and volume of sample solution on the recovery of the studied ions was investigated. The recoveries of Co(II), Cr(III) and Ni(II) ions were found as 99±3%, 97±2%, and 98±2%, respectively for Mucor pusillus immobilized on activated Al2O3, under the optimum conditions. The accuracy of the method by using addition samples, precision of the method and limit of detection for the studied ions were determined. The proposed method was applied to tap water, Kızılırmak river water and tomato leaves for the determination of the analytes. Relative standard deviation and relative error were found as about 8%, respectively for real samples

Mucor pusillus immobilized Amberlite XAD-4 biocomposites for preconcentration of heavy metal ions by solid-phase extraction method

Abstract Background Solid phase extraction has been an effective tool for the determination of metal ions at trace or sub trace level from environmental aquatic streams. Sensitivity, accuracy, versatility and reusability of adsorbent entitle the solid phase as effective technique for the determination of metal ions. Methods A solid phase extraction procedure has been described for the determination of Cd, Cu, and Pb by High Resolution–Continuum Source Flame Atomic Absorption Spectrometry HR-CS FAAS using a mini-column of Mucor pusillus (Lindt., 1886) immobilized on Amberlite XAD-4. Method has been optimized by changing the pH of analyte solution, solid phase dosage, volume of eluents, flow rate of sample solution and volume of the sample solutions. Results The recoveries of Cd, Cu, and Pb under the optimum conditions were 99±3%, 97±2% and 96±2%, respectively. The resulting preconcentration procedure ensured a 50-fold improvement in the sensitivity of the elements. The detections limits were 62, 74 and 235 ng/mL for Cd, Cu, and Pb before enrichment, respectively. The method was validated by analysis of tomato leaves reference materials (SRM 1573a). Conclusions The proposed enrichment method has been successfully applied for the determination of Cd, Cu, and Pb in tomato leaves and water samples with a relative error ≤8%. This method is simple, sensitive, and accurate especially for water sample, only 200 mg of sorbent are required to capture the analytes. It can be concluded that the use of Mucor pusillus (Lindt., 1886) enhanced the sorption ability of Amberlite XAD-4 resin for the retention of Cd, Cu, and Pb