72 research outputs found
αC including a novel three- component non-heme diiron monooxygenase system
Please click Additional Files below to see the full abstrac
Polar Antiferromagnets Produced with Orbital-Order
Polar magnetic states are realized in pseudocubic manganite thin films
fabricated on high-index substrates, in which a Jahn-Teller (JT) distortion
remains an active variable. Several types of orbital-orders were found to
develop large optical second harmonic generation, signaling
broken-inversion-symmetry distinct from their bulk forms and films on (100)
substrates. The observed symmetry-lifting and first-principles calculation both
indicate that the modified JT q2 mode drives Mn-site off-centering upon orbital
order, leading to the possible cooperation of "Mn-site polarization" and
magnetism.Comment: 5 pages, 4 figure
Development of P450-BM3 using molecular dynamics simulations- A tribute to the late Professor Hideaki Yamada
Please click Additional Files below to see the full abstract
A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes
地球外有機化合物に対する微生物代謝の解明から全く新規な酵素系を発見 --生命分子進化の理解や産業応用に期待--. 京都大学プレスリリース. 2021-01-20.The high-valent iron-oxo species formed in the non-heme diiron enzymes have high oxidative reactivity and catalyze difficult chemical reactions. Although the hydroxylation of inert methyl groups is an industrially promising reaction, utilizing non-heme diiron enzymes as such a biocatalyst has been difficult. Here we show a three-component monooxygenase system for the selective terminal hydroxylation of α-aminoisobutyric acid (Aib) into α-methyl-D-serine. It consists of the hydroxylase component, AibH1H2, and the electron transfer component. Aib hydroxylation is the initial step of Aib catabolism in Rhodococcus wratislaviensis C31-06, which has been fully elucidated through a proteome analysis. The crystal structure analysis revealed that AibH1H2 forms a heterotetramer of two amidohydrolase superfamily proteins, of which AibHm2 is a non-heme diiron protein and functions as a catalytic subunit. The Aib monooxygenase was demonstrated to be a promising biocatalyst that is suitable for bioprocesses in which the inert C–H bond in methyl groups need to be activated
Clinical efficacy of liver resection after downsizing systemic chemotherapy for initially unresectable liver metastases
High visceral fat with low subcutaneous fat accumulation as a determinant of atherosclerosis in patients with type 2 diabetes
Insulin Treatment Attenuates Decline of Muscle Mass in Japanese Patients with Type 2 Diabetes
- …