30 research outputs found
5‑Diethylamino-naphthalene-1-sulfonyl Chloride (DensCl): A Novel Triplex Isotope Labeling Reagent for Quantitative Metabolome Analysis by Liquid Chromatography Mass Spectrometry
We
describe a new set of isotope reagents, <sup>12</sup>C<sub>4</sub>-, <sup>12</sup>C<sub>2</sub><sup>13</sup>C<sub>2</sub>-, and <sup>13</sup>C<sub>4</sub>-5-diethylamino-naphthalene-1-sulfonyl chloride
(DensCl), in combination with liquid chromatography Fourier-transform
ion cyclotron resonance mass spectrometry (LC-FTICR-MS), for improved
analysis of the amine- and phenol-containing submetabolome. The synthesis
of the reagents is reported, and an optimized derivatization protocol
for labeling amines and phenols is described. To demonstrate the utility
of the triplex reagents for metabolome profiling of biological samples,
urine samples collected daily from a healthy volunteer over a period
of 14 days were analyzed. The overall workflow is straightforward,
including differential isotope labeling of individual samples and
a pooled sample that serves a global internal standard, mixing of
the isotope differentially labeled samples and LC-MS analysis for
relative metabolome quantification. Comparing to the dansyl chloride
(DnsCl) duplex isotope reagents, the new triplex DensCl reagents offer
the advantages of improved metabolite detectability due to enhanced
sensitivity (i.e., about 1000 peak pairs detected by DensCl labeling
vs about 600 peak pairs detected by DnsCl labeling) and analysis speed
(i.e., simultaneous analysis of two comparative samples by DensCl
vs only one comparative sample analyzed by DnsCl)
Adipic acid&#8211;2,6-bis(1H-benzimidazol-2-yl)pyridine&#8211;water (1/2/4)
The asymmetric unit of the title hydrated co-crystal, 2C19H13N5&#183;C6H10O4&#183;4H2O, consists of one 2,6-bis(1H-benzimidazol-2-yl)pyridine molecule, half of an adipic acid molecule (bisected by an inversion center) and two water solvates. In the crystal, N&#8212;H...O, O&#8212;H...O and O&#8212;H...N hydrogen bonds and &#960;&#8211;&#960; interactions [centroid&#8211;centroid distances = 3.769&#8197;(2) and 3.731&#8197;(2)&#8197;&#197;] form a three-dimensional supramolecular structure
5‑Diethylamino-naphthalene-1-sulfonyl Chloride (DensCl): A Novel Triplex Isotope Labeling Reagent for Quantitative Metabolome Analysis by Liquid Chromatography Mass Spectrometry
We
describe a new set of isotope reagents, <sup>12</sup>C<sub>4</sub>-, <sup>12</sup>C<sub>2</sub><sup>13</sup>C<sub>2</sub>-, and <sup>13</sup>C<sub>4</sub>-5-diethylamino-naphthalene-1-sulfonyl chloride
(DensCl), in combination with liquid chromatography Fourier-transform
ion cyclotron resonance mass spectrometry (LC-FTICR-MS), for improved
analysis of the amine- and phenol-containing submetabolome. The synthesis
of the reagents is reported, and an optimized derivatization protocol
for labeling amines and phenols is described. To demonstrate the utility
of the triplex reagents for metabolome profiling of biological samples,
urine samples collected daily from a healthy volunteer over a period
of 14 days were analyzed. The overall workflow is straightforward,
including differential isotope labeling of individual samples and
a pooled sample that serves a global internal standard, mixing of
the isotope differentially labeled samples and LC-MS analysis for
relative metabolome quantification. Comparing to the dansyl chloride
(DnsCl) duplex isotope reagents, the new triplex DensCl reagents offer
the advantages of improved metabolite detectability due to enhanced
sensitivity (i.e., about 1000 peak pairs detected by DensCl labeling
vs about 600 peak pairs detected by DnsCl labeling) and analysis speed
(i.e., simultaneous analysis of two comparative samples by DensCl
vs only one comparative sample analyzed by DnsCl)
5‑Diethylamino-naphthalene-1-sulfonyl Chloride (DensCl): A Novel Triplex Isotope Labeling Reagent for Quantitative Metabolome Analysis by Liquid Chromatography Mass Spectrometry
We
describe a new set of isotope reagents, <sup>12</sup>C<sub>4</sub>-, <sup>12</sup>C<sub>2</sub><sup>13</sup>C<sub>2</sub>-, and <sup>13</sup>C<sub>4</sub>-5-diethylamino-naphthalene-1-sulfonyl chloride
(DensCl), in combination with liquid chromatography Fourier-transform
ion cyclotron resonance mass spectrometry (LC-FTICR-MS), for improved
analysis of the amine- and phenol-containing submetabolome. The synthesis
of the reagents is reported, and an optimized derivatization protocol
for labeling amines and phenols is described. To demonstrate the utility
of the triplex reagents for metabolome profiling of biological samples,
urine samples collected daily from a healthy volunteer over a period
of 14 days were analyzed. The overall workflow is straightforward,
including differential isotope labeling of individual samples and
a pooled sample that serves a global internal standard, mixing of
the isotope differentially labeled samples and LC-MS analysis for
relative metabolome quantification. Comparing to the dansyl chloride
(DnsCl) duplex isotope reagents, the new triplex DensCl reagents offer
the advantages of improved metabolite detectability due to enhanced
sensitivity (i.e., about 1000 peak pairs detected by DensCl labeling
vs about 600 peak pairs detected by DnsCl labeling) and analysis speed
(i.e., simultaneous analysis of two comparative samples by DensCl
vs only one comparative sample analyzed by DnsCl)
Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice
This study mainly investigated the influences of Lactobacillus plantarum CQPC05 (CQPC05) isolated from pickled vegetables on mouse constipation caused by activated carbon water. We used 16S rDNA technology to identify the microorganism, and activated carbon to establish a mouse constipation model. After the mice received L. plantarum (109 CFU/mL) by gavage, small intestine tissue sections were collected. The serum indices and small intestine-related mRNA expression were obtained. A strain of L. plantarum was identified, and named CQPC05. The body weight and activated carbon progradation rate in the mice of the CQPC05 treatment groups were significantly higher than those in the control group, and the excretion time of the first tarry stool was earlier than that of the control group. The results of serum indices indicated that serum gastrin (Gas), endothelin (ET), and acetylcholinesterase (AchE) levels in the CQPC05 treatment groups were significantly higher than those in the control group, while the somatostatin (SS) level was significantly lower. Compared to the constipation control group, the result of q-PCR demonstrated that CQPC05 could up-regulate the mRNA expression of c-Kit (stem cell factor receptor), SCF (stem cell factor), and GDNF (glial cell-derived neurotrophic factor) genes, and down-regulate the expression of TRPV1 (transient receptor potential cation channel subfamily V member 1) and iNOS (inducible nitric oxide synthase). In conclusion, L. plantarum CQPC05 can effectively alleviate constipation, and has good probiotic potential and application value
Preventive Effect of Alkaloids from Lotus plumule on Acute Liver Injury in Mice
Lotus plumule is a traditional Chinese food that can be used as a beverage. In this study, three kinds of Lotus plumules from different regions of China were selected to observe the preventive effects of extracted alkaloids on CCl4-induced liver injuries. Animal experiments revealed that alkaloids extracted from Lotus plumules decreased the serum AST (aspartate aminotransferase), ALT (alanine aminotransferase), and TBIL (total bilirubin) levels, enhanced SOD (superoxide dismutase) activity, and reduced MDA (malondialdehyde) level in the liver tissues of mice with liver injury. H&E observation confirmed that alkaloids from Lotus plumules could alleviate CCl4-induced injuries of liver tissues and inhibit the inflammatory effect on hepatocytes. Further qPCR experiments also demonstrated that alkaloids from Lotus plumules upregulated the expression of IκB-α (inhibitor of NF-κB alpha), Cu/Zn-SOD (copper/zinc superoxide dismutase), Mn-SOD (manganese superoxide dismutase), and CAT (catalase) mRNA and downregulated TNF-α (tumor necrosis factor alpha) and NF-κB (nuclear factor kappa B) expression in the liver tissues of mice with liver injury. All three kinds of alkaloids from Lotus plumules could prevent CCl4-induced liver injuries by regulating the levels of oxidative stress and inflammation in mice, and the therapeutic effect was comparable to that of silymarin, the medicine commonly used in the treatment of liver diseases. In summary, alkaloids from Lotus plumules contain bioactive substances with hepatic protective efficacy and possess potential application value in the field of functional food
Anti-Obesity Effects of Lactobacillus fermentum CQPC05 Isolated from Sichuan Pickle in High-Fat Diet-Induced Obese Mice through PPAR-α Signaling Pathway
Sichuan pickle is a traditional fermented food in China which is produced by the spontaneous fermentation of Chinese cabbage. In this study, the anti-obesity effects of a new lactic acid bacterium (Lactobacillus fermentum CQPC05, LF-CQPC05) isolated from Sichuan pickles were assessed in vivo. An obese animal model was established in mice by inducing obesity with high-fat diet. Both serum and tissues were collected from the mice, and then subjected to qPCR and Western blot analyses. The results showed that LF-CQPC05 could decrease the values of hepatosomatic, epididymal fat, and perirenal fat indices that were induced by a high-fat diet in mice. Moreover, LF-CQPC05 reduced the levels of alanine aminotransferase (ALT), aspartate aminotransaminase (AST), total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C), and increased the level of high-density lipoprotein cholesterol (HDL-C) in both serum samples and liver tissues of obese mice fed with a high-fat diet. Pathological observations demonstrated that LF-CQPC05 could alleviate the obesity-induced pathological changes in the liver tissue of mice, and reduce the degree of adipocyte enlargement. The results of qPCR and Western blot analyses further indicated that LF-CQPC05 upregulated the mRNA and protein expression levels of lipoprotein lipase (LPL), PPAR-α: peroxisome proliferator-activated receptor-alpha (PPAR-α), (cholesterol 7 alpha-hydroxylase) CYP7A1, and carnitine palmitoyltransferase 1 (CPT1A), and downregulated the expression levels of peroxisome proliferator-activated receptor-gamma (PPAR-γ) and CCAAT enhancer-binding protein alpha (C/EBP-α) in both liver tissue and epididymal adipose tissue. Taken altogether, this study reveals that LF-CQPC05 can effectively inhibit high-fat diet-induced obesity. Its anti-obesity effect is comparable to that of l-carnitine, and is superior to that of Lactobacillus delbrueckii subsp. bulgaricus, a common strain used in the dairy industry. Therefore, LF-CQPC05 is a high-quality microbial strain with probiotic potential
Preventive Effect of Lactobacillus fermentum CQPC08 on 4-Nitroquineline-1-Oxide Induced Tongue Cancer in C57BL/6 Mice
Lactobacillus fermentum CQPC08 (LF-CQPC08) is a newly discovered strain of bacteria isolated and identified from traditional pickled vegetables in Sichuan, China. We used 4-nitroquinoline 1-oxide to establish an experimental tongue cancer mouse model to evaluate the preventive effect of LF-CQPC08 on tongue cancer in vivo. Lactobacillus delbruechii subsp. bulgaricus, is a common commercial strain and is used as a positive control to compare the effect with LF-CQPC08. The preventive strength and mechanism of LF-CQPC08 on tongue cancer were determined by measuring the biochemical indicators in mouse serum and tissues. Our results showed LF-CQPC08 inhibits the decline of splenic index, thymus index, percentage of phagocytic macrophages, and phagocytic index effectively. LF-CQPC08 also increased levels of mouse serum granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), immunoglobulin (Ig)G, IgM levels of serum interleukin (IL)-4, IL-12, tumor necrosis factor-alpha, and interferon-gamma levels, thereby inhibiting the decline in immunity caused by tongue cancer. It also increased the activity levels of superoxide dismutase and glutathione peroxidase and decreased the levels of malondialdehyde in the tissues of the tongue cancer mouse model, thereby suppressing the oxidative stress damage in the tissue caused by tongue cancer. Through quantitative PCR, LF-CQPC08 upregulated the mRNA expression of nuclear factor-erythroid 2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1), glutathione-S-transferases-π (GST-π), and Bcl-2-associated X protein (Bax), and downregulated the mRNA expression of p53, p63, p73, phosphatase and tensin homolog (PTEN), B-cell lymphoma 2 (Bcl-2) and B-cell lymphoma-extra large (Bcl-xL) in the tongue tissues of the tongue cancer mouse. These results indicated that LF-CQPC08 reduced the influence of tongue cancer on the immune system and oxidative balance and improved the immunity and enhanced antioxidant capacity of the mouse model, thereby preventing tongue cancer. LF-CQPC08 could be used as a microbial resource with a preventive effect on tongue cancer
Lactobacillus plantarum KSFY06 on d-galactose-induced oxidation and aging in Kunming mice
Yogurt from Xinjiang, China, is a traditional Chinese fermented food rich in beneficial microorganisms, such as Lactobacillus plantarum KSFY06. In this study, the effect of KSFY06 on oxidative aging was investigated using live animal experiments. Molecular biological methods were used to analyze the serum and tissues of mice with oxidative aging induced by d-galactose, which showed that KSFY06 can inhibit the decline of heart, liver, spleen, and kidney caused by aging. The KSFY06 strain increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and glutathione (GSH) in serum and liver of aging mice, while the content of malondialdehyde (MDA) is reduced. Pathological observation showed that KSFY06 alleviated damage to the liver, spleen, and skin of oxidative aging mice. qPCR showed that, at high dose (2 × 109 cfu/kg per day), KSFY06 upregulates copper/zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), catalase (CAT) mRNA expression, and its downstream inducible nitric oxide synthase (iNOS) mRNA expression in liver and spleen tissues induced by d-gal. To a certain extent, these findings indicate that L. plantarum KSFY06 is able to protect against oxidative stress in the d-gal-induced aging model. In conclusion, L. plantarum KSFY06 may provide a potential research value in the prevention or alleviation of related diseases caused by oxidative stress
Immunomodulatory Effect of Tremella Polysaccharides against Cyclophosphamide-Induced Immunosuppression in Mice
Polysaccharides are closely associated with immune regulation, but there are different polysaccharide effects from different sources. In this study, the aim was to investigate the effect of tremella polysaccharides (TP) in cyclophosphamide-induced immunodeficient mice. We observed the thymus and spleen index, liver and spleen pathological changes, and the levels of IL-2, IL-12, INF-γ, TGF-β and Ig G in serum, and we also noted the mRNA expression of IL-1β, IL-4, IL-12 and TGF-β in liver and spleen. Besides, we also measured the best effects of different doses of TP (Low-TP was 20 mg/kg·BW, Middle-TP was 40 mg/kg·BW, and High-TP was 80 mg/kg·BW) on cyclophosphamide-induced immunosuppressed mice. The results were remarkable, and suggested that TP had a significant effect for enhancing immunity in cyclophosphamide-induced immunosuppression, and the immune enhancement of High-TP had the best results in TP-treated mice. It could significantly increase the thymus and spleen index, alleviate pathological features of immunosuppression such as the arrangement of liver sinusoid and hepatic plates was disordered, massive inflammatory cells infiltrated and fatty degeneration of hepatocytes in liver, and red pulp and white pulp were intermixed, splenic corpuscles demolished and disappeared, splenic sinusoid extended, and lymphocytes of spleen were reduced in spleen. Besides, it could also up-regulate serum levels of IL-2, IL-12, INF-γ and Ig G, reduce the level of TGF-β in serum, markedly promote mRNA expression of IL-1β, IL-4 and IL-12 in liver and spleen, and suppress mRNA expression of TGF-β. Above all, TP showed preventive effect for cyclophosphamide-induced immunosuppressed mice