4 research outputs found
The proangiogenic capacity of polymorphonuclear neutrophils delineated by microarray technique and by measurement of neovascularization in wounded skin of CD18-deficient mice
Growing evidence supports the concept that polymorphonuclear neutrophils (PMN) are critically involved in inflammation-mediated angiogenesis which is important for wound healing and repair. We employed an oligonucleotide microarray technique to gain further insight into the molecular mechanisms underlying the proangiogenic potential of human PMN. In addition to 18 known angiogenesis-relevant genes, we detected the expression of 10 novel genes, namely midkine, erb-B2, ets-1, transforming growth factor receptor-beta(2) and -beta(3), thrombospondin, tissue inhibitor of metalloproteinase 2, ephrin A2, ephrin B2 and restin in human PMN freshly isolated from the circulation. Gene expression was confi rmed by the RT-PCR technique. In vivo evidence for the role of PMN in neovascularization was provided by studying neovascularization in a skin model of wound healing using CD18-deficient mice which lack PMN infi ltration to sites of lesion. In CD18-deficient animals, neo- vascularization was found to be signifi cantly compromised when compared with wild- type control animals which showed profound neovascularization within the granulation tissue during the wound healing process. Thus, PMN infiltration seems to facilitate inflammation mediated angiogenesis which may be a consequence of the broad spectrum of proangiogenic factors expressed by these cells. Copyright (c) 2006 S. Karger AG, Basel
Addendum to the Corman-Drosten Review Report
After submitting our review report on Corman et al. (referred hereinafter as CD-report) and
republishing it on a scientific preprint server [50] and Researchgate.net [51] we offered the
report for public discussion at cormandrostenreview.com on 27th November 2020. The
scientific community provided additional literature, references, and analyses concerning the
CD-report and the Corman et al. manuscript. Several “advocatus diaboli” confronted us with
correct or assumed problems in our report. The most common critique of the CD-report was
the lack of “wet lab” experiments to support our concerns over the technical flaws in the
PCR protocol