The Digital Eighteenth Century

The Digital Eighteenth Century is an ongoing collaboration between the English Department, William Hannon Library, and students at Loyola Marymount University (LMU). Its goal is to use digital tools and platforms to help students of the eighteenth century immerse themselves in the literature and culture of the period. British Literature 1660-1800: The Digital Eighteenth Century not only offeres an advanced introduction to the literature of the Enlightenment and Romanticism (1750-1830), but also introduces students to the range of projects, methods, and debates within Digital Humanities (DH). We will explore how DH might transform literary scholarship in the long eighteenth century. Will it allow scholars to discover new aspects of the literature they are studying? Can new digital tools work in tandem with qualitative analysis and close reading? Using a variety of digital tools (which will include the textual analysis program Voyant and the online database ECCO), we will explore the research and pedagogical opportunities offered by DH. While our course will explore the potential gains—scholarly, interpretive, and pedagogical—of using these digital tools, we will also discuss some theoretical and practical questions of researching, analyzing, and teaching literature using these technologies. For more information, see the course materials designed by Dermot Ryan and Melanie Hubbard. Literary movements and historical events told through timelines and maps: 18th Century Poetry Anna Barbauld Arthur Young’s Travels Through France The Crown & Anchor Society John Wesley: Methodism The Romantic Period Thomas Paine: Rights of Manhttps://digitalcommons.lmu.edu/ds-collection/1005/thumbnail.jp

Digital Humanities as Community Engagement: The Digital Watts Project

The Digital Watts Project was a graduate-level English class taught in summer of 2016 that focused on the 1965 Watts “Uprising” or “Riots.” The class worked with the Southern California Library (SCL) to make available, through a digital public humanities project, primary sources intended to expand the narrative around the events of 1965, and to situate them in a broader context of the history of race and racism in Los Angeles. Exploring the ways in which our background in the humanities could positively enrich our work with the SCL, Melanie Hubbard, a Digital Scholarship Librarian at Loyola Marymount University, and Dermot Ryan, an Associate Professor of English, designed a class that drew on literary texts, history, and information science, as well as including a number of speakers with disciplinary expertise and firsthand experience to inform the generation of metadata for this project

MIREOT: the Minimum Information to Reference an External Ontology Term

While the Web Ontology Language (OWL) provides a mechanism to import ontologies, this mechanism is not always suitable. First, given the current state of editing tools and the issues they have working with large ontologies, direct OWL imports have sometimes proven impractical for day-to-day development. Second, ontologies chosen for integration may be under active development and not aligned with the chosen design principles. Importing heterogeneous ontologies in their entirety may lead to inconsistencies or unintended inferences. In this paper we propose a set of guidelines for importing required terms from an external resource into a target ontology. We describe the guidelines, their implementation, present some examples of application, and outline future work and extensions

Both Virus and Host Components Are Important for the Manifestation of a Nef- Phenotype in HIV-1 and HIV-2

AbstractWhile it has been demonstrated that the Nef protein of simian immunodeficiency virus is obligatory for the establishment of high viral loads and the development of simian AIDS in rhesus macaques, demonstrating a critical role for the human immunodeficiency virus (HIV) Nef protein in tissue culture has been elusive. Data have been contradictory as to whether Nef has a negative or positive influence on in vitro virus replication. In an attempt to define a role for Nef during virus propagation in tissue culture and to obtain virus-host systems that could distinguish between the Nef mutant and wildtype viruses, we have introduced mutations into the nef genes of infectious molecular clones of three HIV-1 strains and two isolates of the HIV-2ROD strain and have investigated the capacity of viruses derived from them to infect a number of CD4-positive T-cell lines and peripheral blood mononuclear cells (PBMC). Mutating the nef gene of all viruses had a modest negative effect on virus production in activated PBMC. In some T-cell lines with some viruses, the effects were severe, and little or no Nef mutant virus could be detected. In other cell lines, the result of mutating the nef gene either had no effect or had a slight negative effect on the replication kinetics. Therefore, whether the consequences of loss of Nef activity can be demonstrated in vitro depends on both the particular virus and the host cell used, suggesting that Nef is exerting its activity on some cellular pathway. In addition, we describe the construction and properties of hitherto unreported infectious molecular clones of the ROD strain of HIV-2

The PAS domain-containing histidine kinase RpfS is a second sensor for the diffusible signal factor of <em>Xanthomonas campestris</em>

Summary: A cell-cell signalling system mediated by the fatty acid signal DSF controls the virulence of Xanthomonas campestris pv. campestris (Xcc) to plants. The synthesis and recognition of the DSF signal depends upon different Rpf proteins. DSF signal generation requires RpfF whereas signal perception and transduction depends upon the sensor RpfC and regulator RpfG. Detailed analyses of the regulatory roles of different Rpf proteins have suggested the occurrence of further sensors for DSF. Here we have used a mutagenesis approach coupled with high-resolution transcriptional analysis to identify XC_2579 (RpfS) as a second sensor for DSF in Xcc. RpfS is a complex sensor kinase predicted to have multiple Per/Arnt/Sim (PAS) domains, a histidine kinase domain and a C-terminal receiver (REC) domain. Isothermal calorimetry showed that DSF bound to the isolated N-terminal PAS domain with a Kd of 1.4μM. RpfS controlled expression of a sub-set of genes distinct from those controlled by RpfC to include genes involved in type IV secretion and chemotaxis. Mutation of XC_2579 was associated with a reduction in virulence of Xcc to Chinese Radish when assayed by leaf spraying but not by leaf inoculation, suggesting a role for RpfS-controlled factors in the epiphytic phase of the disease cycle.</p

Vermonters’ Opinions on Low-Dose CT Lung Cancer Screening

Introduction: Lung cancer is the number one cause of cancer death among men and women in Vermont and the United States. Smoking increases the risk of lung cancer—nearly 90% of lung cancer is due to smoking. Frequently, lung cancers do not present clinically until they are advanced stage and therefore prognosis is poor. However, if detected early lung cancers are more operable and patients have better outcomes. In December 2013 the US Preventive Services Task Force released new guidelines for lung cancer screening among current and former smokers ages 55 to 80. It is recommended that current and former (within 15 years of quitting) smokers of 30 pack years receive an annual low-dose CT scan. The objective of this project was to assess the level of knowledge and attitudes towards lung cancer screening with low-dose CT scanning among Vermonters in the Burlington area.https://scholarworks.uvm.edu/comphp_gallery/1205/thumbnail.jp

Summer habitat use and movements of invasive wild pigs (Sus scrofa) in Canadian agro-ecosystems

Resource selection informs understanding of a species’ ecology and is especially pertinent for invasive species. Since introduced to Canada, wild pigs (Sus scrofa Linnaeus, 1978) remain understudied despite recognized negative impacts on native and agricultural systems globally. Elsewhere in North America, pigs typically use forests and forage in agricultural crops. We hypothesized Canadian wild pigs would behave similarly, and using GPS locations from 15 individuals, we examined diel and seasonal resource selection and movement in the Canadian prairie region. Forests were predominately selected during the day, while corn (Zea mays L.), oilseeds, and wheat (Triticum aestivum L.) were predominately selected at night. Forests and corn were consistently selected throughout the growing season.Wetlands and forests showed greater use rates than other habitats, with evident trade-offs as crop use increased with the timing of maturation. Activity was consistent with foraging in growing crops. Results indicate diel patterns were likely a function of short-term needs to avoid daytime anthropogenic risk, while seasonal patterns demonstrate how habitats that fill multiple functional roles——food, cover, and thermoregulation——can be optimized. Understanding selection by invasive species is an important step in understanding their potential environmental impacts in novel environments and informs their management

Flexible Manufacturing Facility for Biopharmaceuticals

Monoclonal antibodies (mAbs) have the potential to treat a wide range of diseases. They possess the ability to bind target molecules in a highly specific and effective manner. Recently, great technological advances have been made to enhance the therapeutic effects of these drugs, making treatment cheaper, easier, and more effective while allowing companies to profit significantly. As a Contract Manufacturing Organization (CMO) for these products, we offer the newest technology and many flexible options for producing these proteins. Our facility is designed to produce protein products in Chinese Hamster Ovary (CHO) cells, followed by modification and purification steps. We present the option to cleave smaller antigen binding fragments (Fab) from the mAb product, removing the crystallizable fragment (Fc) which can interfere with the binding specificity of the drug. We also offer the option for polyethylene glycosylation (PEGylation), which has been shown to improve the effectiveness of these drugs. The attachment of a polyethylene glycol (PEG) molecule to the protein enhances its circulation time in the human body so that less frequent doses are needed. To demonstrate the capabilities of this flexible facility, we have modeled the production of an innovative PEGylated anti-TNF-α mAb. Celltech and Pfizer currently have similar products in Phase III clinical trials; and UCB Incorporated’s Cimzia® recently received FDA approval for the first humanized PEGylated anti-TNF-α Fab’ therapeutic protein. Many non- PEGylated TNF-α inhibitor mAbs are currently on the market to treat pathologies including rheumatoid arthritis and Crohn’s disease. PEGylated products have a clear advantage over these drugs. Our facility can produce up to 55 batches of protein product a year for a maximum yield of 993 kg. For economic analysis of this product, sales from the first year of Cimzia® were considered since this product is almost identical to the one being modeled. Producing at 75% of the total design capacity, this facility has a NPV of $1,319,592,100, an IRR of 33.51% and an ROI of 53.0%. This level of production would leave a significant amount of time remaining for other products to be manufactured as well. The additional products that the facility will produce will be mAbs of all forms (i.e. cleaved, uncleaved, PEGylated, non-PEGylated) that are protected under IP for small biotech firms that do not have the capital to build such facilities. Currently, small biotech firms are producing 81 mAbs and are looking to license production for their Phase III molecules. Clearly, this will become a very profitable CMO as we would be able to capture much of this demand. A major threat looming over the mAb market, however, is the production of small molecular inhibitors, which are currently in Phase I and Phase II clinical trials. Such molecules may be able to capture the full market since they would not only have significant delivery advantages over TNF-α inhibitors, which require injection, but also might have an enhanced side effect profile compared with biologics

Letter from the Editors

The Gettysburg Historical Journal embodies the History Department\u27s dedication to diverse learning and excellence in academics. Each year, the Journal publishes the top student work in a range of topics across the spectrum of academic disciplines with different methodological approaches to the study of history. In the words of Marc Bloch, author of The Historian\u27s Craft, history is neither watchmaking nor cabinet construction. It is an endeavor toward better understanding. In the spirit of this maxim, our authors strive to elucidate the many facets of human societies and cultures. Whether these young scholars\u27 research is focused on politics, religion, economics, environmental history, or women, gender, and sexuality studies, and the editorial staff is consistently proud of the diverse subject matter we select for publication. [excerpt