Anticancer effect of a novel palladium-saccharinate complex of terpyridine by inducing apoptosis on ehrlich ascites carcinoma (EAC) in balb-C mice

Background/Aim: [Pd(sac)(terpy)](sac)•4H2O (sac=saccharinate and terpy=2,2′:6′,2-terpyridine) is newlysynthesized palladium(II) (Pd) complex. We investigated the antiproliferative and apoptotic effects of this complex on Ehrlich ascites carcinoma (EAC). Materials and Methods: EAC cells were administered to 33 Balb/c mice. Mice were divided randomly into four groups: control, cisplatin, Pd(II) complex and paclitaxel. Control group animals received 0.9% NaCl; other groups received treatments cisplatin, Pd(II) complex and paclitaxel on days 7 and 12. At day 14, animals were sacrificed. Expression of active caspase-3, p53 and proliferating cell nuclear antigen (PCNA) was investigated and apoptosis was evaluated by terminal deoxynucleotidyltransferase (TdT)-mediated nick-end labelling (TUNEL) technique. Results: Expression of p53 and PCNA were found to be decreased (p<0.0001), cells with active caspase-3 and TUNEL-positive cells were found to be increased (p<0.0001) in all treatment groups. Conclusion: Like cisplatin and paclitaxel, this Pd(II) complex has a strong anticancer activity against EAC by inducing apoptosis and suppressing proliferation in vivo.Istanbul University (UDP-47082

Ovarian Granulosa Cell Tumor and Complex Mammary Adenocarcinoma in a Bitch.

An 11-year old boxer bitch was presented to the Department of Obstetrics and Gynaecology clinic for routine ovariohysterectomy and coincidentally an ovarial mass was detected which was diagnosed as granulosa cell tumor (GCT) in pathologic examinations. Blood sample was collected for hormonal analysis during this surgery in which estrogen concentration was under the reference range, progesterone concentration was normal. Mammary gland enlargements were also detected on the dog during initial examination with no other sign regarding to vulvar discharge/swelling. One month later following ovariohysterectomy, the dog was presented again for bilateral total mastectomy. Tumoral tissues were diagnosed as complex adenocarcinoma grade II, histopathologically. The possible interaction and promotional effect of GCT on the development of mammary tumors (MTs) is not yet fully understood. Regarding to serum estrogen and progesterone concentrations were not above the reference ranges suggest us GCT did not secrete hormones yet. In conclusion, this case is important as it reports the possible effect of GCT on the development of MTs and suggests not to ignore this disease in a dog with mammary tumours