90 research outputs found

    Factors affecting the heat coagulation of homogenized coffee cream

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    Cover title.Bibliography: p. 578

    Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum

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    The Systemic Inflammatory Response Syndrome (SIRS), a sepsis related inflammatory state, is a self-defense mechanism against specific and nonspecific stimuli. The six most extensively studied inflammatory biomarkers for the clinical diagnosis of SIRS are interleukin 4 (hIL-4), interleukin 6 (hIL-6), interleukin 10 (hIL-10), tumor necrosis factor alpha (hTNF-a), interferon gamma (hIFN-gamma) and procalcitonin (hPCT). These biomarkers are naturally present (but usually only at low concentration) in SIRS infected patients [1, 2] and thus the development of a highly sensitive detection method is of major clinical interest. However, the existing analytical techniques are lacking in required analytical sensitivity and parallel determination of these biomarkers. We developed a fast, easy and cost-efficient protein microarray biochip where the capture molecules are attached on hydrogel spots, enabling SIRS diagnosis by parallel detection of these six clinically relevant biomarkers with a sample volume of 25 mu l. With our hydrogel based protein microarray biochip we achieved a limit of detection for hIL-4 of 75.2 pg/ml, for hIL-6 of 45.1 pg/ml, for hIL-10 of 71.5 pg/ml, for hTNF-alpha of 56.7 pg/ml, for IFN-gamma of 46.4 pg/ml and for hPCT of 1.1 ng/ml in spiked human serum demonstrating sufficient sensitivity for clinical usage. Additionally, we demonstrated successful detection of two relevant SIRS biomarkers in clinical patient samples with a turnaround time of the complete analysis from sample-to-answer in less than 200 minutes

    Care of the cream separator

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    Operation of the cream-buying station

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    The construction of the dairy house

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    The cream-buying station : a guide for the operator

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    Cover title.This circular replaces circular 487

    Recoil polarization measurements of the proton electromagnetic form factor ratio to high momentum transfer

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    Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Physics, 2010.Cataloged from PDF version of thesis.Includes bibliographical references (p. 305-313).The electromagnetic form factors of the nucleon characterize the effect of its internal structure on its response to an electromagnetic probe as studied in elastic electronnucleon scattering. These form factors are functions of the squared four-momentum transfer Q2 between the electron and the proton. The two main classes of observables of this reaction are the scattering cross section and polarization asymmetries, both of which are sensitive to the form factors in different ways. When considering large momentum transfers, double-polarization observables offer superior sensitivity to the electric form factor. This thesis reports the results of a new measurement of the ratio of the electric and magnetic form factors of the proton at high momentum transfer using the recoil polarization technique. A polarized electron beam was scattered from a liquid hydrogen target, transferring polarization to the recoiling protons. These protons were detected in a magnetic spectrometer which was used to reconstruct their kinematics, including their scattering angles and momenta, and the position of the interaction vertex. A proton polarimeter measured the polarization of the recoiling protons by measuring the azimuthal asymmetry in the angular distribution of protons scattered in CH2 analyzers. The scattered electron was detected in a large-acceptance electromagnetic calorimeter in order to suppress inelastic backgrounds. The measured ratio of the transverse and longitudinal polarization components of the scattered proton is directly proportional to the ratio of form factors GE/Gpm. The measurements reported in this thesis took place at Q2 =5.2, 6.7, and 8.5 GeV 2, and represent the most accurate measurements of G' in this Q2 region to date.by Andrew James Ruehe Puckett.Ph.D

    Sweet-curd cottage cheese : directions for manufacturing with an enzyme

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    Operation of the cream receiving station

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