Wheel Tread Reconstruction Based on Improved Stoilov Algorithm

With the development of rail transit in terms of speed and carrying capacity, train safety problems caused by wheel tread defects and wear have become more prominent. The wheel is an important part of the train, and the wear and defects of the wheel tread are directly related to the safety of the train; therefore, wheel tread testing is a key element of train testing. In phase measuring profilometry (PMP), the virtual sine grating generated by the computer is projected onto the measured wheel tread by a digital projector, and then a camera is used to obtain the modulated deformed grating on the surface of the wheel tread. Next, the wrapped phase is obtained by the improved Stoilov algorithm, and the unwrapped phase is obtained by the phase unwrapped algorithm. Finally, the three-dimensional (3D) profile of the wheel tread is reconstructed. This paper presents an improved Stoilov algorithm based on probability and statistics. Supposing that the probability of real data was the highest, we chose the cosine square matrix value of the phase shift for processing. After ruling out the singular points of large error, we obtained the closest value to the true phase shift using the method of probability and statistics. The experimental results show that this method can effectively restrain the singular phenomenon, and the 3D profile of wheel tread can be reconstructed successfully

Application of Moire Profilometry in Three-Dimensional Profile Reconstruction of Key Parts in Railway

Moire profilometry (MP) is one of the three-dimensional (3D) topography measurement methods of structured light, which has the advantages of single frame reconstruction, high speed, no contact and high precision, and is suitable for dynamic measurement scenes. In this article, the digital MP is applied to the wheel tread measurement, the virtual grating is generated by computer to project to the object surface, the moire fringe pattern of the object is obtained by filtering, and finally the continuous phase pattern is obtained by phase unwrapping. The 3D shape reconstruction of the wheel tread is realized, and a new method of wheel tread detection is provided. At the same time, in this paper, the results of using different filters are compared, and the significance of the frequency domain filtering to MP is proved. It is necessary to choose a suitable filtering method according to different environmental conditions. At present, digital MP can be used in industrial static detection, and it can be extended to the dynamic detection of rolling wheels in the future, so as to improve the detection efficiency and realize the automatic detection of trains

Expression and Functional Analysis of the Argonaute Protein of Thermus thermophilus (TtAgo) in E. coli BL21(DE3)

The prokaryotic Argonaute proteins (pAgos) have been reported to cleave or interfere with DNA targets in a guide-dependent or independent manner. It is often difficult to characterize pAgos in vivo due to the extreme environments favored by their hosts. In the present study, we expressed functional Thermus thermophilus pAgo (TtAgo) in E. coli BL21 (DE3) cells at 37 °C. Initial attempts to express TtAgo in BL21(DE3) cells at 37 °C failed. This was not because of TtAgo mediated general toxicity to the host cells, but instead because of TtAgo-induced loss of its expression plasmid. We employed this discovery to establish a screening system for isolating loss-of-function mutants of TtAgo. The E. colifabI gene was used to help select for full-length TtAgo loss of function mutants, as overexpression of fabI renders the cell to be resistant to the triclosan. We isolated and characterized eight mutations in TtAgo that abrogated function. The ability of TtAgo to induce loss of its expression vector in vivo at 37 °C is an unreported function that is mechanistically different from its reported in vitro activity. These results shed light on the mechanisms by which TtAgo functions as a defense against foreign DNA invasion

Zwitterionic sulfhydryl Sulfobetaine stabilized platinum nanoparticles for ernhanced Dopamine detection and antitumor ability

Herein, three kinds of molecules were used to modify the surface of platinum nanoparticles (Pt NPs) to tune their surface charge. Zwitterionic thiol-functionalized sulfobetaine (SH-SB) stabilized Pt NPs (SH-SB/Pt NPs) had the highest oxidase activity and peroxidase activity in the prepared platinum nanozymes due to the generation of reactive oxygen species. In addition, a colorimetric dopamine detection method was established based on the peroxidase activity of SH-SB/Pt NPs. This method had a wide range (0-120 μM), a low detection limit (0.244 μM), and high specificity. More importantly, SH-SB/Pt NPs displayed little hemolysis and good stability in the presence of proteins. SH-SB/Pt NPs demonstrated high cytotoxicity in vitro and good antitumor ability in vivo, which was attributed to the photothermal conversion ability of SH-SB/Pt NPs and the generation of reactive oxygen species in the acidic environment. The surface modification of nanozymes using zwitterionic molecules opens a new method to improve the catalytic activity and antitumor ability of nanozymes. </p

Zwitterionic Sulfhydryl Sulfobetaine Stabilized Platinum Nanoparticles for Enhanced Dopamine Detection and Antitumor Ability

Herein, three kinds of molecules were used to modify the surface of platinum nanoparticles (Pt NPs) to tune their surface charge. Zwitterionic thiol-functionalized sulfobetaine (SH-SB) stabilized Pt NPs (SH-SB/Pt NPs) had the highest oxidase activity and peroxidase activity in the prepared platinum nanozymes due to the generation of reactive oxygen species. In addition, a colorimetric dopamine detection method was established based on the peroxidase activity of SH-SB/Pt NPs. This method had a wide range (0–120 μM), a low detection limit (0.244 μM), and high specificity. More importantly, SH-SB/Pt NPs displayed little hemolysis and good stability in the presence of proteins. SH-SB/Pt NPs demonstrated high cytotoxicity in vitro and good antitumor ability in vivo, which was attributed to the photothermal conversion ability of SH-SB/Pt NPs and the generation of reactive oxygen species in the acidic environment. The surface modification of nanozymes using zwitterionic molecules opens a new method to improve the catalytic activity and antitumor ability of nanozymes