2 research outputs found
Infrared Metrics for Fixation-Free Liver Tumor Detection
Infrared (IR) spectroscopic imaging
of human liver tissue slices
has been used to identify and characterize liver tumors. Liver tissue,
containing a liver metastasis of breast origin (mucinous carcinoma),
was surgically removed from a consenting patient and frozen without
formalin fixation or dehydration procedures, so that lipids and water
remained in the tissues. A set of IR metrics (ratios of various IR
peaks) was determined for tumors in fixation-free liver tissues. K-means
cluster analysis was used to tell tumor from nontumor. In this case,
there was a large reduction in lipid content upon going from nontumor
to tumor tissue, and a well-resolved IR spectrum of nontumor liver
lipid was obtained and analyzed. These IR metrics may someday guide
work on IR spectroscopic diagnostics on patients in the operating
room. This work also suggests utility for these methods beyond the
identification of liver tumors, perhaps in the study of liver lipids