Media 2: Feature space optical coherence tomography based micro-angiography

Originally published in Biomedical Optics Express on 01 May 2015 (boe-6-5-1919

Media 1: Feature space optical coherence tomography based micro-angiography

Originally published in Biomedical Optics Express on 01 May 2015 (boe-6-5-1919

MIP view of UHS-OMAG images.

<p>(a) Before thinned-skull (baseline). (b) After thinned-skull cranial window procedure. (c) After open-skull cranial window procedure. (d–f) The blood perfusion images of the cross-sections located at dotted yellow lines. (g–i) The structural images of the same cross-sections. Scale bar is 0.3 mm.</p

Vessel density comparison and total blood flow quantification.

<p>(a) Comparison of detectable vessel density (n = 5). Baseline_1 refers to basal condition before the thinned skull cranial window and baseline_2 refers to basal condition before the open skull cranial window. (b) Comparison of total blood flow among penetrating arterioles in thinned skull and open skull with respect to intact skull case. (n = 6). There is no significant differences between thinned-skull case and open-skull case. Data represent mean±s.e.m. ***P<0.01, **P<0.05, and *P<0.3 significantly different data sets (paired t-test).</p

OCT Study of Mechanical Properties Associated with Trabecular Meshwork and Collector Channel Motion in Human Eyes - Fig 8

<p>OCT images (a-c) represent a section through the limbus while maintaining cannula pressures of 10, 30, and 50 mm Hg respectively; the images represent the static configuration after having switched to the respective reservoir pressures from a baseline of 0 mm Hg pressure. The ciliary body (CB), trabecular meshwork (TM), Schlemm’s canal (SC), collector channel (CC) and intrascleral collector channels (ISCC) undergo progressive changes in shape in response to pressure increases. Red asterisks indicate the base of a hinged collagen flap. Height changes with time of the lumen of SC, CC and ISCC are depicted in (d) (e) and (f); time was determined from the initiation of height change following switching from a baseline reservoir height of 0 to a height of 10, 30 or 50 mm Hg respectively. The bar chart (g) depicts maximum velocities of SC, CC and ISCC lumen height change following pressure changes from the 0 baseline to the10, 30, or 50 mm Hg reservoir height.</p

Schematic diagram of the experimental setup, including an SD-OCT system and a perfusion unit.

<p>The perfusion unit can be used to induce pressure transients. A single reservoir can control static pressures in the cannula inserted into Schlemm’s canal (SC), while switching between two reservoirs provides a means of examining dynamic changes. A radial limbal segment is placed with the trabecular meshwork facing upward toward the OCT imaging beam and a cannula is inserted into SC.</p

UHS-OMAG MIP view of microcirculation network up to ∼1 mm depth.

<p>(a–b) With intact skull (baseline). (c) With thinned-skull cranial window. (d) With open-skull cranial window. Scale bar represents 0.4 mm.</p

OCT cross-sectional images sequentially acquired at static progressively increasing perfusion pressures of 5, 10, 20 and 30 mmHg (top row) and then sequential acquisition in the reverse direction (bottom row).

<p>Ciliary Body (CB), TM Trabecular Meshwork (TM), Schlemm’s canal (SC), Collector Channel (CC).</p

(A), (B), and (C) are boxplots of the data for Schlemm’s canal (SC) area, height and height change respectively.

<p>Data is from 14 quadrants of 4 eyes in A & B and from 13 quadrants in C. SC height changes in C are reflected in the data of Fig 7, which displays normalized differences of individual quadrants. The boxplot central lines represent the median, and the box borders the interquartile distance. Means are represented by the small squares. Maximum value are shown as Δ, while minimum as inverted Δ. *p<0.05. The whiskers represent the 99% and 1% range of Tukey.</p