8 research outputs found
Overexpression, purification, characterization and preliminary crystallographic study of Âphosphoglycolate phosphatase from Shigella flexneri 2a strain 301
Recombinant phosphoglycolate phosphatase from S. flexneri was overexpressed, purified, characterized and crystallized using the hanging-drop vapour-diffusion method. SeMet-labelled protein was also prepared and was crystallized for phase determination using the MAD technique