Political Polupragmones: Busybody Athenians, Meddlesome Citizenship, and Epistemic Democracy in Classical Athens

The figure of the πολυπράγμων, the overactive, over-engaged, or meddlesome democratic citizen, is a literary trope that emerges in Classical Athenian literature in the 5th and 4th centuries B.C. This project seeks to use the πολυπράγμων as an entry point into understanding Athenian attitudes toward citizenship and socially acceptable political behaviors in Athens’ democratic era. I explore the history and usage of the term πολυπράγμων, and the associated characteristic of πολυπραγμοσύνη (meddlesomeness), and its synonyms and antecedents. I demonstrate that to be labeled πολυπράγμων is a term of social restraint—one is named a πολυπράγμων if they do not “mind their own business.” In 5th century Athens such an admonition is primarily political. It refers to and demonstrates the existence of a contested definition of what is and what is not acceptable political behavior on behalf of the non-elite citizens of Athens. Through a reading of Plato’s dialogues and an analysis of other Athenian literary productions describing street-level social and political interactions in the fourth century, I endeavor to demonstrate in the second half of this thesis that the behaviors of social inquisitiveness, over-activity, and the negative characteristics attributed to the πολυπράγμων by contemporary writers such as Plato, could actually have served to increase the common knowledge and cohesiveness of the Athenian city-state. To do this, I consider the πολυπράγμων through the lens of modern scholarship and social science that considers Athens as an “epistemic democracy” concerned with aggregating and employing politically useful information

Persistent DNA Damage after High Dose In Vivo Gamma Exposure of Minipig Skin

Exposure to high doses of ionizing radiation (IR) can lead to localized radiation injury of the skin and exposed cells suffer dsDNA breaks that may elicit cell death or stochastic changes. Little is known about the DNA damage response after high-dose exposure of the skin. Here, we investigate the cellular and DNA damage response in acutely irradiated minipig skin.IR-induced DNA damage, repair and cellular survival were studied in 15 cm(2) of minipig skin exposed in vivo to ~50 Co-60 γ rays. Skin biopsies of control and 4 h up to 96 days post exposure were investigated for radiation-induced foci (RIF) formation using γ-H2AX, 53BP1, and active ATM-p immunofluorescence. High-dose IR induced massive γ-H2AX phosphorylation and high 53BP1 RIF numbers 4 h, 20 h after IR. As time progressed RIF numbers dropped to a low of <1% of keratinocytes at 28-70 days. The latter contained large RIFs that included ATM-p, indicating the accumulation of complex DNA damage. At 96 days most of the cells with RIFs had disappeared. The frequency of active-caspase-3-positive apoptotic cells was 17-fold increased 3 days after IR and remained >3-fold elevated at all subsequent time points. Replicating basal cells (Ki67+) were reduced 3 days post IR followed by increased proliferation and recovery of epidermal cellularity after 28 days.Acute high dose irradiation of minipig epidermis impaired stem cell replication and induced elevated apoptosis from 3 days onward. DNA repair cleared the high numbers of DBSs in skin cells, while RIFs that persisted in <1% cells marked complex and potentially lethal DNA damage up to several weeks after exposure. An elevated frequency of keratinocytes with persistent RIFs may thus serve as indicator of previous acute radiation exposure, which may be useful in the follow up of nuclear or radiological accident scenarios