Fatty acids in veterinary medicine and research

Fatty acid regulation is an essential process for all animals. A number of studies have shown that diet affects the levels/availability of fatty acids in the body but increasingly evidence shows that disease states can alter the amounts within the body too. Fatty acid levels and availability have been altered by a number of diseases, disorders and reactions including inflammatory responses, heart disease and heart failure and wound repair. They are also essential during the growth and development stages of animals. The amount of research into the consequences of different fatty acid intake and levels in various disease states and during development has increased in both humans and animals. This review presents an overview of the research undertaken to date and highlights the importance, uses and benefits of understanding the roles of fatty acids in both the healthy animal and under differing disorders and diseases

Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)

The African catfish (Clarias gariepinus) has increasingly become an economically important food source especially in Africa, Asia and Europe, with exports extending to countries throughout the world. In the last 25 years, aquaculture of this species has been particularly effective with large increases in production and this is in part due to the catfish being highly resistant to disease. In order to further understand the immune system of the catfish, the spleen was investigated using a number of staining, immunohistochemical, light and transmission electron microscope techniques in order to verify the histological features of this organ. The gross histological features of the spleen confirmed the presence of a capsule containing numerous collagen fibers surrounded the spleen of the African catfish. Melanomacrophage centers, lymphocytes and surrounding arterial vessels were the main components of the white pulp whilst the red pulp contained sinusoids, capillaries and splenic cords of erythrocytes, macrophages and lymphocytes. Transmission electron microscopy identified Langerhans-like cells which were irregular to ovoid in shape with an electron lucent cytoplasm. The cells were characterized by the presence of numerous Birbeck-like granules, varying in shape from oval, round, rod to racket shaped granules. In total, three types of Birbeck-like granules were identified. The first type had heterogenic content, the second type were cored granules and the third type were racket shaped granules. Some of the racket shaped granules were enlarged and contained granular content. The Langerhans-like cells were further identified and characterised by showing a positive reaction with antibodies to S100 and CD1a. The existence of Langerhans-like cells within the spleen helps to identify the components of the immune system within the African catfish

Is your extra X chromosome holding you back? An insight into female education and academic careers in STEMM

This review discusses whether gender inequality still exists within medical, scientific and engineering academia, with regards to the career development of academic staff. In the 1970s it was suggested that women who are talented and educated with family responsibilities tend to come across problems of self-confidence and identity when attempting to enhance their professional careers, and although many are successful in doing so, others find it more challenging. By the 1990s, it was indicated that the main gender inequality mechanism in academia is the commonly known fact that women’s career development in the academic hierarchy is slower than that of men. In the past 50 years, laws and attitudes of many societies, industries and countries, have changed to promote gender equality. What is the impact of these changes, does inequality still exist and what mechanisms exist to address these issues? This review looks in depth at the links between gender equality and continuing personal and professional development (CPPD), in which individuals at work are educated more about the workplace environment and their job roles and performance. The different types, requirements and success rates of CPPD within the scientific (especially medical) academic community is discussed with an emphasis on gender equality

Computed tomography in veterinary medicine: currently published and tomorrow’s vision

The utilisation of Computed Tomography (CT) in veterinary practice has been increasing rapidly in line with reduced cost, improved availability and the increase in expertise and technology. This review briefly examines the recent technological advancements in imaging in the veterinary sector, and explores how CT and micro-CT (μCT) have furthered basic understanding and knowledge, and influenced clinical practice and medicine. The uses of CT technology in veterinary research, especially in relation to bone, vasculature and soft tissues, are explored and compared in relation to the different species. CT is essential not only for the diagnosis and treatment of many disorders, but it is now being used to understand areas ranging from drug delivery and surgical advancements through to anatomical and educational uses throughout the world

Cell Culture Based in vitro Test Systems for Anticancer Drug Screening

The development of new high-tech systems for screening anticancer drugs is one of the main problems of preclinical screening. Poor correlation between preclinical in vitro and in vivo data with clinical trials remains a major concern. The choice of the correct tumor model at the stage of in vitro testing provides reduction in both financial and time costs during later stages due to the timely screening of ineffective agents. In view of the growing incidence of oncology, increasing the pace of the creation, development and testing of new antitumor agents, the improvement and expansion of new high-tech systems for preclinical in vitro screening is becoming very important. The pharmaceutical industry presently relies on several widely used in vitro models, including two-dimensional models, three-dimensional models, microfluidic systems, Boyden’s chamber and models created using 3D bioprinting. This review outlines and describes these tumor models including their use in research, in addition to their characteristics. This review therefore gives an insight into in vitro based testing which is of interest to researchers and clinicians from differing fields including pharmacy, preclinical studies and cell biology

Mouse Tumor Models for Advanced Cancer Immunotherapy

Recent advances in the development of new methods of cancer immunotherapy require the production of complex cancer animal models that reliably reflect the complexity of the tumor and its microenvironment. Mice are good animals to create tumor models because they are low cost, have a short reproductive cycle, exhibit high tumor growth rates, and can be easily genetically modified. However, the obvious problem of these models is the high failure rate observed in human clinical trials after promising results obtained in mouse models. In order to increase the reliability of the results obtained in mice, the tumor model should reflect the heterogeneity of the tumor, contain components of the tumor microenvironment, in particular immune cells, to which the action of immunotherapeutic drugs are directed. This review discusses the current immunocompetent and immunocompromised mouse models of human tumors that are used to evaluate the effectiveness of immunotherapeutic agents, in particular chimeric antigen receptor (CAR) T-cells and immune checkpoint inhibitors

Canine osteosarcoma in comparative oncology: Molecular mechanisms through to treatment discovery.

Cancer is a leading cause of non-communicable morbidity and mortality throughout the world, similarly, in dogs, the most frequent cause of mortality is tumors. Some types of cancer, including osteosarcoma (OSA), occur at much higher rates in dogs than people. Dogs therefore not only require treatment themselves but can also act as an effective parallel patient population for the human disease equivalent. It should be noted that although there are many similarities between canine and human OSA, there are also key differences and it is important to research and highlight these features. Despite progress using chorioallantoic membrane models, 2D and 3D in vitro models, and rodent OSA models, many more insights into the molecular and cellular mechanisms, drug development, and treatment are being discovered in a variety of canine OSA patient populations