1 research outputs found
Host–Guest Tethered DNA Transducer: ATP Fueled Release of a Protein Inhibitor from Cucurbit[7]uril
Host–guest
complexes are emerging as powerful components
in functional systems with applications ranging from materials to
biomedicine. In particular, CB7 based host–guest complexes
have received much attention for the controlled release of drugs due
to the remarkable ability of CB7 toward binding input molecules in
water with high affinity leading to displacement of CB7 from included
pharmacophores (or from drug loaded porous particles). However, the
release of bound guests from CB7 in response to endogenous biological
molecules remains limited since the input biomolecule needs to have
the appropriate chemical structure to bind tightly into the CB7 cavity.
Herein we describe a synthetic transducer based on self-assembling
DNA–small molecule chimeras (DCs) that is capable of converting
a chosen biological input, adenosine triphosphate (ATP; that does
not directly bind to the CB7 host), into functional displacement of
a protein inhibitor that is bound within the CB7 host. Our systemî—¸which
features the first example of a covalent CB-DNA conjugateî—¸is
highly modular and can be adapted to enable responsiveness to other
biologically/clinically relevant stimuli via its split DNA aptamer
architecture