Comparing Teachers\u27 and Students\u27 Perceptions of Bullying: Frequency, Location, and Intervention

Bullying is recognized as a significant problem in children and adolescents, and schools are a place where bullying often occurs. For this reason, schools have used programs, such as the Olweus Bullying Prevention Program (OBPP), to increase awareness of bullying and means of intervening. The purpose of this qualitative study is to gain insight into the perspectives of teachers and students on perceived aspects of the bullying problem within the school building and environment in a school where a new bullying prevention program was being implemented. The study examines differing perspectives between teachers and students regarding the frequency of occurrence, location, and teacher intervention in bullying situations. Data was gathered via survey questions and interviews from a sample of students, teachers trained in the new bullying prevention program, and teachers without such training. The outcomes of this study shed light upon various areas in which teacher perceptions of bullying and student perceptions of bullying differ. More specifically, there are vast differences in the ways that teachers and students perceive the frequency of bullying, the hot spots for bullying, and how often teachers intervene. Knowledge of such differences of opinion could be a valuable component of teacher training in a bullying prevention program

SARS-CoV-2 Brain Regional Detection, Histopathology, Gene Expression, and Immunomodulatory Changes in Decedents with COVID-19

Brains of 42 COVID-19 decedents and 107 non-COVID-19 controls were studied. RT-PCR screening of 16 regions from 20 COVID-19 autopsies found SARS-CoV-2 E gene viral sequences in 7 regions (2.5% of 320 samples), concentrated in 4/20 subjects (20%). Additional screening of olfactory bulb (OB), amygdala (AMY) and entorhinal area for E, N1, N2, RNA-dependent RNA polymerase, and S gene sequences detected one or more of these in OB in 8/21 subjects (38%). It is uncertain whether these RNA sequences represent viable virus. Significant histopathology was limited to 2/42 cases (4.8%), one with a large acute cerebral infarct and one with hemorrhagic encephalitis. Case-control RNAseq in OB and AMY found more than 5000 and 700 differentially expressed genes, respectively, unrelated to RT-PCR results; these involved immune response, neuronal constituents, and olfactory/taste receptor genes. Olfactory marker protein-1 reduction indicated COVID-19-related loss of OB olfactory mucosa afferents. Iba-1-immunoreactive microglia had reduced area fractions in cerebellar cortex and AMY, and cytokine arrays showed generalized downregulation in AMY and upregulation in blood serum in COVID-19 cases. Although OB is a major brain portal for SARS-CoV-2, COVID-19 brain changes are more likely due to blood-borne immune mediators and trans-synaptic gene expression changes arising from OB deafferentation.</br