Healthy dietary patterns are associated with exposure to environmental chemicals in a pregnancy cohort

Healthy dietary patterns, such as the alternate Mediterranean diet and alternate Healthy Eating Index, benefit cardiometabolic health. However, several food components of these dietary patterns are primary sources of environmental chemicals. Here, using data from a racially and ethnically diverse US cohort, we show that healthy dietary pattern scores were positively associated with plasma chemical exposure in pregnancy, particularly for the alternate Mediterranean diet and alternate Healthy Eating Index with polychlorinated biphenyls and per- and poly-fluoroalkyl substances. The associations appeared stronger among Asian and Pacific Islanders. These findings suggest that optimizing the benefits of a healthy diet requires concerted regulatory efforts aimed at lowering environmental chemical exposure

Mutations in TUBB8 and Human Oocyte Meiotic Arrest

BACKGROUND Human reproduction depends on the fusion of a mature oocyte with a sperm cell to form a fertilized egg. The genetic events that lead to the arrest of human oocyte maturation are unknown. METHODS We sequenced the exomes of five members of a four-generation family, three of whom had infertility due to oocyte meiosis I arrest. We performed Sanger sequencing of a candidate gene, TUBB8, in DNA samples from these members, additional family members, and members of 23 other affected families. The expression of TUBB8 and all other β-tubulin isotypes was assessed in human oocytes, early embryos, sperm cells, and several somatic tissues by means of a quantitative reverse- transcriptase–polymerase-chain-reaction assay. We evaluated the effect of the TUBB8 mutations on the assembly of the heterodimer consisting of one α-tubulin polypeptide and one β-tubulin polypeptide (α/β-tubulin heterodimer) in vitro, on microtubule architecture in HeLa cells, on microtubule dynamics in yeast cells, and on spindle assembly in mouse and human oocytes. RESULTS We identified seven mutations in the primate-specific gene TUBB8 that were responsible for oocyte meiosis I arrest in 7 of the 24 families. TUBB8 expression is unique to oocytes and the early embryo, in which this gene accounts for almost all the expressed β-tubulin. The mutations affect chaperone-dependent folding and assembly of the α/β-tubulin heterodimer, disrupt microtubule behavior on expression in cultured cells, alter microtubule dynamics in vivo, and cause catastrophic spindle-assembly defects and maturation arrest on expression in mouse and human oocytes. CONCLUSIONS TUBB8 mutations have dominant-negative effects that disrupt microtubule behavior and oocyte meiotic spindle assembly and maturation, causing female infertility. (Funded by the National Basic Research Program of China and others.

Numerical Study on a Liquid Cooling Plate with a Double-Layer Minichannel for a Lithium Battery Module

The liquid cooling system of lithium battery modules (LBM) directly affects the safety, efficiency, and operational cost of lithium-ion batteries. To meet the requirements raised by a factory for the lithium battery module (LBM), a liquid cooling plate with a two-layer minichannel heat sink has been proposed to maintain temperature uniformity in the module and ensure it stays within the temperature limit. This innovative design features a single inlet and a single outlet. To evaluate the performance of the liquid cooling system, we considered various discharge rates while taking into account the structure, flow rate, and temperature of the coolant. Our findings indicate that at a mass outflow rate of 20 g/s, a better cooling effect and lower power consumption can be achieved. An inlet temperature of 20 °C, close to the initial temperature of the battery string, may be the most appropriate because a higher temperature of the coolant will cause a higher temperature of LBM, so far as to exceed the safe threshold value. In the case of larger rate discharge, the design of a double-layer MCHS at the bottom and an auxiliary one at the side can effectively reduce the maximum temperature LBM (within 28 °C) and maintain the temperature difference in the single cell at approximately 4 °C. In the case of non-constant discharges, the temperature difference between cells increases with the maximum temperature. When the discharge rate is reduced, the large temperature difference helps the temperature to drop rapidly. This can provide guidance for the design of cooling systems for the LBM

Determining the Image Base of Smart Device Firmware for Security Analysis

The authorization mechanism of smart devices is mainly implemented by firmware, yet many smart devices have security issues about their firmware. Limited research has focused on securing the firmware of smart devices, although increasingly more smart devices are used to deal with the very sensitive applications, activities, and data of users. Thus, research on smart device firmware security is of growing importance. Disassembly is a common method for evaluating the security of authorization mechanisms. When disassembling firmware, the processor type of the running environment and the image base of the firmware should first be determined. In general, the processor type can be obtained by tearing down the device or consulting the product manual. However, it is not easy to determine the image base of firmware. Since the processors of many smart devices are ARM architectures, in this paper, we focus on firmware under the ARM architecture and propose an automated method for determining the image base. By studying the storage law of the jump table in the firmware of ARM-based smart devices, we propose an algorithm, named determining the image base by searching jump tables (DBJT), to determine the image base. The experimental results indicate that the proposed method can successfully determine the image base of firmware, which stores the absolute address in the jump table

Energy-Efficient Multi-Task Allocation for Antenna Array Empowered Vehicular Fog Computing

Funding Information: This work was supported by the the National Key Research and Development Program of China under grant No. 2020YFB1806000. Publisher Copyright: © 2022 IEEE.With the emergence of compute-intensive and latency-sensitive vehicular applications, vehicular fog computing (VFC) has been proposed for catering to the thriving demands for computing and communication resources close to vehicles. In VFC scenarios where multiple tasks need to be offloaded simultaneously, the data, often coming from multiple sources, must be transmitted at a high data-rate in parallel. An antenna array system, a set of multiple connected antennas which work together as a single antenna, could achieve a significantly higher data-rate than a traditional single antenna. However, data-rate of the antenna array system may decrease due to the presence of interference. On the other hand, an antenna array system consumes more energy than a single antenna, which is antagonistic to vehicles powered by limited electricity. To address these challenges, we propose EAAV, a multi-task allocation strategy that enables multiple tasks to be offloaded concurrently in antenna array empowered VFC. EAAV aims at reducing the transmission power consumption while maintaining a high transmission data-rate, taking into account the mobility of vehicles and communication interference. We transform the multi-task allocation problem into a convex solvable one and evaluate the effectiveness of EAAV based on real-world vehicle trajectories. Compared with the existing task allocation strategy, EAAV improves the average transmission data-rate by up to 8.2% and reduces the average power consumption by up to 38.3%.Peer reviewe

Biotechnological advances for improving natural pigment production: a state-of-the-art review

In current years, natural pigments are facing a fast-growing global market due to the increase of people’s awareness of health and the discovery of novel pharmacological effects of various natural pigments, e.g., carotenoids, flavonoids, and curcuminoids. However, the traditional production approaches are source-dependent and generally subject to the low contents of target pigment compounds. In order to scale-up industrial production, many efforts have been devoted to increasing pigment production from natural producers, via development of both in vitro plant cell/tissue culture systems, as well as optimization of microbial cultivation approaches. Moreover, synthetic biology has opened the door for heterologous biosynthesis of pigments via design and re-construction of novel biological modules as well as biological systems in bio-platforms. In this review, the innovative methods and strategies for optimization and engineering of both native and heterologous producers of natural pigments are comprehensively summarized. Current progress in the production of several representative high-value natural pigments is also presented; and the remaining challenges and future perspectives are discussed.Published versionThis work was funded by the National Natural Science Foundation of China (22108097), Natural Science Foundation of Jiangsu Province (BK20200616), National Key Research and Development Program of China (Grant No. 2018YFA0901800), China Postdoctoral Science Foundation (2020M671339), and Zhejiang Provincial Natural Science Foundation of China (Grant No. LZ20B060002)

Protective effects of Liupao tea against high-fat diet/cold exposure-induced irritable bowel syndrome in rats

Liupao tea as a type of dark tea can relieve irritable bowel syndrome by regulating gut microbiota, but the mechanism has not been fully explained. An ultra-high performance liquid chromatography along with quadrupole time of flight tandem mass spectrometry was used to analyze the phytochemicals in Liupao tea. Then, we explored the effects of Liupao tea against IBS. From the results of chemical analysis, we identified catechins, polyphenols, amino acids, caffeine, polysaccharides and other components in Liupao tea. The open-field test, gastrointestinal function-related indexes, histochemical assays, measurements of cytokine and aquaporin 3 (AQP3), and determination of serum metabolites were utilized to monitor the physiological consequences of Liupao tea administration in rats with irritable bowel syndrome. The results showed that Liupao tea had a significant protective effect on irritable bowel syndrome. Liupao tea increased locomotive velocity while reducing interleukin-6, interleukin-1β, and tumor necrosis factor-α levels, as well as gastrointestinal injury. Moreover, Liupao tea increased the AQP3 levels of renal tissues but reduced the AQP3 levels of gastrointestinal tissues. Liupao tea reduced the Firmicutes/Bacteroides ratio and significantly reconstructed the microbial pattern. Liupao tea relieved irritable bowel syndrome by repairing gastrointestinal dysfunction, regulating the secretion of pro-inflammatory cytokines, modulating water metabolism, and restoring microbial homeostasis