PHYSICO-CHEMICAL, PHYTOCHEMICAL AND HPTLC ANALYSIS OF THE WHOLE PLANT OF ORTHOSIPHON THYMIFLORUS (ROTH.) SLEESEN

 Objective: The plant Orthosiphon thymiflorus (Roth.) sleesen is an herb belonging to the family Lamiaceae. It has been reported to possessseveral pharmacological properties. The study was aimed at carrying out the physico-chemical, phytochemical and high-performance thin layerchromatography (HPTLC) analysis on the whole plant of O. thymiflorus.Methods: The physico-chemical parameters were tested using standard methods. The preliminary tests for phytochemicals present were testedusing conventional methods. CAMAG HPTLC system equipped with TLC autosampler 4 applicator, TLC scanner 3 and win CATS 1.4.4. software wasused for HPTLC analysis of the plant. The different extracts were developed using suitable mobile phases using standard procedures and scannedunder ultraviolet 254 nm. The plates were also viewed under 366 nm and after derivatization with vanillin-sulfuric acid.Results: The physico-chemical parameters were studied. The plant was found to be free from adulteration and contamination. On phytochemicalanalysis, the plant showed the presence of triterpenoid, flavonoid, alkaloid, phenol, steroid, sugars, quinone and coumarin in different extracts tested.The TLC plate showed several spots at different Rf when viewed under 254 nm, 366 nm and after derivatization with vanillin-sulfuric acid. The HPTLCprofile showed several peaks that indicated the presence of various phytochemicals present in the plant.Conclusion: The study will help in standardization of the plant. The HPTLC profile will help in authentication and standardization of the plant.Keywords: Orthosiphon thymiflorus, High performance thin layer chromatography, Phytochemical screening, Standardization

<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Analytical standards on <i>Meka Natak Kulikai</i></span>

325-330Meka Natak Kulikai (MNK) is a classical Siddha compound formulation. This drug is prescribed for treating kunmam (peptic ulcer), kulir curankal (fever with rigor), vatam (rheumatism), pitta nir (excessive bile secretion), udal veluppu (anaemia), enpurukki noy (tuberculosis), vayiruvali (abdominal colic pain/abdominal pain), ceriyamai (indigestion), and malakkattu (constipation). Considering the importance of the uses of the drug, it was proposed to evolve the standard operating procedure for its manufacturing process and pharmacopoeial standards. It is mentioned in SFI that mattirai retains the potency for one year if stored in a clean, dry, air tight container. Hence, it was proposed to study its shelf life as well. For the above study, one laboratory scale and three batches of industrial scale preparations were done and analyzed for the same parameters. The developed method and standard would be useful for manufacturers and pharmacies to prepare formulation with uniform consistency. The result will also benefit the researcher in testing the preparation quantitatively