Circulating parameters of non-esterified fatty acids (NEFAs), triglycerides (TG), cholesterol, insulin, leptin and basal glucose in WT and Nur77 KO mice after 16 wk of HFD.

<p>n = 7–8 per group. *p<0.05.</p

High fat diet changes body weight, adiposity, and energy expenditure in female Nur77 KO mice.

<p>(A) Body weight of male and female mice after 16 wk of free access to HFD. (B) Profile of body weight during 14 weeks on HFD. (C) Fat mass in grams. (D) Fat mass as percentage of body weight. (E) Fat-free mass in grams. (F) Fat-free mass as percentage of body weight. (G) Cumulative food intake over 48 hr. (H) 48 hr total energy expenditure determined by indirect calorimetry. Measurements were taken every 45 minutes. Grey squares indicate lights-off. (I) Energy expenditure corrected by kg of fat free mass. (J) Locomotor activity during a 48 hr period. (K) Average of respiratory quotient during 48h. (L) Respiratory quotient during light phase. (M) Respiratory quotient during dark phase. Mice were ∼5 months of age at time of measurement. *p<0.05, n = 7–8 per group.</p

Effect of Nur77 deficiency in the muscle of mice fed with HFD.

<p>(A) Total muscle triglyceride (TG) content after HFD exposure. (B) Representative western blot of protein levels of AKT, pAKT and PTEN in the muscle of male and female WT and Nur77 KO mice. Dividing lines indicate splicings in the figure. *p<0.05, **p<0.01, n = 7–8 per group.</p

Glucose tolerance and insulin sensitivity in Nur77 KO mice.

<p>(A) Intraperitoneal glucose tolerance test (ipGTT) in males after 16 wk of HFD. (B) Respective area under the curve (AUC). (C) ipGTT in females after 16 wk of HFD. (D) Respective AUC. (E) Insulin tolerance test (ITT) in males after 16 wk of HFD. (F) Respective AUC. (G) ITT in females after 16 wk of HFD. (H) Respective AUC. (I) Delta glycemia during an ITT in males after 16 wk of HFD. (J) Respective AUC. (K) Delta glycemia during an ITT in females after 16 wk of HFD. (L) Respective AUC. *p<0.05, **p<0.01, n = 7–8 per group.</p

Lipid metabolism in gonadal white adipose tissue in Nur77 KO mice fed with HFD.

<p>(A) mg TG content per gram of WAT. (B) mg TG content in the total amount of WAT. (C) mRNA expression of enzymes involved in lipid storage: SREBP1, ACCα, FAS, and LPL in white adipose tissue after 16 wk of HFD. (D) Protein levels of HSL, pHSL, and the ratio pHSL/HSL. Dividing lines indicate splicings in the figure. *p<0.05, n = 7–8 per group.</p

Effect of Nur77 deficiency in the liver of mice fed with HFD.

<p>(A) Total liver triglyceride (TG) content after HFD exposure. mRNA expression of SREBP1, CPT1-L and ACCα (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053836#pone-0053836-g002" target="_blank">Figure 2B</a>) in the liver of WT and Nur77 KO mice. Representative western blot of protein levels of (C) FAS, (D) pJNK1 and (E) JNK1 in the liver of WT and Nur77 KO mice. Dividing lines indicate splicings in the figure. *p<0.05, **p<0.01, n = 7–8 per group.</p

Glucose homeostasis and insulin sensitivity in mice fed a standard diet.

<p>Basal glucose levels (A), glucose tolerance test (B), respective area under the curve (C), insulin tolerance test (% of glucose levels represented against t0) (D), and respective area under the curve (E) in 8-week male wild type (WT) and endoglin heterozygous (HZ) mice fed a standard diet. n = 6–8. *p<0.05.</p

Levels of plasma metabolites and hormones in mice fed a high fat diet.

<p>Serum levels of insulin (A), glucose (B), TG (C), NEFAS (D), and cholesterol (E) in male wild type and endoglin heterozygous mice fed a high fat diet for 16 weeks. n = 6–8. **p<0.01.</p

Hepatic glucose production in mice fed a high fat diet.

<p>mRNA levels of PEPCK and G6Pase (A) and triglyceride (TG) content (B) in the liver of male wild type and endoglin heterozygous mice fed a high fat diet for 16 weeks. 18S was used as an internal control. n = 6–8. **p<0.01.</p

Glucose homeostasis and insulin sensitivity in mice fed a high fat diet.

<p>Basal glucose levels (A), glucose tolerance test (B), respective area under the curve (C), insulin tolerance test (% of glucose levels represented against t0) (D), and respective area under the curve (E) in male wild type (WT) and endoglin heterozygous (HZ) mice fed a high fat diet for 16 weeks. n = 6–8. *p<0.05.</p