Schematic drawing of the profile area (grey region) based on membrane profile.

<p>Schematic drawing of the profile area (grey region) based on membrane profile.</p

Average profiles for the phantom with thickness 15 nm, with variable <i>μ</i>_<i>m</i>.

<p>G: Upper profile area with variable <i>μ</i>.</p

(a) Reconstruction from simulated X-ray microscope data and overlaid with known contours from the ideal geometry; (b) <i>left</i>: XY slice overlaid with the segmentation, using the found compatible ridges only (<i>green</i>) or using all ridges (<i>red</i>); <i>middle</i> and <i>right</i>: YZ and XZ slices overlaid with segmentation results before (<i>white</i>) and after (<i>red</i>) the refinement step for the membrane of organelle 1; (c) reconstruction overlaid with the final segmentation.

<p>(a) Reconstruction from simulated X-ray microscope data and overlaid with known contours from the ideal geometry; (b) <i>left</i>: XY slice overlaid with the segmentation, using the found compatible ridges only (<i>green</i>) or using all ridges (<i>red</i>); <i>middle</i> and <i>right</i>: YZ and XZ slices overlaid with segmentation results before (<i>white</i>) and after (<i>red</i>) the refinement step for the membrane of organelle 1; (c) reconstruction overlaid with the final segmentation.</p

<i>left</i>: Grouped bar plot of average segmentation error and standard deviation, calculated as surface distance, from the results with compatible ridges only and with all ridges; <i>right</i>: ideal geometry of the phantom, Color-coded segmentation errors from the results with compatible ridges only and with all ridges.

<p><i>left</i>: Grouped bar plot of average segmentation error and standard deviation, calculated as surface distance, from the results with compatible ridges only and with all ridges; <i>right</i>: ideal geometry of the phantom, Color-coded segmentation errors from the results with compatible ridges only and with all ridges.</p

Schematic drawing of the cryo-TXM optical settings.

<p>Schematic drawing of the cryo-TXM optical settings.</p