Detection of Azuki Bean Wild Relatives Having Different Salt Tolerance Mechanism and Construction of Molecular Linkage Map of Tuber Cowpea

内容の要旨・審査結果の要旨 / 資源循環学博士(学術)三重大

A rapid quantification method for tissue Na+ and K+ concentrations in salt-tolerant and susceptible accessions in Vigna vexillata (L.) A. Rich.

A rapid quantification method for leaf sodium (Na+) and potassium (K+) concentrations was developed using a compact ion meter. Leaf ion concentrations were evaluated for species of Vigna vexillata (L.) A. Rich. after two weeks of treatment with 0–200 mM of sodium chloride. To compare the ion extraction efficiency, extraction solutions of distilled water and ammonium acetate were tested. The ion concentrations of extracts obtained by both solutions were measured using an ion meter, and the values were validated using ion chromatography. For both extraction solutions, the ion meter values were highly correlated with those of the ion chromatograph. However, correlations between ion meter and chromatograph values were largely different for Na+ and K+. The rapid quantification of ion concentrations using an ion meter developed in this study was successfully utilized for evaluating differences in leaf Na+ concentrations, K+ concentrations, and K+/Na+ ratio in species of V. vexillata

Comparison of <i>Vigna vexillata</i> linkage group with those of <i>V</i>. <i>radiata</i> and <i>V</i>. <i>unguculata</i>, and some characteristics of the constructed <i>V</i>. <i>vexillata</i> SSR linkage map.

<p>Unlinked markers: CEDG074a and VES0660</p><p>Comparison of <i>Vigna vexillata</i> linkage group with those of <i>V</i>. <i>radiata</i> and <i>V</i>. <i>unguculata</i>, and some characteristics of the constructed <i>V</i>. <i>vexillata</i> SSR linkage map.</p

Characteristics of a <i>V</i>. <i>vexillata</i> linkage map based on the SSR and RAD markers.

<p>Characteristics of a <i>V</i>. <i>vexillata</i> linkage map based on the SSR and RAD markers.</p

A genetic linkage map of <i>V</i>. <i>vexillata</i> constructed from 300 F₂ individuals of intraspecific cross between accessions V1 and V5.

<p>Map distances and marker names are shown on the left and right side of the linkage groups, respectively. Marker names in italics indicate dominant loci. Markers showing significant deviation from the expected segregation ratio at 0.05 are indicated with*. SSR markers with prefixes CED are derived from azuki bean. Markers with prefixes cp and VM are derived from cowpea. BM157 (LG6) is derived from common bean. Markers with prefixes VES are EST-SSR markers derived from azuki bean.</p

Comparative linkage maps among <i>Vigna radiata</i> (left: rad), <i>V</i>. <i>unguiculata</i> (middle: ung), and <i>V</i>. <i>vexillata</i> (right: vex) based on common SSR markers.

<p>Linkage group 4 (LG 4) of <i>V</i>. <i>vexillata</i> (vex 4) was placed to the left and LG 7 of <i>V</i>. <i>vexillata</i> (vex 7) was placed to the right of LG4 of <i>V</i>. <i>radiata</i> (rad 4) to show the relationships of common markers. The positions of common markers were connected by dotted lines. Markers which were mapped on different LGs among 3 <i>Vigna</i> species were written in red text. Major translocations were indicated by red arrows. The species abbreviation name and number in parentheses below red text marker names, e.g., (vex 5) indicate that the species and linkage group number on which corresponding marker was mapped.</p

The SSR and RAD markers linkage map of <i>V</i>. <i>vexillata</i> constructed from 286 F₂ individuals of intraspecific cross between accessions V1 and V5.

<p>Map distances and marker names are shown on the left and right side of the linkage groups, respectively. Marker names in italics indicate dominant loci. Markers showing significant deviation from the expected segregation ratio at 0.05 and at 0.01 are indicated with* and **, respectively. SSR markers with prefixes CED are derived from azuki bean (red text). Markers with prefixes cp and VM are derived from cowpea (blue text). BM157 (LG6) is derived from common bean (green text). Markers with prefixes VES are EST-SSR markers derived from azuki bean (purple text). Markers without prefixes are RAD-markers derived from <i>V</i>. <i>vexillata</i> (black text).</p