EBV infection increases with disease severity.

<p>(A–B) EBER-ISH staining was performed on paired periodontal pocket DS and SS samples (n = 40) from CP patients (n = 20), and from HS samples from healthy gingival sulcus from HDs (n = 10). Two representative fields (x40) of EBER staining (EBER) is shown for (A) one selected CP patient and (B) for one selected HD. Negative controls (NEG) were processed using a random PNA probe, and for each individual, the same cell sampling was used for positive and negative staining. (C) EBER-ISH-based determination of the frequency of EBV-infected pECs (EBER^pos) in periodontal samples from healthy sites (HS), swallow sites (SS), and deep sites (DS). The graph (left part) shows the tendency curve of EBER^pos pECs and the clinical attachment level (CAL) in 40 paired-samples from 20 CP patients and 10 samples from healthy donors (same as in A and B). The dot-plot analysis (right part) shows the comparative analysis of the frequency of EBER^pos pECs in paired-samples (SS circles, DS triangles) collected from 20 CP patients (same samples as on graft). (D) The average levels of EBNA1 transcript were also compared between whole RNA from HS (n = 10) and 12 paired-samples (SS and DS, n = 6 for each) from 6 CP patients (same samples as in C). The 36B4 housekeeping gene was used for normalization, with results presented as relative to 36B4 using a LOG₁₀ scale. The p values were calculated using Wilcoxon signed-rank test.</p

EBER-ISH staining of liquid-based periodontal samples reveals EBV-Infected epithelial cells.

<p>(A) Representative MGG staining of cytospin cells collected from PP samples (n = 10). Large spread epithelial-like cells (EC), polymorphonuclear leucocytes (PMN), mononuclear cells (MNC) as well as traces of dental plaque (DP) are indicated with arrows. Size bar represents 15 µM. (B) Representative CK19 staining of pECs from 5 CP patients. Coverslips were processed for IHC with DAB chromogen staining, CK19 specific staining was assessed by comparing with background staining observed using non specific mouse IgGs (not shown). Size bar represents 15 µM. (C) Nuclear EBER-ISH staining in pECs and palECs. EBER-ISH was used to detect EBER in periodontal and palatal cells sampled from 3 patients with chronic periodontitis. Two representative fields (x20) of EBER staining (EBER) are shown for the same selected CP patient with pECs (left panel) and palECs (right panel).</p

LMP1 and LMP2 IF co-staining in oral epithelial cells.

<p>(A–D) Detection of LMP2 and LMP1 by IF double staining in oral epithelial cells, LMP2 (green, upper left), LMP1 (red, upper right), DAPI (blue, below left), merge (below right). (A) Oral TR146 ECs infected with Ad₅F₃₅ recombinant adenoviruses expressing LMP2 and/or ΔLMP1 were used to assess IF-based co-staining of LMP1 and LMP2 in oral epithelial cells. Results show representative specific co-detection of LMP1 and LPM2 in TR146. (B–D) pECs and palECs were sampled from 3 patients with CP and results are shown for the same selected patient. (B, C) Representative LMP1 and LMP2 co-staining in pECs. (D) Lack of LMP1 and LMP2 specific staining in palECs. TR146 were handled and fixed in a manner similar to pECs and palECs. Processing of all cell preparation with two different rat LMP2-specific antibodies (clone 14B7 clone 15F9) showed very similar results, only results obtained with clone 15F9 are presented. Specific staining was assessed by comparing with LMP1 and LMP2 negative ECs, i.e., TR146 cells infected with adenoviral vectors expressing either inactive-LMP1 (Ad₅F₃₅-ΔLMP1) or LMP2 (Ad₅F₃₅-LMP2) (not presented), and EBV-negative palECs. Background staining was also assessed in presence of unspecific rat primary antibodies (not shown). Size-bar is indicated for each panel.</p

Specific real-time RT-PCR quantification of EBV gene expression in periodontal material.

<p>Whole RNA was isolated from 12 periodontal paired-samples (PP) from 6 CP patients, 3 palatal sites from 3 additional CP patients (pal), 4 EBV-infected cell lines (B95-8, L591, LCL1, C666-1) and an EBV-negative cell line (HepG2). The average levels of EBNA1, EBNA2, LMP1, LMP2, and BZLF1 EBV transcripts in clinical samples (shown as mean and SD) were compared with the expression of these EBV-genes in the different cell lines. Values obtained with an EBV-negative cell line were used to determine background level (HDLM2). The average levels of the B-cell marker CD20 transcript were also compared between LCL1, pal, and PP samples. The 36B4 housekeeping gene was used for normalization, with results presented as relative to 36B4 using a LOG₁₀ scale. The results shown are from one representative experiment of two RT-PCR experiments performed, each in triplicate.</p

Infection of periodontal epithelial cells by EBV is associated with apoptotic cell death.

<p>TUNEL assay associated with IF LMP2 detection was used to identify apoptotic cells and EBV-infected cells in DS samples from 5 CP patients. (A) Representative double fluorescent staining with antibodies specific for LMP2 (upper right panel), TUNEL staining (below left panel), with the latter two merged (below right panel). The upper-left panel shows DAPI staining of nuclei in the same field (x20). (B) Quantitative evaluations of A. Cell counting of LMP2^pos (LMP2), TUNEL^pos (TUNEL) and double positive (LMP2 & TUNEL) pECs (n = 5). Data represent the frequency of positive pECs identified in each category. (C) Calculations from B showing the frequency of apoptotic pECs (TUNEL^pos) among EBV-infected pECs (LMP2) (left part) and the frequency of EBV-infected pECs (LMP2^pos) among apoptotic pECs (TUNEL) (right part). Mean and standard deviation is shown for each group.</p

Healthy donors and sample information.^a

a<p>Characteristics for the healthy donors (HDs) used in this study (n = 10).</p>b<p>For each patient, one sampling was performed in healthy gingival sulcus of the right mandibular molar. Normal Clinical attachment level and gingival index values are considered to be less than 3 mm and 1, respectively.</p>c<p>Frequency (%) of EBV-infected periodontal epithelial cells (pECs).</p>d<p>RNA samples used for EBNA1 and CCL20 gene expression analysis.</p>e<p>Overall mean values and standard deviations (SD).</p

Production of the inflammatory chemokine CCL20 by EBV-infected periodontal epithelial cells.

<p>(A) Left panel shows representative CCL20 staining of pECs from 5 CP patients (DS samples) (HIS, x40) of EBV-infected cells (EBER-ISH) (solid arrows) and of EBV-negative pECs (dotted arrows). Right panel shows background staining observed using nonspecific goat IgGs. (B) Quantitation of (A) (n = 5). The frequency (%) of CCL20^pos pECs among EBV-infected pECs (EBER, left part) and of EBER^pos pECs among CCL20-producing pECs (CCL20, right part) is shown. Mean and standard deviations are shown for each group. (C) Real-time RT-PCR quantification of CCL20 transcripts in whole RNA isolated from 12-paired RNA samples from 6 CP patients (SS n = 6, DS n = 6) and 2 RNA samples from healthy donors. (D) Real-time RT-PCR quantification of EBNA1 and CCL20 transcripts in whole RNA isolated from CP patients (n = 8) and from healthy donors (n = 2). Graph shows the linear tendency curve of CCL20 related to EBNA1. Simple linear regression analysis showed a positive correlation. Data are representative of 2 independent experiments, each performed in triplicate. The 36B4 housekeeping gene was used for normalization. The results are presented as relative to 36B4 (×10⁻⁶).</p

Patients and sample information.^a

a<p>Characteristics for the main CP patient cohort used in this study (n = 20).</p>b<p>For each patient, paired-sampling was performed, and upper and lower values shown correspond to shallow and deep periodontal sites (SS and DS), respectively. Normal clinical attachment level and gingival index values are considered to be less than 3 mm and 1, respectively.</p>c<p>Frequency (%) of EBV-infected periodontal epithelial cells (pECs). The mean frequency (and standard deviation) of EBV-infected cells was 18.5% (+/−5.12) for SS, and 26.45% (+/−6.8) for DS.</p>d<p>RNA samples used for EBV and CCL20 gene expression analysis.</p>e<p>Samples used for TUNEL experiments.</p>f<p>Overall mean values and standard deviations (SD).</p