Norepinephrine Transporter Blocker Atomoxetine Increases Salivary Alpha Amylase

It has been suggested that central norepinephrine (NE) activity may be inferred from increases in salivary alpha-amylase (SAA), but data in favor of this proposition are limited. We administered 40 mg of atomoxetine, a selective NE transporter blocker that increases central NE levels, to 24 healthy adult participants in a double-blind, placebo-controlled cross-over design. Atomoxetine administration significantly increased SAA secretion and concentrations at 75–180 min after treatment (more than doubling baseline levels). Consistent with evidence that elevation in central NE is a co-determinant of hypothalamic-pituitary-adrenal axis activity, salivary cortisol also approximately doubled at the same time points. Moreover, changes in salivary cortisol positively correlated with SAA (0.44 \u3c rho \u3c 0.56), bolstering the position that the origin of the changes in SAA reflect central NE. This work points toward the potential value of SAA as an inexpensive and non-invasive procedure to obtain information about activation of the central NE system

BAC to the future! or oligonucleotides: a perspective for micro array comparative genomic hybridization (array CGH)

The array CGH technique (Array Comparative Genome Hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. It is used in human genetics and oncology, with great promise for clinical application. Until recently primarily PCR amplified bacterial artificial chromosomes (BACs) or cDNAs have been spotted as elements on the array. The large-scale DNA isolations or PCR amplifications of the large-insert clones necessary for manufacturing the arrays are elaborate and time-consuming. Lack of a high-resolution highly sensitive (commercial) alternative has undoubtedly hindered the implementation of array CGH in research and diagnostics. Recently, synthetic oligonucleotides as arrayed elements have been introduced as an alternative substrate for array CGH, both by academic institutions as well as by commercial providers. Oligonucleotide libraries or ready-made arrays can be bought off-the-shelf saving considerable time and efforts. For RNA expression profiling, we have seen a gradual transition from in-house printed cDNA-based expression arrays to oligonucleotide arrays and we expect a similar transition for array CGH. This review compares the different platforms and will attempt to shine a light on the ‘BAC to the future’ of the array CGH technique

How soccer scouts identify talented players

Scouts of soccer clubs are often the first to identify talented players. However, there is a lack of research on how these scouts assess and predict overall soccer performance. Therefore, we conducted a large-scaled study to examine the process of talent identification among 125 soccer scouts. Through an online self-report questionnaire, scouts were asked about (1) the players’ age at which they can predict players’ soccer performance, (2) the attributes they consider relevant, and (3) the extent to which they predict performance in a structured manner. The most important results are as follows. First, scouts who observed 12-year-old and younger players perceived they could predict at older ages (13.6 years old, on average) whether a player has the potential to become a professional soccer player. This suggests that scouts are aware of the idea that early indicators of later performance are often lacking, yet do advise on selection of players at younger ages. Second, when identifying talented players, scouts considered more easily observable attributes, such as technical attributes. However, scouts described these often in a broad sense rather than in terms of specific predictors of future performance. Finally, scouts reported that they assess attributes of players in a structured manner. Yet, they ultimately based their prediction (i.e. final score) on an intuitive integration of different performance attributes, which is a suboptimal strategy according to existing literature. Taken together, these outcomes provide specific clues to improve the reliability and validity of the scouting process