1,377 research outputs found
Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement
<p>Abstract</p> <p>Background</p> <p><it>Pichia pastoris </it>has been recognized as an effective host for recombinant protein production. A number of studies have been reported for improving this expression system. However, its physiology and cellular metabolism still remained largely uncharacterized. Thus, it is highly desirable to establish a systems biotechnological framework, in which a comprehensive <it>in silico </it>model of <it>P. pastoris </it>can be employed together with high throughput experimental data analysis, for better understanding of the methylotrophic yeast's metabolism.</p> <p>Results</p> <p>A fully compartmentalized metabolic model of <it>P. pastoris </it>(<it>iPP</it>668), composed of 1,361 reactions and 1,177 metabolites, was reconstructed based on its genome annotation and biochemical information. The constraints-based flux analysis was then used to predict achievable growth rate which is consistent with the cellular phenotype of <it>P. pastoris </it>observed during chemostat experiments. Subsequent <it>in silico </it>analysis further explored the effect of various carbon sources on cell growth, revealing sorbitol as a promising candidate for culturing recombinant <it>P. pastoris </it>strains producing heterologous proteins. Interestingly, methanol consumption yields a high regeneration rate of reducing equivalents which is substantial for the synthesis of valuable pharmaceutical precursors. Hence, as a case study, we examined the applicability of <it>P. pastoris </it>system to whole-cell biotransformation and also identified relevant metabolic engineering targets that have been experimentally verified.</p> <p>Conclusion</p> <p>The genome-scale metabolic model characterizes the cellular physiology of <it>P. pastoris</it>, thus allowing us to gain valuable insights into the metabolism of methylotrophic yeast and devise possible strategies for strain improvement through <it>in silico </it>simulations. This computational approach, combined with synthetic biology techniques, potentially forms a basis for rational analysis and design of <it>P. pastoris </it>metabolic network to enhance humanized glycoprotein production.</p
Magnetic Catalysis and Quantum Hall Ferromagnetism in Weakly Coupled Graphene
We study the realization in a model of graphene of the phenomenon whereby the
tendency of gauge-field mediated interactions to break chiral symmetry
spontaneously is greatly enhanced in an external magnetic field. We prove that,
in the weak coupling limit, and where the electron-electron interaction
satisfies certain mild conditions, the ground state of charge neutral graphene
in an external magnetic field is a quantum Hall ferromagnet which spontaneously
breaks the emergent U(4) symmetry to U(2)XU(2).
We argue that, due to a residual CP symmetry, the quantum Hall ferromagnet
order parameter is given exactly by the leading order in perturbation theory.
On the other hand, the chiral condensate which is the order parameter for
chiral symmetry breaking generically obtains contributions at all orders. We
compute the leading correction to the chiral condensate. We argue that the
ensuing fermion spectrum resembles that of massive fermions with a vanishing
U(4)-valued chemical potential. We discuss the realization of parity and charge
conjugation symmetries and argue that, in the context of our model, the charge
neutral quantum Hall state in graphene is a bulk insulator, with vanishing
longitudinal conductivity due to a charge gap and Hall conductivity vanishing
due to a residual discrete particle-hole symmetry.Comment: 35 page
Quantum interference and Klein tunneling in graphene heterojunctions
The observation of quantum conductance oscillations in mesoscopic systems has
traditionally required the confinement of the carriers to a phase space of
reduced dimensionality. While electron optics such as lensing and focusing have
been demonstrated experimentally, building a collimated electron interferometer
in two unconfined dimensions has remained a challenge due to the difficulty of
creating electrostatic barriers that are sharp on the order of the electron
wavelength. Here, we report the observation of conductance oscillations in
extremely narrow graphene heterostructures where a resonant cavity is formed
between two electrostatically created bipolar junctions. Analysis of the
oscillations confirms that p-n junctions have a collimating effect on
ballistically transmitted carriers. The phase shift observed in the conductance
fringes at low magnetic fields is a signature of the perfect transmission of
carriers normally incident on the junctions and thus constitutes a direct
experimental observation of ``Klein Tunneling.''Comment: 13 pages and 6 figures including supplementary information. The paper
has been modified in light of new theoretical results available at
arXiv:0808.048
Controlling ferromagnetic easy axis in a layered MoS2 single crystal
We report the effective methods to induce weak ferromagnetism in pristine MoS2 persisting up to room temperature with the improved transport property, which would lead to new spintronics devices. The hydrogenation of MoS2 by heating at 300 degrees C for 1 h leads to the easy axis out of plane, while the irradiation of proton with a dose of 1 x 10(13) P/cm(2) leads to the easy axis in plane. The theoretical modeling supports such magnetic easy axes.open116160Nsciescopu
Holographic Aspects of Two-charged Dilatonic Black Hole in AdS5
We study certain features of a strongly coupled theory whose gravitational
dual is given by two-charge dilatonic black hole in AdS5 which has recently
been used to study holographic Fermi liquids. By making use of the gravity
description, we have studied conductivity, holographic entanglement entropy and
dynamics of a charged scalar field. In particular at low energy we find that
the temperature dependence of the real part of the conductivity goes as T^3 and
the background is stable against scalar condensations.Comment: 20 pages, 5 figures, V2: Refs. adde
N-type graphene induced by dissociative H-2 adsorption at room temperature
Studies of the interaction between hydrogen and graphene have been increasingly required due to the indispensable modulation of the electronic structure of graphene for device applications and the possibility of using graphene as a hydrogen storage material. Here, we report on the behaviour of molecular hydrogen on graphene using the gate voltage-dependent resistance of single-, bi-, and multi-layer graphene sheets as a function of H-2 gas pressure up to 24 bar from 300 K to 345 K. Upon H-2 exposure, the charge neutrality point shifts toward the negative gate voltage region, indicating n-type doping, and distinct Raman signature changes, increases in the interlayer distance of multi-layer graphene, and a decrease in the d-spacing occur, as determined by TEM. These results demonstrate the occurrence of dissociative H-2 adsorption due to the existence of vacancy defects on graphene.open12
Holographic c-theorems in arbitrary dimensions
We re-examine holographic versions of the c-theorem and entanglement entropy
in the context of higher curvature gravity and the AdS/CFT correspondence. We
select the gravity theories by tuning the gravitational couplings to eliminate
non-unitary operators in the boundary theory and demonstrate that all of these
theories obey a holographic c-theorem. In cases where the dual CFT is
even-dimensional, we show that the quantity that flows is the central charge
associated with the A-type trace anomaly. Here, unlike in conventional
holographic constructions with Einstein gravity, we are able to distinguish
this quantity from other central charges or the leading coefficient in the
entropy density of a thermal bath. In general, we are also able to identify
this quantity with the coefficient of a universal contribution to the
entanglement entropy in a particular construction. Our results suggest that
these coefficients appearing in entanglement entropy play the role of central
charges in odd-dimensional CFT's. We conjecture a new c-theorem on the space of
odd-dimensional field theories, which extends Cardy's proposal for even
dimensions. Beyond holography, we were able to show that for any
even-dimensional CFT, the universal coefficient appearing the entanglement
entropy which we calculate is precisely the A-type central charge.Comment: 62 pages, 4 figures, few typo's correcte
Catheter-associated bacteremia by Mycobacterium senegalense in Korea
BACKGROUND: Rapidly growing mycobacteria is recognized as one of the causative agents of catheter-related infections, especially in immunocompromised hosts. To date, however, Mycobacterium senegalense, which was known as the principal pathogen of bovine farcy, has not been reported in human infection. CASE PRESENTATION: We describe the first case of human infection by M. senegalense, which has caused catheter-related bloodstream infection in a cancer patient in Korea. The microorganism was identified by the 16S rRNA gene, rpoB, and 16S-23S rRNA gene internal transcribed spacer (ITS) sequence analyses. CONCLUSION: Our first report of catheter-associated bacteremia caused by M. senegalense suggests the zoonotic nature of this species and indicates the expansion of mycobacterial species relating to human infection. M. senegalense should be considered as one of the causes of human infections in the clinical practice
Preparation and characterization of polypyrrole/graphene nanocomposite films and their electrochemical performance
A one-step electrochemical process had been employed to synthesize nanocomposite films of polypyrrole/graphene (PPy/GR) by electrochemical polymerisation on indium tin oxide (ITO) from an aqueous solution containing pyrrole monomer, graphene oxide (GO) nanosheets and sodium p-toluenesulfonate (NapTS). The X-ray diffraction (XRD) patterns showed that the typical peak of GO at 9.9o was missing from the nanocomposite’s diffraction pattern, suggesting that the GO had been stripped off of its oxygenous groups after the reaction. We postulated that a nanocomposite film was produced through a layer-by-layer deposition based on field emission scanning electron microscope (FESEM) images. The Raman spectroscopy profiles exhibited that the D/G intensity ratio (ID/IG) of PPy was not altered by the inclusion of GO due to the low concentration of the material used. However, the concentration was sufficient to increase the specific capacitance of the nanocomposite by 20 times compared to that of pure PPy, reflecting a synergistic effect between PPy and GR, as analysed by a three-electrode electrochemical cell. The electrochemical performance of the nanocomposites was affected by varying the deposition parameters such as concentrations of pyrrole and GO, scan rate, deposition time and deposition potential
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