Generation of Functional CLL-Specific Cord Blood CTL Using CD40-Ligated CLL APC

PMCID: PMC3526610This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

UCB-derived CLL-specific T-cells form immunosynaptic complexes with CLL target cells.

<p>(A) UCB-derived CLL-specific T-cells formed immunosynaptic complexes in 72% of conjugation events with CLL cells while naïve T-cells could only form such complexes in 11% of conjugations. Examples of synaptic complex (B) vs. non-synaptic complex (C) are shown. (D) indicates a typical analytic field in which synaptic complexes are identified by white boxes and non-synaptic complexes by red boxes. In practice, at least 20 such field were evaluated for both naïve and expanded lymphocyte populations.</p

Summary of IFN-γ ELISpot Results.

<p>Summary of IFN-γ ELISpot <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051390#s3" target="_blank">Results</a>.</p

Umbilical Cord Blood Lymphocyte Memory Phenotypes.

<p>Umbilical Cord Blood Lymphocyte Memory Phenotypes.</p

In Vivo Functional Analysis of Partitioned Leukemia-Specific T-cells.

<p>A. Administration of CLL-specific T-cells <i>in vivo</i> to CLL-engrafted mice resulted in a reduction of splenic B-cell burden from 19.3% in untreated or control-treated mice to 4.5% (p = 0.01) among mice treated with CLL-specific T-cells This was statistically indistinguishable from the 2.8% splenic B-cell content of non-CLL engrafted mice. B. Representative flow cytometry plot of splenic CD19⁺ cells. Dashed line/unfilled = isotype control. Thick black line/unfilled = CLL engrafted and treated with CLL-specific effectors. Thin black line/gray filled = CLL engrafted but untreated.</p