การพัฒนารูปแบบการสอนงานของหัวหน้างานเพื่อการทำผลงานวิชาการสำหรับบุคลากรสายสนับสนุน

The objectives of this research were: 1) to develop and evaluate a supervisory coaching model, 2) to develop and evaluate the supervisory coaching manual, and 3) to follow up the use of the supervisory coaching manual for producing supporting staff’s academic work. The population and sample groups used in this research consisted of: 1) a sample group of 10 interviewees; 2) the population of 400 people were asked in the requirement gathering stage; 3) a sample of 46 people tested the proposed coaching manual; and 4) the population of 30 people tested the coaching process. The research found that: 1) the supervisory coaching model for producing academic work consisted of (1) a training package, (2) training modules, (3) components of the training modules; (4) training package measurements and evaluation, and (5) supervisors. The appropriateness of the manual was evaluated by five experts. It was found that the manual was appropriate at the highest level. 2) The resultant coaching manual consisted of 5 modules. They included: Module 1 – the writing and analytical techniques, Module 2 – the work synthesising techniques, Module 3 – the work manual writing techniques, Module 4 – the research techniques, and Module 5 – the teaching and training techniques. Each of the modules was comprised of the following components: description, objectives, content, activities, media as well as test and training evaluation. The coaching manual evaluation that was tested with supervisors showed that the training participants’ average mark was 10.39 before the training. After the training, their average mark increased to 17.80, which was statistically significant at the level of .05. The evaluation of the efficacy of the coaching model in each learning module showing the relationship between the coaching process and accomplishment 80/80 (E1/E2) demonstrated that, on average, the training participants accomplished higher than the specified criteria. The overall accomplishment was also higher than the criteria at 83.47/86.60. 3) The results of the follow-up revealed that subordinates were satisfied by the coaching of their supervisors for producing the academic work, with the average satisfactory score at the very high level. They have also used the knowledge to begin writing their own work manual, analytical work as well as their research

Surface modification of gold nanoparticles with neuron-targeted exosome for enhanced blood–brain barrier penetration

Gold nanoparticles (AuNPs) have been extensively used as nanomaterials for theranostic applications due to their multifunctional characteristics in therapeutics, imaging, and surface modification. In this study, the unique functionalities of exosome-derived membranes were combined with synthetic AuNPs for targeted delivery to brain cells. Here, we report the surface modification of AuNPs with brain-targeted exosomes derived from genetically engineered mammalian cells by using the mechanical method or extrusion to create these novel nanomaterials. The unique targeting properties of the AuNPs after fabrication with the brain-targeted exosomes was demonstrated by their binding to brain cells under laminar flow conditions as well as their enhanced transport across the blood brain barrier. In a further demonstration of their ability to target brain cells, in vivo bioluminescence imaging revealed that targeted-exosome coated AuNPs accumulated in the mouse brain after intravenous injection. The surface modification of synthetic AuNPs with the brain-targeted exosome demonstrated in this work represents a highly novel and effective strategy to provide efficient brain targeting and shows promise for the future in using modified AuNPs to penetrate the brain

Physicochemical evaluation of polymer-DNA complexes for DNA delivery

Available from British Library Document Supply Centre-DSC:DXN052493 / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo

Production and analysis of volatile flavor compounds in sweet fermented rice (Khao Mak)

Khao Mak is a sweet fermented rice-based dessert with a unique flavor profile commonly found throughout Thailand. The traditional starter culture (Look Pang) contains yeast, mold and herbs, which is used to ferment cooked glutinous rice. This research studied production of Khao Mak which resulted in volatile flavor compounds that were affected by rice varieties, including white glutinous rice (Kor Khor 6), Japanese rice (Hitomebore) and black glutinous rice (Kam Doi and Leum Phua). Total soluble solids (TSS) as degree Brix, pH, and alcohol concentrations were measured daily during the fermentation period. Volatile flavor compounds were separated and identified by gas chromatography mass spectrometry (GC-MS). At the end of the fermentation, samples had pH ranging from 3.91±0.16 to 4.30±0.09, total soluble solids of 32.65±1.65 to 44.02±1.72°Brix, and alcohol concentrations between 0.33±0.03 and 0.38±0.03% (v/v). The potent odors associated with Khao Mak were alcohol, wine-like, whiskey-like, solvent-like, sweet and fruity. The major volatile compounds, which have stimulant flavor in Khao Mak, included 1-propanal, 1-propanol-2-methyl, 1-butanal-3-methyl and acetic acid ethyl ester. Among all rice varieties tested, the white glutinous rice produced Khao Mak containing the highest TSS, alcohol concentrations followed by Hitomebore rice and black glutinous rice, respectively

Polypropylene/hemp woody core fiber composites: Morphology, mechanical, thermal properties, and water absorption behaviors

Natural fiber composites composed of polypropylene, maleic anhydride-graft-polypropylene, and hemp woody core fiber were produced by two-roll mill mixing. The hemp woody core fiber was treated by alkaline. The morphology of the polypropylene/hemp woody core fiber composites was studied by scanning electron microscopy technique. The mechanical and thermo-mechanical properties of the polypropylene/hemp woody core fiber composites were determined in tensile, flexural tests, and thermogravimetric analysis, respectively, and discussed. It was found that incorporation of hemp woody core fiber in polypropylene increased the tensile and flexural modulus. Note that the stiffness of short hemp woody core fiber/polypropylene composites was markedly improved. However, the flexural and impact strength of polypropylene/hemp woody core fiber composites was reduced compared to the neat polypropylene. Incorporation of hemp fiber increased the water uptake compared to the neat polypropylene. It was established that the Fick’s law was fairly applicable to the water absorption results. The stiffness and thermogravimetric analysis behavior of the polypropylene/hemp woody core fiber composites were markedly affected by the surface treatments

Surface and Adhesion Properties of a Softener Containing Fragrances Microencapsulated with Poly (Methyl Methacrylate) on Cotton, Polyester, and a Mixture of Cotton and Polyester Fabrics

The distribution and adhesion of microcapsules on fabric surfaces are crucial factors for the production of long-lasting fragrance textiles. The objective of this research was to study the adhesion property of a softener containing microencapsulated fragrances on fabrics. Pink fruity fragrance (PF), and white floral fragrance (WF) were encapsulated with poly (methyl methacrylate) or PMMA, using the micro-suspension photopolymerization method, to form PF-PMMA, and WF-PMMA microcapsules, respectively. The particle sizes and zeta potential of the capsules were determined. The PF-PMMA and WF-PMMA were added to the fabric softener before being applied to three types of fabrics, cotton, TK (polyester), and TC (a mixture of cotton and TK). Surface morphologies of the fabrics treated with the softener were studied by scanning electron microscope (SEM). Interactions between the microcapsules and the fabrics were studied using a contract angle measurement device, Fourier Transform Infrared (FTIR) spectrometer, and Raman microscope. The average size of PF-PMMA was 484.8 ± 4.0 nm, smaller than that of WF-PMMA (664.6 ± 2.9 nm). Cotton was found to be hydrophilic with a rough surface due to cellulose fibers, while TK surface was smooth and hydrophobic. The different fiber structures and surface properties of the fabrics gave rise to different adhesion behavior, evidenced by the contract angle and Raman microscopic data. After 60 days of storage, the microencapsulated fragrances were found to remain on the cotton surfaces, but that on the TC and the TK surfaces disappeared. The results illustrated the interaction between the fabric surface and the microcapsules encapsulated with fragrances, which affected their adhesion. The knowledge obtained can be applied to the development of household products with long-lasting fragrances

Virtual screening of ABCC1 transporter nucleotide-binding domains as a therapeutic target in multi drug resistant cancer

ABCC1 is a member of the ATP-binding Cassette super family of transporters, actively effluxes xenobiotics from cells. Clinically, ABCC1 expression is linked to cancer multidrug resistance. Substrate efflux is energised by ATP binding and hydrolysis at the nucleotide-binding domains (NBDs) and inhibition of these events may help combat drug resistance. The aim of this study is to identify potential inhibitors of ABCC1 through virtual screening of National Cancer Institute (NCI) compounds. A threedimensional model of ABCC1 NBD2 was generated using MODELLER whilst the X-ray crystal structure of ABCC1 NBD1 was retrieved from the Protein Data Bank. A pharmacophore hypothesis was generated based on flavonoids known to bind at the NBDs using PHASE, and used to screen the NCI database. GLIDE was employed in molecular docking studies for all hit compounds identified by pharmacophore screening. The best potential inhibitors were identified as compounds possessing predicted binding affinities greater than ATP. Approximately 5% (13/265) of the hit compounds possessed lower docking scores than ATP in ABCC1 NBD1 (NSC93033, NSC662377, NSC319661, NSC333748, NSC683893, NSC226639, NSC94231, NSC55979, NSC169121, NSC166574, NSC73380, NSC127738, NSC115534), whereas approximately 7% (7/104) of docked NCI compounds were predicted to possess lower docking scores than ATP in ABCC1 NBD2 (NSC91789, NSC529483, NSC211168, NSC318214, NSC116519, NSC372332, NSC526974). Analyses of docking orientations revealed P-loop residues of each NBD and the aromatic amino acids Trp653 (NBD1) and Tyr1302 (NBD2) were key in interacting with high-affinity compounds. On the basis of docked orientation and docking score the compounds identified may be potential inhibitors of ABCC1 and require further pharmacological analysis. ABBREVIATIONS: ABC - ATP-binding cassette, DHS - dehydrosilybin, MDR - multidrug resistance, NBD - nucleotide-binding domain, PDB - protein data bank