Pharmacokinetic Parameters Determination of Gendarusin a in Men Subject Urine After Administration of Ethanol Extract of Justicia Gendarussa Burm. F. Leaf (Ethno Medicine Research)

Justicia gendarussa Burm. f (Famili: Acanthaceae) has flavonoid that inhibits hyaluronidase enzyme of spermatozoa in the fertilization process. Previous research reported that the major component of J.gendarussa was 6,8-di-L-arabinopyranosil- 4’,5,7-trihydroxy flavones or 6,8-diarabinosylapigenin (gendarusin A).Objective: This experimental study has been carried out to determine the pharmacokinetic parameters of gendarusin A in human urine after single oral administration of J.gendarussa extract. Methods: Six healthy men were enlisted in this study. Urine samples were collected at intervals for 24 hours before and after six healthy volunteers administrated orally 100 ml ethanol extract suspension of J. gendarussa leaves, containing 1 g of extract equal to 16,4 mg gendarusin A, for the measurement of gendarusin A by HPLC. Result: The calibration curve of gendarusin A peak areas (y) against the concentrations (x, μg/ml) in urine was linear and the regression equations was y = 34.3496x + 63.6315 (r = 0.9992). The lowest absolute recovery was 106.10 %, while the lowest assay recovery was 81.92 %, which revealed that the accuracy of the method was satisfied. All values of the R.S.D. of intra-day precision were less than 8,12 %. The LOD and LOQ of assaying gendarusin A in urine was 0.0817 μg/ml and 0.2724 μg/ml, respectively. Conclusion: Following oral administration of J.gendarussa extract suspension, the result show that the elimination half-lives (t ½) for gendarusin A in the urinary excretion were 2,44 – 8,53 hours (mean 4,44 ± 2,14 hours) and the rates constant of elimination (Kel) were 0,08 – 0,28 hour-1 (mean 0,18 ± 0,07 hour-1)