Synthèse d'un inhibiteur du lysozyme utilisable en chromatographie d'affinité

L'objectif de ce travail est l'obtention, par hydrolyse de la chitine, de substrats du lysozyme de blanc d'oeuf de poule et la synthèse d'un nouvel inhibiteur de cette enzyme. Deux méthodes d'acétolyse de la chitine, une hydrolyse acide et une hydrolyse enzymatique ont été utilisées. La synthèse organique de l'inhibiteur est réalisée à partir du chitobiose et du p-thiophénylamine. L'inhibiteur sélectif, le di-N-acétyl p-aminophényl 1-thiochitobioside, est obtenu avec un excellent rendement. Ce composé est greffé sur le sépharose-4B activé via la carbodiimide. La chromatographie d'affinité sur ce gel montre une absorption sélective de l'enzyme. Cette protéine est désorbée avec un rendement de 70%

Lipids metabolites and essential oil from the green alga Chara vulgaris

Les stérols, les acides gras et les huiles essentielles ont été analysés chez l’algue d’eau douce Chara vulgaris. Les stérols majeurs sont le 24-éthylcholest-5-èn-3-ol et l’isofucostérol alors que les principaux acides gras sont le C16:0, le C18:0 et le C18:1. L’huile essentielle obtenue est analysée par CPG-SM. Les produits volatiles contiennent des hydrocarbures, des esters, des aldéhydes et des alcools. Le n-heptadécane, le 7-heptadécène, le phytol, le 6,10,14-triméthylpentadécan-2-one et le méthylundécanoate sont les composés volatiles les plus abondants. Deux terpénoides ont été identifiés, la 5,6-époxy-ß-ionone et la ß- ionone. Les résultats obtenus peuvent être utilisés comme critères chemo-taxonomiques

Microalgae Lipid Characterization

To meet the growing interest of utilizing microalgae biomass in the production of biofuels and nutraceutical and pharmaceutical lipids, we need suitable analytical methods and a comprehensive database for their lipid components. The objective of the present work was to demonstrate methodology and provide data on fatty acid composition, lipid class content and composition, characteristics of the unsaponifiables, and type of chlorophylls of five microalgae. Microalgae lipids were fractionated into TAG, FFA, and polar lipids using TLC, and the composition of fatty acids in total lipids and in each lipid class, hydrocarbons, and sterols were determined by GC-MS. Glyco- and phospholipids were profiled by LC/ESI-MS. Chlorophylls and their related metabolites were qualified by LC/APCI-MS. The melting and crystallization profiles of microalgae total lipids and their esters were analyzed by DSC to evaluate their potential biofuel applications. Significant differences and complexities of lipid composition among the algae tested were observed. The compositional information is valuable for strain selection, downstream biomass fractionation, and utilization

Microalgae Lipid Characterization

To meet the growing interest of utilizing microalgae biomass in the production of biofuels and nutraceutical and pharmaceutical lipids, we need suitable analytical methods and a comprehensive database for their lipid components. The objective of the present work was to demonstrate methodology and provide data on fatty acid composition, lipid class content and composition, characteristics of the unsaponifiables, and type of chlorophylls of five microalgae. Microalgae lipids were fractionated into TAG, FFA, and polar lipids using TLC, and the composition of fatty acids in total lipids and in each lipid class, hydrocarbons, and sterols were determined by GC-MS. Glyco- and phospholipids were profiled by LC/ESI-MS. Chlorophylls and their related metabolites were qualified by LC/APCI-MS. The melting and crystallization profiles of microalgae total lipids and their esters were analyzed by DSC to evaluate their potential biofuel applications. Significant differences and complexities of lipid composition among the algae tested were observed. The compositional information is valuable for strain selection, downstream biomass fractionation, and utilization.Fil: Yao, Linxing. Iowa State University; Estados UnidosFil: Gerde, Jose Arnaldo. Iowa State University; Estados Unidos. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Produccion Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Lee, Show Ling. Iowa State University; Estados UnidosFil: Wang, Tong. Iowa State University; Estados UnidosFil: Harrata, Kamel A.. Iowa State University; Estados Unido