Seed and seedling responses to inoculation with mycorrhizal fungi and root nodule bacteria: implications for restoration of degraded Mediterranean-type Tuart woodlands

Inoculation with beneficial soil microorganisms has the potential to enhance success of restoration, particularly in harsh Mediterranean-type ecosystems (MTEs). We investigated the effects of microorganisms (mycorrhizal fungi and root nodule bacteria) and planting material (seed and nursery-raised seedlings) on early establishment and growth of two key postdisturbance colonizing species with different life histories, life forms and functional types (Eucalyptus gomphocephala and Acacia saligna) under field conditions. Establishment and growth were monitored at 13months, following the first MTE drought period. For E. gomphocephala, establishment was higher for seedlings (81%) than for seeding (7.5%). Inoculation with ectomycorrhizal fungal spores was not beneficial. For A. saligna, establishment was also higher for seedlings (84%) than for seeding (42.5%). Mycorrhizal fungal inoculum had no effect on establishment or growth. This study has shown that in harsh MTE conditions, the use of seedlings is more effective than seeding in degraded woodlands even when attempting to reintroduce key colonizing species. The microorganism treatments tested did not result in significant improvement in establishment or growth

Restoring degraded woodlands

In many places on the Swan Coastal Plain Eucalyptus gomphocephala (tuart) woodlands are in decline or are degraded (Bulletin 1). This occurs for many reasons, including grazing by stock, weed invasion, logging and clearing. It is often a combination of these factors that gradually lead to a decline in the tuart populations

Look before planting: using smokewater as an inventory tool to predict the soil seed bank and inform ecological management and restoration

This study tested the efficacy of smokewater to determine the potential germination from soil seed bank in three management sites of the same National Park: a forest site prior to restoration, an ex-pine plantation site and an ex-mine site. This will provide further information to land managers so that more accurate planning can occur. Results showed that smokewater significantly increased the germination from the soil seed bank, and significant differences in the level of germination of weed species from the soil seed bank were seen between the three management sites. This use of smokewater may be a useful tool to help predict differences in the soil seed bank compared with predicting soil seed bank based on land-use history and recent condition

Tuart canopy die-off during severe drought and heatwave

A severe and sudden die-off event, occurring in the regionally significant tuart (Eucalyptus gomphocephala) woodland in Rockingham Regional Park, coincided with extreme drought and heat conditions in early 2011

Stem functional traits vary among co-occurring tree species and forest vulnerability to drought

Context: Stem functional traits are critical for tree hydraulic infrastructure and have important consequences for forest function, particularly concerning vulnerability to drought. Methods: Three stem traits, sapwood area, heartwood area, and bark area, were measured in two co-dominant forest species, Eucalyptus marginata Donn. Ex. Sm. and Corymbia calophylla (Lindl.) K.D.Hill & L.A.S.Johnson, in forest patches with low and high vulnerabilities to drought in south-western Australia. Patches of high drought vulnerability experienced die-off during a heatwave and drought in 2011, while patches of low vulnerability were largely not affected. Key results: Sapwood area was significantly higher in C. calophylla than in E. marginata, and C. calophylla maintained more sapwood per unit DBH than did E. marginata, especially in larger trees. There was a 29% smaller sapwood area in high drought-vulnerability patches than in low drought-vulnerability patches (including both species). The relationship between sapwood area and DBH varied by tree size. Small trees had a greater sapwood area in high drought-vulnerable patches, whereas larger trees had more sapwood in low drought-vulnerable patches. It is unclear whether sapwood area relationships reflect differences in leaf area or tree age. Conclusions: Observed differences in sapwood between species may help explain their differential tolerance to drought, whereas differences between drought-vulnerability sites may suggest adaptation in the studied species. Implications: Understanding the traits associated with drought vulnerability will increase our prediction of forest response to drying and warming. Strong relationships between stem traits and DBH, developed here, may help future efforts to model water-use in the Northern Jarrah Forest

Bioturbation by echidna (Tachyglossus aculeatus) in a forest habitat, south-western Australia

Bioturbation by digging animals is important for key forest ecosystem processes such as soil turnover, decomposition, nutrient cycling, water infiltration, seedling recruitment, and fungal dispersal. Despite their widespread geographic range, little is known about the role of the short-beaked echidna (Tachyglossus aculeatus) in forest ecosystems. We measured the density and size of echidna diggings in the Northern Jarrah Forest, south-western Australia, to quantify the contribution echidna make to soil turnover. We recorded an overall density of 298 echidna diggings per hectare, 21% of which were estimated to be less than 1 month old. The average size of digs was 50 ± 25 mm in depth and 160 ± 61 mm in length. After taking into account seasonal digging rates, we estimated that echidnas turn over 1.23 tonnes of soil ha−1 year−1 in this forest, representing an important role in ecosystem dynamics. Our work contributes to the growing body of evidence quantifying the role of these digging animals as critical ecosystem engineers. Given that the echidna is the only Australian digging mammal not severely impacted by population decline or range reduction, its functional contribution to health and resilience of forest ecosystems is increasingly important due to the functional loss of most Australian digging mammals

Chronic historical drought legacy exacerbates tree mortality and crown dieback during acute heatwave-compounded drought

Globally, combinations of drought and warming are driving widespread tree mortality and crown dieback. Yet thresholds triggering either tree mortality or crown dieback remain uncertain, particularly with respect to two issues: (i) the degree to which heat waves, as an acute stress, can trigger mortality, and (ii) the degree to which chronic historical drought can have legacy effects on these processes. Using forest study sites in southwestern Australia that experienced dieback associated with a short-term drought with a heatwave (heatwave-compounded drought) in 2011 and span a gradient in long-term precipitation (LTP) change, we examined the potential for chronic historical drought to amplify tree mortality or crown dieback during a heatwave-compounded drought event for the dominant overstory species Eucalyptus marginata and Corymbia calophylla. We show pronounced legacy effects associated with chronically reduced LTP (1951–1980 versus 1981–2010) at the tree level in both study species. When comparing areas experiencing 7.0% and 11.5% decline in LTP, the probability of tree mortality increased from low (0.55) in both species, and probability of crown dieback increased from high (0.74) to nearly complete (0.96) in E. marginata. Results from beta regression analysis at the stand-level confirmed tree-level results, illustrating a significant inverse relationship between LTP reduction and either tree mortality (F = 10.39, P = 0.0073) or dieback (F = 54.72, P < 0.0001). Our findings quantify chronic climate legacy effects during a well-documented tree mortality and crown dieback event that is specifically associated with an heatwave-compounded drought. Our results highlight how insights into both acute heatwave-compounded drought effects and chronic drought legacies need to be integrated into assessments of how drought and warming together trigger broad-scale tree mortality and crown dieback events

Forest die-off following global-change-type drought alters rhizosphere fungal communities

Globally, forest die-off from global-change-type drought events (hotter droughts) are of increasing concern, with effects reported from every forested continent. While implications of global-change-type drought events have been explored for above-ground vegetation, below-ground organisms have received less attention, despite their essential contributions to plant growth, survival, and ecosystem function. We investigated rhizosphere fungal communities in soils beneath trees affected by a global-change-type drought in a Mediterranean climate-type ecosystem in southwestern Australia, quantifying how fungal richness, composition and functional groups varied along a drought impact gradient. Following a forest die-off three years previously, we collected soils beneath dead and alive trees within forest exhibiting high, minimal and relatively unaffected levels of forest die-off. Rhizosphere fungal DNA was extracted from soils, amplified and subjected to high throughput sequencing. Fungal community composition varied significantly (P < 0.001) along the drought impact gradient with less richness in drought affected stands. There was some evidence of community differentiation between dead versus alive trees (P = 0.09), and no difference in rarefied richness and diversity. When considered by functional group, die-off-impacted plots had more arbuscular mycorrhizal fungi (AM) and saprotrophs, and fewer ectomycorrhizal fungi (ECM), compared with living trees from the unaffected plots. Further, within die-off plots, dead versus alive tree rhizosphere samples contained more AM, saprotrophs and pathogens, and fewer ECM. Disruptions to rhizosphere fungal communities, such as altered functional groups, can have implications for ecosystem persistence and function, particularly in regions projected to experience increased global-change-type drought events

Semi-quantitative analysis of cannabinoids in hemp (Cannabis sativa L.) using gas chromatography coupled to mass spectrometry

Background Hemp (Cannabis sativa L.) is a producer of cannabinoids. These organic compounds are of increasing interest due to their potential applications in the medicinal field. Advances in analytical methods of identifying and quantifying these molecules are needed. Method This study describes a new method of cannabinoid separation from plant material using gas chromatography-mass spectrometry (GC-MS) as the analytical tool to detect low abundance cannabinoids that will likely have implications for future therapeutical treatments. A novel approach was adopted to separate trichomes from plant material to analyse cannabinoids of low abundance not observed in raw plant extract. Required plant sample used for analysis was greatly reduced compared to other methods. Derivatisation method was simplified and deconvolution software was utilised to recognise unknown cannabinoid compounds of low abundance. Results The method produces well-separated spectra and allows the detection of major and minor cannabinoids. Ten cannabinoids that had available standards could be identified and quantified and numerous unidentified cannabinoids or pathway intermediates based on GC-MS spectra similarities could be extracted and analysed simultaneously with this method. Conclusions This is a rapid novel extraction and analytical method from plant material that can identify major and minor cannabinoids using a simple technique. The method will be of use to future researchers seeking to study the multitude of cannabinoids whose values are currently not understood

Finding just the right recipe: nutrient requirements on Christmas Island

There is no history of large-scale agriculture on Christmas Island. As a result, there is a heavy reliance on imported produce