The instantly blocking-based fluorescent immunochromatographic assay for the detection of SARS-CoV-2 neutralizing antibody

IntroductionAt present, there is an urgent need for the rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies (NAbs) to evaluate the ability of the human body to resist coronavirus disease 2019 (COVID-19) after infection or vaccination. The current gold standard for neutralizing antibody detection is the conventional virus neutralization test (cVNT), which requires live pathogens and biosafety level-3 (BSL-3) laboratories, making it difficult for this method to meet the requirements of large-scale routine detection. Therefore, this study established a time-resolved fluorescence-blocking lateral flow immunochromatographic assay (TRF-BLFIA) that enables accurate, rapid quantification of NAbs in subjects.MethodsThis assay utilizes the characteristic that SARS-CoV-2 neutralizing antibody can specifically block the binding of the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein and angiotensin-converting enzyme 2 (ACE2) to rapidly detect the content of neutralizing antibody in COVID-19-infected patients and vaccine recipients.ResultsWhen 356 samples of vaccine recipients were measured, the coincidence rate between this method and cVNT was 88.76%, which was higher than the coincidence rate of 76.97% between cVNT and a conventional chemiluminescence immunoassay detecting overall binding anti-Spike-IgG. More importantly, this assay does not need to be carried out in BSL-2 or 3 laboratories.DiscussionTherefore, this product can detect NAbs in COVID-19 patients and provide a reference for the prognosis and outcome of patients. Simultaneously, it can also be applied to large-scale detection to better meet the needs of neutralizing antibody detection after vaccination, making it an effective tool to evaluate the immunoprotective effect of COVID-19 vaccines

The Function of MoGlk1 in Integration of Glucose and Ammonium Utilization in Magnaporthe oryzae

Hexokinases are conserved proteins functioning in glucose sensing and signaling. The rice blast fungus Magnaporthe oryzae contains several hexokinases, including MoHxk1 (hexokinase) and MoGlk1 (glucokinase) encoded respectively by MoHXK1 and MoGLK1 genes. The heterologous expression of MoGlk1 and MoHxk1 in Saccharomyces cerevisiae confirmed their conserved functions. Disruption of MoHXK1 resulted in growth reduction in medium containing fructose as the sole carbon source, whereas disruption of MoGLK1 did not cause the similar defect. However, the ΔMoglk1 mutant displayed decreased proton extrusion and a lower biomass in the presence of ammonium, suggesting a decline in the utilization of ammonium. Additionally, the MoGLK1 allele lacking catalytic activity restored growth to the ΔMoglk1 mutant. Moreover, the expression of MoPMA1 encoding a plasma membrane H+-ATPase decreased in the ΔMoglk1 mutant that can be suppressed by glucose and G-6-P. Thus, MoGlk1, but not MoHxk1, regulates ammonium utilization through a mechanism that is independent from its catalytic activity

Parallel groundwater flow simulation method based on a discrete fracture network model

Objective Groundwater flow in fractured rocks has strong heterogeneity and anisotropy. The discrete fracture network (DFN) method has been internationally considered as one of the most reasonable and effective methods to describe the fracture water transport. Methods In this work, we focused on granite rock from an underground research laboratory site for the geological disposal of high-level radioactive waste. In addition, a high-performance numerical computing system and parallel codes were employed to develop a groundwater flow simulation method in fractured rocks based on the DFN model. Results The results indicated that the proposed method could conduct the groundwater flow simulation of DFN with thousands of mesh elements. This improved the computational efficiency and ability of the parallel codes to deal with complex models. We established the DFN model structure optimization and parameter setting methods of boundary conditions in a complex condition. This could ensure that the hydraulic heads were continuous at different scale models. Furthermore, in the model area, the hydraulic head is distributed as a network structure along fractures. For the connected fractures, the water level was continuous and changed from high to low. However, the water level in the nonconnected fractures was discontinuous. Groundwater flows along the fracture from the high water level area to the low water level area. The connectivity and permeability of the fracture network have an obvious influence on the groundwater flow characteristics. Conclusion Therefore, we could conclude that the parallel groundwater flow simulation method based on the DFN model could more reasonably reflect the groundwater flow in a fractured rock mass. It was of great significance to further improve the simulated prediction ability and deepen the understanding of groundwater flow characteristics in a fractured medium

Evaluation of pars plana sclera fixation of posterior chamber intraocular lens

Purpose: The purpose of this study was to evaluate the clinical efficacy and safety of modified posterior chamber intraocular lens (PCIOL) implantation with transscleral fixation. Design and Setting: This is a study, which is conducted at Department of Ophthalmology, Jinan Eye Hospital, Jinan Second People′s Hospital. Materials and Methods: A total of 82 patients who were scheduled for sutured PCIOL were divided randomly into modified and conventional groups. The former underwent PCIOL through pars plana fixation with knot buried and without scleral flap and the latter underwent transscleral fixation of PCIOL in the ciliary sulcus. The main outcome measures included operative time, postoperative visual acuity, and postoperative complications. Results: The mean operative time of the modified group was 39.95 ± 5.87 min, which was significantly less than that of the conventional group (45.77 ± 5.21 min; P < 0.05). No difference was found in postoperative visual acuity between the two groups. There were no significant postoperative complications, including knot exposure, endophthalmitis, and retinal detachment in either group. The optical clamping of PCIOL was prone to occur in the conventional group. Conclusion: Modified sutured PCIOL implantation is a safe, effective, and feasible technique for the correction of aphakia in eyes without adequate posterior capsular support

Axial Compression Behavior of Symmetrical Full-Scale Concrete Filled Double Skin Steel Tube Stub Columns

To investigate the bearing behavior of symmetrical full-scale different strength concrete filled double skin steel tube (CFDST) stub columns, 19 full-scale specimens were designed, considering the slenderness ratio (&lambda;); the compression strength of core concrete and sandwich concrete (fcki, fcko), the thickness of the inner and outer steel tubes (ti, to); the diameter of inner and outer steel tubes (Di, Do); and the tensile strength of the inner and outer steel tubes (fyki, fyko) as the main parameters. Nonlinear constitutive models for concrete considering constraint effect were adopted, and a finite element (FE) model was established using ABAQUS software. By comparing the results between simulations and experiments, the rationality of the modeling method was verified. Based on the FE model, the parameter analysis for CFDST columns were conducted, and the force mechanism, stress distribution, and deformation process were analyzed. The results showed that the axial compression bearing capacity (Nsu) increased significantly with an increase in fcki, to, Do, and fcko, while Nsu decreased gradually with an increase in &lambda;. Finally, according to the calculated results of the specimens, the calculation formula for Nsu of full-scale composite columns was statistically regressed using 1stOpt software and showed a good agreement with the FE

Axial Compression Behavior of Symmetrical Full-Scale Concrete Filled Double Skin Steel Tube Stub Columns

To investigate the bearing behavior of symmetrical full-scale different strength concrete filled double skin steel tube (CFDST) stub columns, 19 full-scale specimens were designed, considering the slenderness ratio (λ); the compression strength of core concrete and sandwich concrete (fcki, fcko), the thickness of the inner and outer steel tubes (ti, to); the diameter of inner and outer steel tubes (Di, Do); and the tensile strength of the inner and outer steel tubes (fyki, fyko) as the main parameters. Nonlinear constitutive models for concrete considering constraint effect were adopted, and a finite element (FE) model was established using ABAQUS software. By comparing the results between simulations and experiments, the rationality of the modeling method was verified. Based on the FE model, the parameter analysis for CFDST columns were conducted, and the force mechanism, stress distribution, and deformation process were analyzed. The results showed that the axial compression bearing capacity (Nsu) increased significantly with an increase in fcki, to, Do, and fcko, while Nsu decreased gradually with an increase in λ. Finally, according to the calculated results of the specimens, the calculation formula for Nsu of full-scale composite columns was statistically regressed using 1stOpt software and showed a good agreement with the FE

Lateral-Torsional Buckling Analysis for Doubly Symmetric Tubular Flange Composite Beams with Lateral Bracing under Concentrated Load

Tubular flange composite beams are increasingly applied in modern bridge structures. In order to investigate the overall stability behavior of doubly symmetric tubular flange composite beams with lateral bracing under concentrated load, the analysis of elastic lateral-torsional buckling is conducted by the energy variation method. The analytical solution of critical moment of doubly symmetric tubular flange composite beams with lateral bracing is obtained. Meanwhile, the simplified calculation formula of critical moment is fitted by 1stOpt software based on 26,000 groups of data, and the accuracy is verified by the finite element method. It is found that, the critical moment rises obviously with increasing lateral bracing stiffness, and adding lateral bracing to doubly symmetric tubular flange composite beams is beneficial to improve the overall stability in engineering practice. Finally, the influence of several parameters including concrete strength, span, steel ratio of flange and height-thickness ratio of web are studied. The results show that the concrete strength and the web height-thickness ratio have a weak influence on critical moment of elastic lateral-torsional buckling, while the influence of span-depth ratio and flange steel ratio is very significant

The Efficacy of Hyperbaric Oxygen Therapy on Middle Cerebral Artery Occlusion in Animal Studies: A Meta-Analysis

<p>Point estimates and 95% CIs of effect size by: (A) HBO combined with drug therapy, (B) Repetitive or single HBO therapy. The 95% CI for the global estimate is shown as a grey band.</p