Die SAGAT-Methode: Welche Informationen muss der Towerlotse erfassen?

<div><p>DNA methylation regulates gene expression in many organisms. In eukaryotes, DNA methylation is associated with gene repression, while it exerts both activating and repressive effects in the Proteobacteria through largely locus-specific mechanisms. Here, we identify a critical DNA methyltransferase in <i>M. tuberculosis</i>, which we term MamA. MamA creates N⁶-methyladenine in a six base pair recognition sequence present in approximately 2,000 copies on each strand of the genome. Loss of MamA reduces the expression of a number of genes. Each has a MamA site located at a conserved position relative to the sigma factor −10 binding site and transcriptional start site, suggesting that MamA modulates their expression through a shared, not locus-specific, mechanism. While strains lacking MamA grow normally <i>in vitro</i>, they are attenuated in hypoxic conditions, suggesting that methylation promotes survival in discrete host microenvironments. Interestingly, we demonstrate strikingly different patterns of DNA methyltransferase activity in different lineages of <i>M. tuberculosis</i>, which have been associated with preferences for distinct host environments and different disease courses in humans. Thus, MamA is the major functional adenine methyltransferase in <i>M. tuberculosis</i> strains of the Euro-American lineage while strains of the Beijing lineage harbor a point mutation that largely inactivates MamA but possess a second functional DNA methyltransferase. Our results indicate that MamA influences gene expression in <i>M. tuberculosis</i> and plays an important but strain-specific role in fitness during hypoxia.</p></div

Transcriptional start site (TSS)-mapping reveals a consistent spatial relationship between MamA sites and TSSs of methylation-affected genes.

<p>TSSs in strain H37Rv were mapped by the strategy outlined in (A). mRNA was circularized before random-primed synthesis of cDNA. Dashes indicate the variable 3′ end of an mRNA. Gene-specific primers were then used to amplify and sequence 5′-3′ junctions. Junctions appear as transitions from clean to messy sequence due to the variable 3′ ends. (B) TSS-mapping sequence traces are shown for the four genes whose expression is reproducibly affected by MamA. MamA sites, putative sigma factor −10 binding sites, TSSs and ORFs are shown as indicated in the key.</p

MamA is a DNA methyltransferase that protects <i>lppC</i> from endonucleolytic cleavage.

<p>(A) Southern blotting strategy to assess the status of a PvuII site near the 3′ end of <i>lppC</i>. Genomic DNA was digested with PvuII and analyzed by Southern blot with a probe hybridizing as shown. Fully cleaved DNA generates a 1.8 kb product, while protected DNA produces a 2.1 kb product. (B) DNA from the vaccine strain <i>M. bovis</i> BCG and from <i>M. tuberculosis</i> strains of the Euro-American lineage (Erdmann and CDC1551) is partially protected from PvuII cleavage while DNA from a Beijing lineage strain (HN878) is not. (C) Genetic deletion of <i>mamA</i> abrogates protection of <i>lppC</i>, while deletion of <i>hsdM</i> does not affect protection. (D) Protection is restored by complementation of a <i>ΔmamA</i> strain with an ectopic copy of <i>mamA</i>, but not by empty vector or <i>mamA^E270A</i>. (E) Sequence context of the assayed PvuII site. Underlined bases are predicted to block PvuII if methylated.</p

Several genes have lower expression levels in a <i>ΔmamA</i> strain.

<p>Expression of each gene was determined by quantitative PCR in the indicated H37Rv-derived strains and is displayed as a relative value compared to expression of the housekeeping gene <i>sigA</i> in the same strain. Values shown are the mean of three technical replicates. Error bars denote standard deviation. (*) denotes P<0.05 compared to the wildtype and complemented strains (ANOVA with Tukey's post test). The wildtype and complemented strains were not significantly different from each other for any of the genes tested. The experiment was performed using RNA from different cultures than those used to prepare RNA for microarrays.</p

Quantitation of total N⁶-MdA content in <i>M. tuberculosis</i>.

<p>Genomic DNA from the indicated strains was digested to individual nucleosides and methylation content determined by liquid chromatography-coupled tandem mass spectrometry. Results are expressed as the amount of N⁶-MdA per nucleotide (left axis) and percentage of adenines that are methylated in each genome (right axis). Each represents at least three biological replicates. Outliers were removed using Grubbs criteria and error bars represent ± standard deviation. (A) Analysis of the contribution of wildtype and mutant forms of MamA to total adenine methylation levels in strain H37Rv. (B) Analysis of the contributions of MamA and HsdM to total adenine methylation levels in Euro-American (H37Rv) and Beijing (HN878) strain backgrounds.</p

Sequence trace comparison identifies the target base and minimal recognition sequence of MamA.

<p>Plasmids containing putative MamA-recognition motifs were propagated in the indicated bacterial strains, isolated and sequenced. Sequence traces shown are representative of at least 2–3 biological replicates. (A) The 10 base pair sequence shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat-1003419-g001" target="_blank">Figure 1E</a> supports methylation of one adenine on each strand in wildtype H37Rv, as evidenced by increased thymine peak areas relative to the identical sequence context in <i>E. coli</i> and methylation-deficient strains of <i>M. tuberculosis</i>. See <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat.1003419.s002" target="_blank">Figure S2</a> for quantification of peak areas. (B) Schematic depiction of the positions of N⁶-methyladenine residues. (C) A six base pair core sequence is sufficient to direct MamA-mediated methylation (bold in panel B). See <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat.1003419.s008" target="_blank">Table S1</a> for a complete list of tested sequences. (D) Positions of MamA recognition sequences are shown schematically on the 4.4 Mb <i>M. tuberculosis</i> genome.</p

Deletion of <i>mamA</i> does not grossly affect growth rate or fitness of <i>M. tuberculosis</i> during mouse infection.

<p>(A) The indicated H37Rv-derived strains were normalized at a calculated optical density of 0.01 in Sauton's media and monitored by optical density on the days indicated. Points indicate the mean of triplicate cultures and error bars denote standard deviation. Similar results were obtained in 7H9 medium and by plating for CFU. (B) Mice were infected by the aerosol route with approximately 10,000 CFU of a mixture of unmarked wildtype H37Rv and one of three isogenic <i>mamA</i> mutants marked with kanamycin resistance. Groups of four mice per condition were sacrificed at the indicated time points and the lung burden of total and marked bacilli was determined. The mean proportion of marked bacteria is indicated. Error bars denote standard deviation.</p

Genes with expression differences of 1.5-fold or greater in Δ<i>mamA</i> or complemented strains compared to the wildtype parent (H37Rv) in aerobic growth conditions.

a<p>ANOVA.</p>b<p>Method of Benjamini and Hochberg.</p>c<p>Distance from start of ORF to nearest upstream MamA site.</p>d<p>Distance from start of first ORF in operon (Rv3083) to nearest upstream MamA site.</p>e<p>Distances in wildtype/Δ<i>mamA</i> and complemented strains, respectively; complementation vector integrates in this region.</p>f<p>Complementation vector integrates in this gene.</p