Experimental paradigm.

<p>Facial emotion and object discrimination tasks were presented in alternating individual blocks for . Between task conditions, a white fixation cross was presented for to serve as a baseline condition. Each task block was repeated five times, yielding a total paradigm length of . The faces, displayed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050050#pone-0050050-g001" target="_blank">Figure 1</a>, were obtained from the NimStim facial stimulus set <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050050#pone.0050050-Tottenham1" target="_blank">[111]</a>. We informed Nim Tottenham about our submission and obtained written consent to use it under the license model of PLoS ONE.</p

Habituation effects within amygdalae, orbitofrontal cortex and thalamus.

<p>When comparing mean BOLD responses of individual emotion discrimination task blocks, patient group (red bars) showed significant adaptations towards emotional stimuli, which are not observed in the control group (blue bars).</p

Psychometric assessment of participants.

<p>Hamilton Anxiety Rating Scale (HAM-A), Spielberger State-Trait Anxiety Inventory for Adults (STAI), and Liebowitz Social Anxiety Scale (LSAS) were used to evaluate psychiatric status and quantify severity of anxiety symptoms. Table shows mean scores standard deviation of SAD patient and healthy control group.</p

Task-related effects.

<p>Second-level ANOVA revealed eight regions significantly more active ( FWE corrected, voxel minimum cluster size) in the emotional face discrimination task compared to the object discrimination task. These task-relevant areas were used as regions of interest for the subsequent assessment of group-related effects. Atlas information and corresponding Brodmann areas (BA) taken from Talairach-Tournoux Atlas. <i>T</i>-values for degrees of freedom.</p

SPMs of habituation effects within the limbic system and orbitofrontal cortex.

<p>Peak voxels of clusters showing task-related differences (, for illustration purpose, cf. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050050#pone-0050050-g002" target="_blank">Figure 2</a>) were used as center voxels for ROIs (), which are shown in the map of block 5. The presented slice corresponds to slice in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050050#pone-0050050-g002" target="_blank">Figure 2</a>.</p

[¹⁸F]FEPPA: Improved Automated Radiosynthesis, Binding Affinity, and Preliminary in Vitro Evaluation in Colorectal Cancer

The overexpression of the translocator protein (TSPO) has been amply reported for a variety of conditions, including neurodegenerative disorders, heart failure, and cancer. Thus, TSPO has been proposed as an excellent imaging biomarker, allowing, in this manner, to obtain an accurate diagnosis and to follow disease progression and therapy response. Accordingly, several radioligands have been developed to accomplish this purpose. In this work, we selected [¹⁸F]­FEPPA, as one of the clinical established tracers, and assessed its in vitro performance in colorectal cancer. Moreover, we setup an improved radiosynthesis method and assessed the in vitro binding affinity of the nonradioactive ligand toward the human TSPO. Our results show an excellent to moderate affinity, in the subnanomolar and nanomolar range, as well as the suitability of [¹⁸F]­FEPPA as an imaging agent for the TSPO in colorectal cancer

Bar chart plotting serotonin transporter binding potential (5-HTT BP_ND) according to BNDF Val66Met genotype status.

<p>Values at the y-axis represent 5-HTT BP_ND in pooled healthy subjects and depressive patients. Binding potential is separated for val/val and met-carriers, respectively, x-axis shows regions of interest. Because healthy subjects and depressive patients were pooled here, regions do, but values do not correspond to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106810#pone-0106810-t003" target="_blank">table 3</a>. ACC: anterior cingulate cortex, AMY: amygdala, MCC: medial cingulate cortex, HIPP: hippocampus, CAUD: caudatum, PUT: putamen, THAL: thalamus, STRIA: striatum, MID: Midbrain, NACC: nucleus accumbens.</p

Bar chart plotting serotonin-1A binding potential (5-HT_1A BP_ND) according to BNDF Val66Met genotype status.

<p>Values at the y-axis represent 5-HT_1A BP_ND separated for val/val and met-carrier, respectively, x-axis shows regions of interest. Regions and values correspond to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106810#pone-0106810-t002" target="_blank">table 2</a>. ACC: anterior cingulate cortex, AMY: amygdala, MCC: medial cingulate cortex, HIPP: hippocampus, INS: insula, paraHIPP: parahippocampus, PCC: posterior cingulate cortex, TempPole: temporal pole, DRN: dorsal raphe nucleus.</p

Post-hoc t-tests comparing serotonin-1A receptor (5-HT_1A) binding potential (BP_ND) according to BDNF Val66Met genotype status in 51 healthy subjects.

<p>Post-hoc t-tests comparing serotonin-1A receptor (5-HT_1A) binding potential (BP_ND) according to BDNF Val66Met genotype status in 51 healthy subjects.</p

Post-hoc t-tests comparing serotonin transporter (5-HTT) binding potential (BP_ND) according to BDNF Val66Met genotype status in 25 healthy subjects and 16 depressed patients.

<p>Post-hoc t-tests comparing serotonin transporter (5-HTT) binding potential (BP_ND) according to BDNF Val66Met genotype status in 25 healthy subjects and 16 depressed patients.</p