8 research outputs found

    Overexpression, purification, characterization and preliminary crystallographic study of ­phosphoglycolate phosphatase from Shigella flexneri 2a strain 301

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    Recombinant phosphoglycolate phosphatase from S. flexneri was overexpressed, purified, characterized and crystallized using the hanging-drop vapour-diffusion method. SeMet-labelled protein was also prepared and was crystallized for phase determination using the MAD technique
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