40 research outputs found
N-Terminal Protein Characterization by Mass Spectrometry after Cyanogen Bromide Cleavage using Combined Microscale Liquid- and Solid-Phase Derivatization
No full textA Covalent Intermediate in CD38 Is Responsible for ADP-Ribosylation and Cyclization Reactions
No full text
Rat Organic Anion Transporting Protein 1A1 (Oatp1a1): Purification and Phosphopeptide Assignment â€
No full textAmino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structure
No full textA Transition-State Analogue Reduces Protein Dynamics in Hypoxanthine-Guanine Phosphoribosyltransferase â€
No full textAmino acid sequences of mouse 2.5S nerve growth factor. I. Isolation and characterization of the soluble tryptic and chymotryptic peptides
No full textA Complex of Methylthioadenosine/<i>S</i>-Adenosylhomocysteine Nucleosidase, Transition State Analogue, and Nucleophilic Water Identified by Mass Spectrometry
No full textAn enzyme-stabilized nucleophilic water molecule has
been implicated
at the transition state of Escherichia coli methylthioadenosine nucleosidase (<i>Ec</i>MTAN) by transition
state analysis and crystallography. We analyzed the <i>Ec</i>MTAN mass in complex with a femtomolar transition state analogue
to determine whether the inhibitor and nucleophilic water could be
detected in the gas phase. <i>Ec</i>MTAN–inhibitor
and <i>Ec</i>MTAN–inhibitor–nucleophilic water
complexes were identified by high-resolution mass spectrometry under
nondenaturing conditions. The enzyme–inhibitor–water
complex is sufficiently stable to exist in the gas phase
Network of MGF modulated proteins and predicted MGF modulated pathways by Ingenuity Pathway Analysis.
No full text<p>Network of MGF modulated proteins and predicted MGF modulated pathways by Ingenuity Pathway Analysis.</p
