An Optimized and Efficient CRISPR/Cas9 System for the Endophytic Fungus Pestalotiopsis fici

Endophytic fungi are emerging as attractive producers of natural products with diverse bioactivities and novel structures. However, difficulties in the genetic manipulation of endophytic fungi limit the search of novel secondary metabolites. In this study, we improved the polyethylene glycol (PEG)-mediated protoplast transformation method by introducing the CRISPR/Cas9 system into endophytic fungus Pestalotiopsis fici. Using this approach, we performed genome editing such as site-specific gene insertion, dual-locus mutations, and long DNA fragment deletions in P. fici efficiently. The average efficiency for site-specific gene insertion and two-site gene editing was up to 48.0% and 44.4%, respectively. In addition, the genetic manipulation time with long DNA fragment (5–10 kb) deletion was greatly shortened to one week in comparison with traditional methods such as Agrobacterium tumefaciens-mediated transformation (ATMT). Taken together, the development of the CRISPR/Cas9 system in the endophytic fungus will accelerate the discovery of novel natural products and further biological study

Conversion Characteristics and Production Evaluation of Styrene/o-Xylene Mixtures Removed by DBD Pretreatment

The combination of chemical oxidation methods with biotechnology to removal recalcitrant VOCs is a promising technology. In this paper, the aim was to identify the role of key process parameters and biodegradability of the degradation products using a dielectric barrier discharge (DBD) reactor, which provided the fundamental data to evaluate the possibilities of the combined system. Effects of various technologic parameters like initial concentration of mixtures, residence time and relative humidity on the decomposition and the degradation products were examined and discussed. It was found that the removal efficiency of mixed VOCs decreased with increasing initial concentration. The removal efficiency reached the maximum value as relative humidity was approximately 40%–60%. Increasing the residence time resulted in increasing the removal efficiency and the order of destruction efficiency of VOCs followed the order styrene > o-xylene. Compared with the single compounds, the removal efficiency of styrene and o-xylene in the mixtures of VOCs decreased significantly and o-xylene decreased more rapidly. The degradation products were analyzed by gas chromatography and gas chromatography-mass spectrometry, and the main compounds detected were O3, COx and benzene ring derivatives. The biodegradability of mixed VOCs was improved and the products had positive effect on biomass during plasma application, and furthermore typical results indicated that the biodegradability and biotoxicity of gaseous pollutant were quite depending on the specific input energy (SIE)

Use of a Night Float System to Comply With Resident Duty Hours Restrictions:Perceptions of Workplace Changes and Their Effects on Professionalism

Purpose Although some evidence suggests that resident duty hours reforms can lead to shift-worker mentality and loss of patient ownership, other evidence links long hours and fatigue to poor work performance and loss of empathy, suggesting the restrictions could positively affect professionalism. The authors explored perceived impacts of a 16-hour duty restriction, achieved using a night float (NF) system, on the workplace and professionalism. Method In 2013, the authors conducted semistructured interviews with 18 residents, 9 staff physicians, and 3 residency program directors in the McGill University core internal medicine residency program regarding their perceptions of the program's 12-hour shift-based NF system. Interviews were transcribed and coded for common themes. The authors used a descriptive qualitative methodology. Results Participants viewed implementation of the NF system as leading to decreased physical and mental exhaustion, more consistent interaction with patients, and more stable team structure within shifts compared with the previous 24-hour call system. These workplace changes were felt to improve teamwork and patient ownership within shifts, quality of work performed, and empathy. Across shifts, however, more frequent sign-overs, stricter application of shift time boundaries, and loose integration between daytime and NF teams were perceived as leading to emergence of shift-worker mentality around sign-over. Perceptions of optimal patient ownership changed from the traditional single-physician-24/7 model to team-based shared ownership. Conclusions Duty hours restrictions, as exemplified by an NF system, have both positive and negative impacts on professionalism. Interventions and training toward effective team-based care are needed to curb emergence of shift-worker mentality

Identification of Sensitive Parameters for Deformation of Asphalt Concrete Face Rockfill Dam of Pumped Storage Power Station

Pumped storage power station (PSPS) is an important clean energy project that plays an important role in ensuring the economical, safe, and stable operation of power systems and alleviating the contradiction of peak load regulation. Deformation analysis of the built and under construction PSPS dam was an important process of dam design and operation, which was of great significance to ensure the safe operation of hydraulic structures in the reservoir site. Nevertheless, there were many parameters involved in the model for analyzing dam deformation, which brings a large workload to the inversion and application of model parameters. In this study, the asphalt concrete face rockfill dam (ACFRD) of a PSPS in Ningxia, China, was taken as an example, a dam deformation 3D finite element analysis model based on the Duncan–Chang E-B model was constructed, and the orthogonal test method was used. The model parameters of the main rockfill zone, secondary rockfill zone, and reservoir bottom backfill zone were taken as factors for the sensitivity analysis of horizontal displacement of dam H, vertical displacement u, and asphalt concrete face tensile strain ε. The results showed that initial bulk modulus base Kb, damage ratio Rf, and initial elastic modulus base K had a relatively higher sensitivity and had significant impacts on the calculation results, while internal friction angle φ, fraction angle reduction φ, bulk modulus index m, and elastic modulus index n had a relatively lower sensitivity, which had no significant impact on the calculation results. Therefore, when using the Duncan–Chang E-B model to analyze the deformations of a PSPS dam and asphalt concrete face, Kb, Rf, and K should be the focus. Parameters with a low sensitivity could be determined by engineering analogy so as to achieve the purpose of improving calculation efficiency under the premise of ensuring calculation accuracy. Meanwhile, these parameters should also be strictly controlled during construction. The results of this study could provide a reference for the design and safety assessment of ACFRD in PSPS

Identification of Sensitive Parameters for Deformation of Asphalt Concrete Face Rockfill Dam of Pumped Storage Power Station

Pumped storage power station (PSPS) is an important clean energy project that plays an important role in ensuring the economical, safe, and stable operation of power systems and alleviating the contradiction of peak load regulation. Deformation analysis of the built and under construction PSPS dam was an important process of dam design and operation, which was of great significance to ensure the safe operation of hydraulic structures in the reservoir site. Nevertheless, there were many parameters involved in the model for analyzing dam deformation, which brings a large workload to the inversion and application of model parameters. In this study, the asphalt concrete face rockfill dam (ACFRD) of a PSPS in Ningxia, China, was taken as an example, a dam deformation 3D finite element analysis model based on the Duncan–Chang E-B model was constructed, and the orthogonal test method was used. The model parameters of the main rockfill zone, secondary rockfill zone, and reservoir bottom backfill zone were taken as factors for the sensitivity analysis of horizontal displacement of dam H, vertical displacement u, and asphalt concrete face tensile strain ε. The results showed that initial bulk modulus base Kb, damage ratio Rf, and initial elastic modulus base K had a relatively higher sensitivity and had significant impacts on the calculation results, while internal friction angle φ, fraction angle reduction φ, bulk modulus index m, and elastic modulus index n had a relatively lower sensitivity, which had no significant impact on the calculation results. Therefore, when using the Duncan–Chang E-B model to analyze the deformations of a PSPS dam and asphalt concrete face, Kb, Rf, and K should be the focus. Parameters with a low sensitivity could be determined by engineering analogy so as to achieve the purpose of improving calculation efficiency under the premise of ensuring calculation accuracy. Meanwhile, these parameters should also be strictly controlled during construction. The results of this study could provide a reference for the design and safety assessment of ACFRD in PSPS

Dynamic analysis of a dielectric elastomer-based bistable system

Bistable structures, which are capable of oscillating between two stable states, have garnered significant attention and have been applied in many engineering domains due to their nonlinear behaviors. This paper presents for the first time a physical nonlinear bistable structure comprising a circular dielectric elastic membrane (DEM) coupled with four linear springs, enabling the DEM to achieve large deformations with the system oscillating between two stable equilibrium states. The nonlinear dynamics of the proposed bistable system under harmonic excitation is investigated through analytical, experimental, and numerical methods. First, the system structure is introduced and the corresponding dynamic model is established using Euler-Lagrange equations. Subsequently, the restoring forces of the DEM are experimentally measured, and the dynamic behavior of the bistable system is experimentally tested to validate the theoretical model. Furthermore, the dynamic responses of the system under different excitations are fully studied, and the influences of some key system parameters on the system response are analyzed. Research results demonstrate that the proposed structure exhibits bistable behavior under external excitation and the dynamic behavior of the system can be accurately predicted using the derived theoretical model. The proposed bistable structure contains rich dynamic behaviors including periodic motion, period-doubling bifurcations, and chaotic vibrations. The obtained parameters adjusting rules provide guidelines for the control of system's responses, which have potential applications in many fields such as vibration mitigation and energy harvesting

COVID-19 transmission in Mainland China is associated with temperature and humidity: A time-series analysis

COVID-19 has become a pandemic. The influence of meteorological factors on the transmission and spread of COVID-19 if of interest. This study sought to examine the associations of daily average temperature (AT) and relative humidity (ARH) with the daily count of COVID-19 cases in 30 Chinese provinces (in Hubei from December 1, 2019 to February 11, 2020 and in other provinces from January 20, 2020 to Februarys 11, 2020). A Generalized Additive Model (GAM) was fitted to quantify the province-specific associations between meteorological variables and the daily cases of COVID-19 during the study periods. In the model, the 14-day exponential moving averages (EMAs) of AT and ARH, and their interaction were included with time trend and health-seeking behavior adjusted. Their spatial distributions were visualized. AT and ARH showed significantly negative associations with COVID-19 with a significant interaction between them (0.04, 95% confidence interval: 0.004-0.07) in Hubei. Every 1°C increase in the AT led to a decrease in the daily confirmed cases by 36% to 57% when ARH was in the range from 67% to 85.5%. Every 1% increase in ARH led to a decrease in the daily confirmed cases by 11% to 22% when AT was in the range from 5.04°C to 8.2°C. However, these associations were not consistent throughout Mainland China

Sinonasal Inverted Papilloma–Associated and De Novo Squamous Cell Carcinoma: A Tale of Two Cities or Not

Background: Sinonasal squamous cell carcinoma (SNSCC) can arise as either inverted papilloma–associated SCC (IP-SCC) or as de novo SCC (DN-SCC). It is controversial as to whether survival differences between IP-SCC and DN-SCC exist. Methods: Between January 2000 and December 2016, 234 patients with SNSCC were analyzed retrospectively, including 68 with IP-SCC and 166 with DN-SCC. Propensity score matching (PSM) was performed to balance baseline characteristics. The Kaplan–Meier method and Cox proportional hazard model were used to determine risk factors on survival outcomes. Results: The median follow-up time was 98.4 months. Before PSM, lymph node metastasis was noted to be lower in patients with IP-SCC. After PSM, the 5-year DFS, DSS and OS between IP-SCC and DN-SCC were 43.0% vs. 44.5% (p = 0.701), 49.2% vs. 56.2% (p = 0.753), and 48.2% vs. 52.9% (p = 0.978). The annual hazards of local failure, respectively, peaked at 28.4% and 27.8% for IP-SCC and DN-SCC within 12 months after treatment. Afterward, the hazards gradually decreased and the hazard for IP-SCC was always higher before approaching null. Conclusions: This study provides novel evidence to support the clinical utility of improved distinction between IP-SCC and DN-SCC. Further studies are necessary to validate these findings before considering escalation of IP-SCC