5 research outputs found
Detection of the beta-lactamase gene in commercial Taq polymerase.
<p>Four dilutions of Amplitaq DNA polymerase were tested with primers for the beta-lactamase gene in the presence of 10<sup>3</sup> pUC19 plasmids (labeled 1000 pUC19 genomes) or H<sub>2</sub>O.</p
Detection of <i>Pseudomonas fluorescens</i> at high and low Taq polymerase concentrations.
<p>Bacterial detection with 0.5 Units (A) or 0.05 Units (B) Qiagen Taq DNA polymerase with 16S350B assay on samples containing 10<sup>3</sup>, 10<sup>2</sup>, 10<sup>1</sup> and zero <i>Pseudomonas fluorescens</i> bacteria. A composite of A and B is shown in C.</p
Approximating the copy number of 16S rDNA in commercial Taq polymerases.
<p>Six DNA polymerases were used with primers for 16S rDNA on 7 serial 10-fold dilutions of E. coli genomic DNA (10 ng to 10 fg) with no added DNA in the 8<sup>th</sup> sample. The least squares fit equation for each dilution series was used to assign a value to the signal from the 8<sup>th</sup> sample, which contains Taq-associated DNA only. The efficiency of the reaction was determined from the slope of the linear fit plotting the base10 log of the DNA concentration vs. the threshold cycle. A slope of β3.322 indicates an average doubling rate of β2,β which is approximately 100% efficiency (2Μ3.322βΌ10). The rDNA values assigned are for βE. coli equivalents.β</p
Detection of bacterial DNA in six Taq polymerases.
<p>Four dilutions of six DNA polymerase were tested with primers for 16S rDNA in the presence of 100 pg (βΌ10Μ5 16S rDNA) E. coli genomic DNA (circled) or H<sub>2</sub>O.</p
Alignment of 16S primers on the genomes of 12 bacterial sequences.
<p>Four bacteria, <i>M.luteus, S.griseus, V.fischeri</i> and <i>M.catarrhalis</i> were detected at least at 100-fold greater levels with LP2 than with LP1, presumably due to the lack of basepairing of the 3β² base of LP1. The key beneath the figure provides the GenBank records for the sequences shown.</p