Additional file 3: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Figure S1. Expression profile of signaling-related genes. Gene expression shows a higher expression of 46 receptor kinase family genes (a), 24 RLK genes (b), 5 MAPKs genes (c), and 33 CDPKs genes (d) in Phytophthora sojae-inoculated soybean plants at 4 dpi under Si− compared to Si+ treatment. Graph shows the average relative (%) expression at each timepoint based on the highest level of expression for each gene as a measure to showcase the trend in expression dynamics. Bars represent standard error from the mean (n = 5). (TIF 371 kb

Additional file 5: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Table S4. Expression data of NB-LRRs genes. Normalized mean (n = 5), fold-change and FDR p-value of 45 receptor (NB-LRR) genes in soybean plants treated (Si+) or not (Si-) with silicon (Si) at 0, 4, 7, and 14 dpi with Phytophthora sojae. (XLSX 22 kb

Additional file 8: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Table S6. Expression data of WRKY transcription factor genes. Normalized mean (n = 5), fold-change and FDR p-value of 20 WRKY genes in soybean plants treated (Si+) or not (Si-) with silicon (Si) at 0, 4, 7, and 14 dpi with Phytophthora sojae. (XLSX 16 kb

Additional file 4: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Table S3. Expression data of signaling-related genes. Normalized mean (n = 5), fold-change and FDR p-value of 46 receptor kinase family genes (sheet 1), 24 RLK genes (sheet 2), 5 MAPKs genes (sheet 3), and 33 CDPKs genes (sheet 4) in soybean plants treated (Si+) or not (Si-) with silicon (Si) at 0, 4, 7, and 14 dpi with Phytophthora sojae. (XLSX 65 kb

Additional file 14: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Figure S5. Heat map of differentially expressed genes involved in primary metabolism. Heat map shows gene expression pattern of 580 DEGs involved in primary metabolism in soybean roots inoculated (P+) or not (P−-) with P. sojae and treated (Si+) or not (Si−-) with silicon showing a notable higher expression of genes in P. sojae-infected plants. Each gene corresponds to a colored line indicating the normalized mean (n = 5) of the differentially expressed transcripts (Fold-change ≥ 4, FDR p-value ≤ 0.01). (TIF 205 kb

Additional file 9: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Figure S3. Expression profile of a) protease inhibitors and b) polyphenol oxidase. Gene expression shows a higher expression of 13 protease inhibitor genes (a) and five polyphenol oxidase genes (b) in Phytophthora sojae-inoculated soybean plants at 4 dpi under Si− compared to Si+ treatment. Graph shows the average relative (%) expression at each timepoint based on the highest level of expression for each gene as a measure to showcase the trend in expression dynamics. Bars represent standard error from the mean (n = 5). (TIF 163 kb

Additional file 2: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Table S2. Normalized expression of differentially expressed genes in soybean roots infected with Phytophthora sojae. In total, 3294 genes were differentially expressed as a result of P. sojae infection at day 4 after inoculation (P+Si−). The normalized mean (n = 5) of the differentially expressed transcripts (Fold-change ≥4, FDR p-value ≤0.01) are presented in soybean roots inoculated (P+) or not (P−) with P. sojae and treated (Si+) or not (Si−) with silicon. (XLSB 215 kb

Additional file 12: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Table S8. Expression data of secondary metabolism-related genes. Normalized mean (n = 5), fold-change and FDR p-value of 31 genes involved in flavonoid metabolism (sheet 1), nine genes involved in isoflavone metabolism (sheet 2) and 15 genes involved in isoprenoid metabolism (sheet 3) in soybean plants treated (Si+) or not (Si-) with silicon (Si) at 0, 4, 7, and 14 dpi with Phytophthora sojae. (XLSX 48 kb

Additional file 11: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Figure S4. Expression profile of secondary metabolism-related genes. Gene expression shows a higher expression of 31 genes involved in flavonoid metabolism (a), nine genes involved in isoflavone metabolism (b) and 15 genes involved in isoprenoid metabolism (c) in Phytophthora sojae-inoculated soybean plants at 4 dpi under Si−- compared to Si+ treatment. Graph shows the average relative (%) expression at each timepoint based on the highest level of expression for each gene as a measure to showcase the trend in expression dynamics. Bars represent standard error from the mean (n = 5). (TIF 261 kb

Additional file 6: of Silicon protects soybean plants against Phytophthora sojae by interfering with effector-receptor expression

Table S5. Expression data of PR genes. Normalized mean (n = 5), fold-change and FDR p-value of 11 pathogenesis-related (PR) genes in soybean plants treated (Si+) or not (Si-) with silicon (Si) at 0, 4, 7, and 14 dpi with Phytophthora sojae. (XLSX 12 kb