7 research outputs found
Interaction of CSFV E2 protein with swine host factors as detected by yeast two-hybrid system.
E2 is one of the envelope glycoproteins of pestiviruses, including classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV). E2 is involved in several critical functions, including virus entry into target cells, induction of a protective immune response and virulence in swine. However, there is no information regarding any host binding partners for the E2 proteins. Here, we utilized the yeast two-hybrid system and identified fifty-seven host proteins as positive binding partners which bound E2 from both CSFV and BVDV with the exception of two proteins that were found to be positive for binding only to CSFV E2. Alanine scanning of CSFV E2 demonstrated that the binding sites for these cellular proteins on E2 are likely non-linear binding sites. The possible roles of the identified host proteins are discussed as the results presented here will be important for future studies to elucidate mechanisms of host protein-virus interactions during pestivirus infection. However, due to the limitations of the yeast two hybrid system, the proteins identified is not exhaustive and each interaction identified needs to be confirmed by independent experimental approaches in the context of virus-infected cells before any definitive conclusion can be drawn on relevance for the virus life cycle
Scheme showing CSFV E2 alanine mutants used in the present study.
<p>All indicated residues were mutated to an alanine.</p
Biological process classification of E2 interacting proteins by PANTHER analysis.
<p>Biological process classification of E2 interacting proteins by PANTHER analysis.</p
Host proteins exclusively binding CSFV E2.
<p>proteins discovered in the swine library.</p
A graphic view of the distribution of E2 positive interacting proteins, as separated into Biological processes, as defined by the PANTHER classification system.
<p>A graphic view of the distribution of E2 positive interacting proteins, as separated into Biological processes, as defined by the PANTHER classification system.</p
Proteins that interact with CSFV+BVDV E2.
<p>proteins discovered in the swine library. All other proteins discovered in the bovine library.</p