Nonlinear Criterion for the Stability of Molecular Clouds

Dynamically significant magnetic fields are routinely observed in molecular clouds, with mass-to-flux ratio lambda = (2 pi sqrt{G}) (Sigma/B) ~ 1 (here Sigma is the total column density and B is the field strength). It is widely believed that ``subcritical'' clouds with lambda < 1 cannot collapse, based on virial arguments by Mestel and Spitzer and a linear stability analysis by Nakano and Nakamura. Here we confirm, using high resolution numerical models that begin with a strongly supersonic velocity dispersion, that this criterion is a fully nonlinear stability condition. All the high-resolution models with lambda <= 0.95 form ``Spitzer sheets'' but collapse no further. All models with lambda >= 1.02 collapse to the maximum numerically resolvable density. We also investigate other factors determining the collapse time for supercritical models. We show that there is a strong stochastic element in the collapse time: models that differ only in details of their initial conditions can have collapse times that vary by as much as a factor of 3. The collapse time cannot be determined from just the velocity dispersion; it depends also on its distribution. Finally, we discuss the astrophysical implications of our results.Comment: 11 pages, 5 figure

Adhesive-based selection by a tentacle-feeding polychaete for particle size, shape and bacterial coating in silt and sand

We tested particle selection by a surface deposit-feeding, tentaculate spionid polychaete, Pseudopolydora kempi japonica Imajima and Hartman. In experiments with peroxide-cleaned, sizegraded but otherwise natural silts and sands, individual worms showed peak preference for particles 80-99 μm in diameter, compared with previous (Self and Jumars, 1988) documentation of peak preference for particles of 7 μm in experiments conducted with plastic and glass beads. These results imply that microtektites will not in general be good tracers of mixing of mineral grains of comparable size. Animals exhibited statistically significant but not marked differences in size selectivity for subrounded versus subangular grain shapes; the size preference peak was broader in subangular grains, for which orientation of the grain can alter probabilities of both contact and retention. When one size class of grains was coated with the bacterium Halomonas halodurans (ATCC 29686), animals in general showed enhanced selection (relative to controls with no food value on any size class) of that size and smaller grains, even though these smaller grains lacked food value. Greatest selection, however, generally occurred for the coated size class. Results from inclusion of glass beads in some of the experiments and from separate experiments with tentacle analogs imply that this selective capability may be largely passive and mechanical. Natural grains, due to surface texture, have more surface area for adhesive contact than do smooth glass beads so that larger grains than beads are retained. Bacteria-coated grains, in turn, appear to be picked up preferentially due to adhesion with the bacterial coating; petroleum jelly-coated microscope slides also succeed in selective retention of the size class that is bacterially coated. Substantial selection by adhesive-utilizing deposit feeders apparently can be achieved without investment in complex, time- and energy-consuming sensory systems and behaviors. Experiments with natural grains showed notably more scatter than prior experiments with glass beads, but this difference is consistent with the mechanism. For nonspherical particles, both contact and retention depend on orientation as well as size

Extracellular antifreeze protein significantly enhances the cryopreservation of cell monolayers

The cryopreservation of cells underpins many areas of biotechnology, healthcare, and fundamental science by enabling the banking and distribution of cells. Cryoprotectants are essential to prevent cold-induced damage. Here, we demonstrate that extracellular localization of antifreeze proteins can significantly enhance post-thaw recovery of mammalian cell monolayers cryopreserved using dimethyl sulfoxide, whereas they show less benefit in suspension cryopreservation. A type III antifreeze protein (AFPIII) was used as the macromolecular ice recrystallization inhibitor and its intra/extracellular locations were controlled by using Pep-1, a cell-penetrating peptide. Flow cytometry and confocal microscopy confirmed successful delivery of AFPIII. The presence of extracellular AFPIII dramatically increased post-thaw recovery in a challenging 2-D cell monolayer system using just 0.8 mg·mL–1, from 25% to over 60%, whereas intracellularly delivered AFPIII showed less benefit. Interestingly, the antifreeze protein was less effective when used in suspension cryopreservation of the same cells, suggesting that the cryopreservation format is also crucial. These observations show that, in the discovery of macromolecular cryoprotectants, intracellular delivery of ice recrystallization inhibitors may not be a significant requirement under “slow freezing” conditions, which will help guide the design of new biomaterials, in particular, for cell storage

Semimetalic antiferromagnetism in the half-Heusler compound CuMnSb

The half-Heusler compound CuMnSb, the first antiferromagnet (AFM) in the Mn-based class of Heuslers and half-Heuslers that contains several conventional and half metallic ferromagnets, shows a peculiar stability of its magnetic order in high magnetic fields. Density functional based studies reveal an unusual nature of its unstable (and therefore unseen) paramagnetic state, which for one electron less (CuMnSn, for example) would be a zero gap semiconductor (accidentally so) between two sets of very narrow, topologically separate bands of Mn 3d character. The extremely flat Mn 3d bands result from the environment: Mn has four tetrahedrally coordinated Cu atoms whose 3d states lie well below the Fermi level, and the other four tetrahedrally coordinated sites are empty, leaving chemically isolated Mn 3d states. The AFM phase can be pictured heuristically as a self-doped Cu$^{1+}$Mn$^{2+}$Sb$^{3-}$ compensated semimetal with heavy mass electrons and light mass holes, with magnetic coupling proceeding through Kondo and/or antiKondo coupling separately through the two carrier types. The ratio of the linear specific heat coefficient and the calculated Fermi level density of states indicates a large mass enhancement $m^*/m \sim 5$, or larger if a correlated band structure is taken as the reference

Phase vortices from a Young's three-pinhole interferometer

An analysis is presented of the phase vortices generated in the far field, by an arbitrary arrangement of three monochromatic point sources of complex spherical waves. In contrast with the case of three interfering plane waves, in which an infinitely-extended vortex lattice is generated, the spherical sources generate a finite number of phase vortices. Analytical expressions for the vortex core locations are developed and shown to have a convenient representation in a discrete parameter space. Our analysis may be mapped onto the case of a coherently-illuminated Young's interferometer, in which the screen is punctured by three rather than two pinholes.Comment: 10 pages, 8 figures, REVTeX4, Submitted to Phys. Rev.

Designing a Replication Study in Kinesiology: Lessons from the Field

The submitted presentation material summarizes a project presented at the 2021 Cal Poly Virtual BEACoN Symposium. The title of the project which the presentation is based is, “Towards Equitable Communication: Explorations to Guide Knowledge Translation in Kinesiology.” The uploaded file document presents the presentation abstract, student testimony, as well as suggested citations for individual aspects of the presentation material. Please follow the social media profiles of the faculty mentor to the project, Dr. Thomas, for timely project updates. You may find related work from this lab group published to Cal Poly Digital Commons under the Kinesiology and Public Health section (see URL): https://digitalcommons.calpoly.edu/kinesp/ . Finally, a copy of the video presentation itself has been attached. Patrons are encouraged to use the file itself in their work. The video has English subtitles

Engineering cell surfaces by covalent grafting of synthetic polymers to metabolically-labeled glycans

Re-engineering mammalian cell surfaces enables modulation of their phenotype, function, and interactions with external markers and may find application in cell-based therapies. Here we use metabolic glycan labeling to install azido groups onto the cell surface, which can act as anchor points to enable rapid, simple, and robust “click” functionalization by the addition of a polymer bearing orthogonally reactive functionality. Using this strategy, new cell surface functionality was introduced by using telechelic polymers with fluorescence or biotin termini, demonstrating that recruitment of biomacromolecules is possible. This approach may enable the attachment of payloads and modulation of cell function and fate, as well as providing a tool to interface synthetic polymers with biological systems

Experimental study of out of equilibrium fluctuations in a colloidal suspension of Laponite using optical traps

This work is devoted to the study of displacement fluctuations of micron-sized particles in an aging colloidal glass. We address the issue of the validity of the fluctuation dissipation theorem (FDT) and the time evolution of viscoelastic properties during aging of aqueous suspensions of a clay (Laponite RG) in a colloidal glass phase. Given the conflicting results reported in the literature for different experimental techniques, our goal is to check and reconcile them using \emph{simultaneously} passive and active microrheology techniques. For this purpose we measure the thermal fluctuations of micro-sized brownian particles immersed in the colloidal glass and trapped by optical tweezers. We find that both microrheology techniques lead to compatible results even at low frequencies and no violation of FDT is observed. Several interesting features concerning the statistical properties and the long time correlations of the particles are observed during the transition