Discovery of a Siderophore Export System Essential for Virulence of <em>Mycobacterium tuberculosis</em>

<div><p>Iron is an essential nutrient for most bacterial pathogens, but is restricted by the host immune system. <em>Mycobacterium tuberculosis</em> (<em>Mtb</em>) utilizes two classes of small molecules, mycobactins and carboxymycobactins, to capture iron from the human host. Here, we show that an <em>Mtb</em> mutant lacking the <em>mmpS4</em> and <em>mmpS5</em> genes did not grow under low iron conditions. A cytoplasmic iron reporter indicated that the double mutant experienced iron starvation even under high-iron conditions. Loss of <em>mmpS4</em> and <em>mmpS5</em> did not change uptake of carboxymycobactin by <em>Mtb</em>. Thin layer chromatography showed that the Δ<em>mmpS4/S5</em> mutant was strongly impaired in biosynthesis and secretion of siderophores. Pull-down experiments with purified proteins demonstrated that MmpS4 binds to a periplasmic loop of the associated transporter protein MmpL4. This interaction was corroborated by genetic experiments. While MmpS5 interacted only with MmpL5, MmpS4 interacted with both MmpL4 and MmpL5. These results identified MmpS4/MmpL4 and MmpS5/MmpL5 as siderophore export systems in <em>Mtb</em> and revealed that the MmpL proteins transport small molecules other than lipids. MmpS4 and MmpS5 resemble periplasmic adapter proteins of tripartite efflux pumps of Gram-negative bacteria, however, they are not only required for export but also for efficient siderophore synthesis. Membrane association of MbtG suggests a link between siderophore synthesis and transport. The structure of the soluble domain of MmpS4 (residues 52–140) was solved by NMR and indicates that mycobacterial MmpS proteins constitute a novel class of transport accessory proteins. The bacterial burden of the <em>mmpS4/S5</em> deletion mutant in mouse lungs was lower by 10,000-fold and none of the infected mice died within 180 days compared to wild-type <em>Mtb</em>. This is the strongest attenuation observed so far for <em>Mtb</em> mutants lacking genes involved in iron utilization. In conclusion, this study identified the first components of novel siderophore export systems which are essential for virulence of <em>Mtb</em>.</p> </div

MmpS4 and MmpS5 are required for siderophore secretion in <i>M. tuberculosis</i>.

<p>TLC of cell-associated and secreted siderophores extracted from cultures of wt <i>Mtb</i> H37Rv parent strain ML617, Δ<i>mmpS4</i> single deletion mutant ML472, Δ<i>mmpS5</i> single deletion mutant ML405, <i>mmpS4/S5</i> double deletion mutant ML618, Δ<i>mmpS4/S5</i> double deletion mutant fully complemented with <i>mmpS4</i> and <i>mmpS5</i> ML624, and the siderophore biosynthetic mutant Δ<i>mbtD</i>::<i>hyg</i> ML1424. Cultures were labelled with 7-[¹⁴C]-salicylic acid, which was run on the TLC as a control alongside ⁵⁵Fe-loaded cMBT and mycobactin (MBT). Lanes containing cell-associated extracts were loaded with 5,000 cpm, while media extracts were loaded with 7,500 cpm.</p

Effect of <i>mmpS4</i> and <i>mmpS5</i> on the survival of mice infected with <i>M. tuberculosis</i>.

<p>Survival of mice infected with wt <i>Mtb</i> H37Rv (ML617), Δ<i>mmpS4/S5</i> (ML618), Δ<i>mmpS4/S5</i> singly complemented with <i>mmpS5</i> (ML619), Δ<i>mmpS4/S5</i> singly complemented with <i>mmpS4</i> (ML620), or Δ<i>mmpS4/S5</i> fully complemented with <i>mmpS4</i> and <i>mmpS5</i> (ML624). Thirteen mice were infected with each strain. Mice were euthanized at day 169.</p

MmpS4 and MmpS5 are not involved in iron sensing or uptake of siderophores.

<p><b>A.</b> GFP fluorescence was measured in wt <i>Mtb</i> mc²6230, Δ<i>mmpS4/S5</i>, and Δ<i>mbtD</i>::<i>loxP</i> strains containing a <i>gfp</i>-based iron-regulated reporter construct. Strains were grown in 7H9 media and fluorescence was measured two days after the addition of carboxymycobactin (cMBT) (black bars) or blank control (grey bars). Experiments were performed in triplicate and are shown with standard deviations. <b>B.</b> Uptake of ⁵⁵Fe loaded cMBT by <i>Mtb</i> Δ<i>mmpS4/S5</i> (black circles) and Δ<i>mmpS4/S5</i> Δ<i>mbtD::hyg</i> (white triangles). Assays were performed at 37°C using a final concentration of 0.25 µM cMBT, 0.45 µCi ⁵⁵Fe in triplicate. Standard deviations are shown.</p

MmpS4 or MmpS5 is required for growth of <i>M. tuberculosis</i> under iron-limited conditions.

<p><b>A.</b> Expression of MmpS4 and MmpS5 in <i>Mtb</i>. Whole cell lysates from wt <i>Mtb</i> mc²6230, Δ<i>mmpS4</i>, Δ<i>mmpS5</i>, Δ<i>mmpS4/S5</i>, Δ<i>mmpS4/S5+mmpS4/S5</i>, Δ<i>mbtD</i>::<i>hyg</i>, and Δ<i>mmpS4/S5/mbtD</i>::<i>hyg</i> were probed by Western blot by using rabbit polyclonal antibodies raised against MmpS4 and MmpS5. The cytoplasmic fructose 1,6-bisphosphatase GlpX was used as a loading control and detected using an anti-GlpX antiserum. <b>B, C.</b> Serial dilutions of log-phase cultures of <i>Mtb</i> mc²6230, Δ<i>mmpS4</i>, Δ<i>mmpS5</i>, Δ<i>mmpS4/S5</i>, Δ<i>mmpS4/S5</i> fully complemented with <i>mmpS4</i> and <i>mmpS5</i>, Δ<i>mbtD</i>::<i>hyg</i>, and Δ<i>mmpS4/S5</i>Δ<i>mbtD</i>::<i>hyg</i> were spotted on low iron glycerol-alanine-salts (GAS) plates (<b>B</b>), or on low iron GAS plates with 5 µM hemoglobin as an iron source (<b>C</b>).</p

MmpS proteins interact with MmpL proteins.

<p><b>A.</b> Genetic interactions between MmpS4 and MmpS5 proteins and their cognate MmpL proteins. Percent of growth in iron-restricted medium (7H9 medium containing 50 µM 2,2′-dipyridyl) of triple mutants Δ<i>mmpS4/L4/S5</i> and Δ<i>mmpS4/S5/L5</i> strains and those strains complemented with <i>mmpS4</i> or <i>mmpS5</i> compared to growth in iron-rich media. <b>B.</b> Interaction of the C-terminal soluble domain of MmpS4 (residues 52–140) with the L1 loop of MmpL4 (residues 58–199) by an <i>in vitro</i> pull down assay.</p

Structure of the C-terminal soluble domain of MmpS4 (residues 52–140).

<p><b>A.</b> NMR structure of MmpS4_52–140 showing the backbone superposition of the final 20 conformers. The coordinates for the structures have been deposited in the Protein Data Bank (PDB accession code 2LW3). <b>B.</b> Cartoon depiction of a representative structure.</p

MmpS4, MmpS5 and MbtG are membrane-associated proteins.

<p>Proteins of subcellular fractions of wt <i>Mtb</i> (ML878) were extracted with 3% SDS and analyzed by SDS-polyacrylamide (10%) gel electrophoresis and Western blot using protein specific antibodies. <b>A.</b> Subcellular localization of MmpS4 and MmpS5. OmpATb, IdeR and Ag85 were used as controls for membrane, water-soluble cytoplasmic or periplasmic proteins and secreted proteins, respectively. MmpS4 and MmpS5 were detected using rabbit polyclonal antibodies. <b>B.</b> Subcellular localization of MbtG. MctB and IdeR were used as controls for membrane and water-soluble cytoplasmic or periplasmic proteins, respectively. MbtG was expressed with a C-terminal fusion of the Human influenza hemagglutinin (HA) tag which was detected using an HA-specific antibody.</p