Immunochemical methods for the determination of mycotoxins

The state-of-the-art immunochemical methods for the determination of mycotoxins are considered. Both instrumental (enzyme-linked immunosorbent assay, polarization fluoroimmunoassay, and sensor devices) and noninstrumental methods are presented. The principles of particular methods are considered, and the examples of the use of these methods for the determination of mycotoxins from various groups in food products and animal feeds are given; the main lines of development are discussed

Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine

Gel-based and membrane-based flow-through immunoassay formats were investigated for rapid ochratoxin A (OTA) detection in red wine. The flow-through set-Up consisted of an antibody containing gel or membrane placed at the bottom of a standard solid-phase extraction column (i.e the flow-through column), combined with a clean-up column. Different clean-up methods were studied for red wine clarification and purification The optimal method consisted of passing wine, diluted with an aqueous Solution containing 1% polyethylene glycol (PEG 6000) and 5% sodium hydrogencarbonate, through strong anion exchange (SAX) silica. An immunoassay for OTA detection in red wine was optimized and a cut-off level at 2 mu g L-1 according to EU legislation was achieved with both formals A more significant colour difference between blank and spiked samples was observed for the gel-based assay making this superior to the inembiane-based assay The proposed rapid gel-based test was compared with a standard immunoaffinity column - high-performance liquid chromatography - fluorescent detection (IAC-HPLC-FLD) method and a good correlation of the results was obtained for naturally contaminated wine sample